Extraction and separation method of Agrocybe aegirit polysaccharides

A tea tree mushroom polysaccharide and separation method technology, which is applied in the field of extraction and separation of tea tree mushroom polysaccharides, can solve the problems of low yield of tea tree mushroom polysaccharides, low polysaccharide purity, and inability to improve the purity of tea tree mushroom polysaccharides, and achieves separation process improvement and extraction process Improved effect

Active Publication Date: 2015-08-19
开平市李氏实业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]Technical problem to be solved: tea tree mushroom polysaccharide is an active ingredient, and there are many separation and purification methods for tea tree mushroom polysaccharide, but most of the methods purify the polysaccharide The purity is low, and the yield of tea tree polysaccharides with a purity that can meet the needs is low, and the purity of tea tree polysaccharides cannot be improved while ensuring the yield, so a new extraction and separation method for tea tree polysaccharides is needed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] (1) Cut fresh tea tree mushrooms into slices and pre-cool them. After the tea tree mushrooms are frozen, perform freeze-drying. The freeze-drying temperature is -20°C and the freeze-drying time is 20h; (2) The tea tree mushrooms are sliced ​​after freeze-drying After pulverization, add 20wt% ethanol aqueous solution to the tea tree mushroom powder, the weight ratio of the ethanol aqueous solution to the tea tree mushroom is 7:1, and perform ultrasonic stirring extraction, the ultrasonic power is 300w, the stirring speed is 100rpm, and the extraction time is 4h , The extraction temperature is 45 ℃, after the extraction, the extract is centrifuged at an offline speed of 3500 rpm, and the supernatant is taken after centrifugation; (3) The supernatant is concentrated and concentrated to twice the weight of the tea tree mushroom; (4) ) The tea tree mushroom concentrate is filtered through a membrane, and the retentate is collected through a membrane with a molecular weight of ...

Embodiment 2

[0018] (1) Cut fresh tea tree mushrooms into slices and pre-cool them. After the tea tree mushrooms are frozen, perform freeze-drying. The freeze-drying temperature is -20°C and the freeze-drying time is 20h; (2) The tea tree mushrooms are sliced ​​after freeze-drying After pulverization, add 20wt% ethanol aqueous solution to the tea tree mushroom powder, the weight ratio of the ethanol solution to the tea tree mushroom is 10:1, perform ultrasonic stirring extraction, the ultrasonic power is 400w, the stirring speed is 100rpm, and the extraction time is 2h , The extraction temperature is 45 ℃, after the extraction, the extract is centrifuged at an offline speed of 3500 rpm, and the supernatant is taken after centrifugation; (3) The supernatant is concentrated and concentrated to twice the weight of the tea tree mushroom; (4) ) The tea tree mushroom concentrate is filtered through a membrane, and the retentate is collected through a membrane with a molecular weight of 1000; (5) t...

Embodiment 3

[0021] (1) Cut fresh tea tree mushrooms into slices and pre-cool them, freeze-dry the tea tree mushrooms after freezing, the freeze-drying temperature is -20℃, and the freeze-drying time is 12h; (2) After freeze-drying, the tea tree mushrooms are sliced After crushing, add 30wt% ethanol aqueous solution to the tea tree mushroom powder. The weight ratio of the ethanol aqueous solution to the tea tree mushroom is 5:1. Perform ultrasonic stirring extraction, the ultrasonic power is 200w, the stirring speed is 100rpm, and the extraction time is 5h , The extraction temperature is 45 ℃, after the extraction, the extract is centrifuged at an offline speed of 3500 rpm, and the supernatant is taken after centrifugation; (3) The supernatant is concentrated and concentrated to twice the weight of the tea tree mushroom; (4) ) The tea tree mushroom concentrate is filtered through a membrane, and the retentate is collected through a membrane with a molecular weight of 1000; (5) the retentate ...

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PUM

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Abstract

The invention belongs to the field of extraction and separation of polysaccharides, and discloses an extraction and separation method of Agrocybe aegirit polysaccharides. The extraction and separation method of Agrocybe aegirit polysaccharides comprises the following steps: 1, cutting fresh Agrocybe aegirit to form slices, pre-cooling the slices, and freeze-drying after the Agrocybe aegirit is frozen; 2, rapidly crushing the freeze-dried Agrocybe aegirit slices, adding an ethanol-water solution into the obtained Agrocybe aegirit powder, carrying out ultrasonic stirring extraction, centrifuging the obtained extraction liquid, and taking the obtained supernatant; 3, concentrating the supernatant until the weight of the obtained Agrocybe aegirit concentrate liquid is 2 times the weight of Agrocybe aegirit; 4, carrying out membrane filtration on the Agrocybe aegirit concentrate liquid through using a membrane with the molecular amount of 1000, and collecting the obtained trapped liquid; 5, allowing the trapped liquid to go through a membrane with the molecular amount of 200, and collecting the obtained new trapped liquid; 6, concentrating until no solvent exists; and 7, carrying out crystallizing purification on the obtained concentrate by using ethanol, filtering, and naturally drying to obtain the Agrocybe aegirit polysaccharides.

Description

technical field [0001] The invention belongs to the field of extraction and separation, and relates to a polysaccharide extraction and separation method, in particular to a tea tree mushroom polysaccharide extraction and separation method. Background technique [0002] Polysaccharide is a chain polymer formed by dehydration between monosaccharides to form glycosidic bonds, which are connected linearly or branched by glycosidic bonds. Generally, sugar chains with more than 20 sugar groups are called polysaccharides. In recent years, significant progress has been made in the determination of the structure and biological activity of carbohydrates. A large number of experimental facts have revealed that carbohydrates are important information molecules that participate in many physiological and pathological processes. Related research has penetrated into various fields of biology. Studies have shown that polysaccharides of traditional Chinese medicine have various functions su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 吴雷
Owner 开平市李氏实业发展有限公司
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