High-temperature-resistant aerobic denitrification bacterial strain and application of same

An aerobic denitrifying bacteria and high temperature-resistant technology, applied in the field of environmental pollution bioremediation, can solve problems such as increasing the cost of denitrification, and achieve the effect of avoiding environmental incompatibility and large application potential.

Inactive Publication Date: 2015-08-12
NANJING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If room temperature aerobic denitrifying bacteria are used for biological denitrificati...

Method used

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  • High-temperature-resistant aerobic denitrification bacterial strain and application of same
  • High-temperature-resistant aerobic denitrification bacterial strain and application of same
  • High-temperature-resistant aerobic denitrification bacterial strain and application of same

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Example 1: Isolation of high temperature resistant aerobic denitrification strains

[0025] Collect water samples, pour them into sterilized sampling bottles, store them at low temperature, and bring them back to the laboratory immediately, take 100ml of the water, add 0.2g of sodium citrate, K 2 HPO 4 0.1g, KH 2 PO 4 0.1g, KNO 3 0.2g, MgSO 4 ·7H 2 O 0.02g, adjust the pH to 7.2-7.4, namely the denitrification medium. Put the denitrification medium and 1mm sterile glass beads into a 500ml Erlenmeyer flask that has been sterilized at 121°C for 20 minutes, seal it with sterilized gauze, and place it on a shaker at 30°C at 200rpm for enrichment of aerobic denitrifying bacteria . After cultivating for about 72 hours, the degerm-free suspension was inserted into fresh sterile denitrification medium (deionized water configuration) according to 10% inoculum size, and enriched by continuous passage for three times, and the culture conditions were the same as above. The e...

Embodiment 2

[0027] Example 2: The denitrification ability of high temperature resistant aerobic denitrifying bacteria at different ambient temperatures.

[0028]Collect surface water samples and measure the pH, temperature, total nitrogen, nitrate nitrogen, nitrite nitrogen, ammonia nitrogen and total carbon of the water samples. Inoculate the aerobic denitrifying bacteria Achromobacter sp.strain N1 obtained above into the liquid medium, shake and culture at 30°C and 200rpm until the logarithmic phase of growth, centrifuge at 12000rpm for 5min under sterile conditions, wash with sterilized 10mM sodium phosphate buffer After being resuspended twice, 10% of the inoculum was inoculated in the surface water sample of Meiliang Bay of Taihu Lake containing 20 mg / l nitrate nitrogen for experiment. The experimental conditions are: KNO 3 Adjust C / N=8 with sodium citrate, nitrate nitrogen content is 20mg / l, pH7.2-7.4. Respectively in different ambient temperatures: 4-60°C, 200rpm shaking culture ...

Embodiment 3

[0029] Example 3: The denitrification ability of aerobic denitrifying bacteria Achromobacter sp.strain N1 under high temperature environment.

[0030] Inoculate the aerobic denitrifying bacteria Achromobacter sp.strain N1 obtained above into the liquid medium, shake and culture at 30°C and 200rpm until the logarithmic phase of growth, centrifuge at 12000rpm for 5min under sterile conditions, wash with sterilized 10mM sodium phosphate buffer After twice and resuspension, 10% of the inoculum was inoculated in the surface water samples of Meiliang Bay of Taihu Lake containing different concentrations of nitrate nitrogen for experiments. The experimental conditions are: using KNO 3 Adjust C / N=8 with sodium citrate, make the concentration range of nitrate nitrogen 20-100 mg / l, pH 7.2-7.4, shake culture at 50° C. and 200 rpm for 12 hours. Sampling was carried out to determine the content of nitrate nitrogen and nitrite nitrogen in the water body, and the removal rate of nitrogen wa...

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Abstract

The invention belongs to the field of environmental pollution repairing and discloses a high-temperature-resistant aerobic denitrification bacterial strain, of which the classification name is Achromobacter sp. The bacterial strain has been preserved at China center for type culture collection at 2015 Jan.9 and has been assigned the accession number CCTCC NO: M2015025. The invention also discloses an application of the high-temperature-resistant aerobic denitrification bacteria. The strain grows well at 50 DEG C, has strong denitrification performance, is very less in accumulation of nitrite during denitrification and is free of secondary pollution. In the invention, the high-efficient aerobic denitrification bacteria are obtained through screening from a eutrophic lake water body, when being subjected to enlarged cultivation, the bacteria strain can be directly used for denitrification on lake water and can avoid environmental inadaptability of non-indigenous microorganisms. The bacteria strain has huge application potential and has a significant promoting effect on environmental protection for long term.

Description

technical field [0001] The invention belongs to the field of bioremediation of environmental pollution, specifically relates to a high-temperature-resistant aerobic denitrifying bacteria and its application, and is a selection and breeding of excellent microorganisms, thereby broadening the scope of treatment of eutrophic water bodies. Background technique [0002] In recent years, the large-scale discharge of industrial wastewater and the large-scale application of chemical fertilizers in agriculture have led to increasingly prominent water environmental problems caused by nitrogen pollution, among which nitrate pollution is particularly serious. Nitrate and its derivatives affect human health due to their pathogenic and carcinogenic effects. Therefore, increasing the treatment of nitrogen pollution in sewage, especially improving the treatment efficiency of nitrate nitrogen is an urgent problem to be solved in the current sewage treatment technology. [0003] Biological d...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01
CPCC02F3/34C12N1/205C12R2001/025
Inventor 肖琳陈旭张小龙杨玉杨柳燕
Owner NANJING UNIV
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