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Controlled release type yeast cell microcapsule product and preparation method thereof

A technology of yeast cells and microcapsules, which is applied in the direction of microcapsule preparation, microsphere preparation, botanical equipment and methods, etc., and can solve the problems of low affinity of water-soluble substances

Inactive Publication Date: 2015-07-29
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, its lipophilicity is strong, and its affinity for water-soluble substances is not high

Method used

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  • Controlled release type yeast cell microcapsule product and preparation method thereof
  • Controlled release type yeast cell microcapsule product and preparation method thereof
  • Controlled release type yeast cell microcapsule product and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Shake flask cultures of yeast cells were performed as previously described, and the yeast were harvested, washed, and freeze-dried.

[0071] After contacting 1g of yeast cells with 1-20mL 1% avermectin ethanol solution at 20-40°C for 24 hours at high frequency, centrifuge, wash off the unembedded avermectin on the surface, freeze-dry and grind thin. The embedding degree of abamectin in the microcapsules was determined to be 3.33% by high performance liquid chromatography.

Embodiment 2

[0075] As mentioned above, culture yeast cells in shake flasks, after centrifugal washing, add 0.5-10% sodium hydroxide by weight, perform shaking treatment at 40-60°C for 24-48 hours, and re-suspend after centrifugal washing Continue shaking treatment in 0.1-10% sodium hydroxide solution at a temperature of 40-60° C. for 24-48 hours, centrifuge, wash, and freeze-dry.

[0076] After 1 g of yeast cells and 1-20 mL of 1% abamectin ethanol solution are contacted at a high frequency at 20-40° C. for 24 hours, they are centrifuged, washed with water to remove unembedded abamectin on the surface, and then freeze-dried.

Embodiment 3

[0080] The steps of culturing, treating yeast and embedding are the same as in Example 1, except that 1-20 mL of 1% quizalofop-p-ethyl ethanol solution is added. The embedding degree of quizalofop-p-ethyl in the obtained microcapsules was 6.75%.

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PUM

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Abstract

The invention provides a controlled release type yeast cell microcapsule product and a preparation method thereof and relates to culture and treatment of yeast cells. A microcapsule wall material is obtained and subjected to hole sealing modification, and the microcapsule product is obtained. Yeast cells subjected to shake-flask culture under the proper condition are treated with chemical reagents such as lauryl sodium sulfate, dithiothreitol and the like, centrifuged, washed and then further treated with a sodium hydroxide solution. The yeast cells are centrifuged, washed and freeze-dried. After the yeast cell wall material and a to-be-embedded core substance solution are contacted at high frequency for encapsulation, hole sealing modification with cationic polymers is performed. Centrifugation is performed, the unencapsulated core substance and redundant cationic polymers on the surface are washed away, grinding is performed after freeze-drying, and the controlled release type yeast cell microcapsule which is uniform in size and not sticky is obtained.

Description

technical field [0001] The invention provides a method for preparing controlled-release yeast cell microcapsules, which relates to methods for culturing and treating yeast cells, preparing microcapsules and sealing holes, and further relates to microcapsule wall materials and microcapsules prepared by the method. Capsule product. Background technique [0002] Microcapsule technology wraps solid, liquid or gas (core substance) in another material (wall material), which can protect the core substance from the environment and control the release of the core substance. One of the key high-tech development. Encapsulation efficiency and burst release rate are the two most important indicators for evaluating microcapsule preparations, and they are also key technical issues that limit their wide application. The choice of wall material determines the quality of the prepared microcapsule product, and solving the high cost of preparation and the safety of wall material and auxiliary...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J13/02A01N25/28A01N43/60A01N43/42A01N43/90A01P7/04A01P13/00C09K15/08
Inventor 石国荣
Owner HUNAN AGRICULTURAL UNIV
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