Method for reproducing verticillum lecanii and recovering vigor of verticillum lecanii by using larvas of argyrogramma agnata

Abstract

The invention provides a method for reproducing verticillum lecanii and recovering vigor of the verticillum lecanii by using larvas of argyrogramma agnata, and belongs to the biotechnology. The method comprises the following steps of feeding the argyrogramma agnata; culturing the verticillum lecanii; preparing conidium liquid of the verticillum lecanii; preparing the prepared conidium liquid into suspension liquid; uniformly spraying the suspension liquid to the larvas of the argyrogramma agnata until surfaces of the larvas are wet; placing the larvas in a sterile artificial climate room and culturing the larvas until hyphae grow on the larvas fully; collecting the larvas; dewatering and drying the larvas; and storing the larvas in a refrigerator of which the temperature is minus 95 DEG C to minus 78 DEG C. In a reproduction process of the verticillum lecanii reproduced by the method, the vigor and the infection ability of strain spores can be kept for a long time. Moreover, by the method, the vigor and the infection ability of strains of which the infection ability and the vigor are reduced severely can be recovered.

Description

technical field

[0001] The present invention belongs to biotechnology. Background technique

[0002] Verticillium lecanii (Zimm) is a common entomogenous fungus, belonging to the fungal phylum Deuteromycetes subphylum Hyphospora Hyphospora. The fungus has a wide geographical distribution and a wide range of hosts. It has good parasitic effects on agricultural pests such as aphids, whiteflies, scales, thrips and harmful mites. It is a relatively mature biological pesticide. In the previous research process, Verticillium lecanii often reproduced on a large scale in the form of solid or liquid medium. Verticillium lecanii KMZW-1 is a strain with high toxicity against fly insects screened by the patent applicant and obtained the patent (ZL200810233784.7). After 4 to 5 generations, the viability of the spores obviously began to decline. The study found that other Verticillium lecanii also had the problem of the ability to infect the host and the viability of the spores decrease...

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