Preparation method of tuna myofibrillar protein-ZnO nanometer composite membrane

A myofibrillar protein and nanocomposite technology, which is applied in the field of nanocomposite membrane preparation, can solve the problems of not being effectively utilized and wasting fishery resources, etc., to inhibit lipid peroxidation and the growth of pathogenic microorganisms, and increase added value , the effect of reducing pollution

Inactive Publication Date: 2015-08-19
ZHEJIANG OCEAN UNIV
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AI Technical Summary

Problems solved by technology

During the processing of tuna fillets, etc., a large amount of leftovers such as minced mea

Method used

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Embodiment

[0017] Embodiment: The preparation method of tuna myofibrillar protein-ZnO nanocomposite film:

[0018] (1) Preparation of myofibrillar protein from minced tuna: Take minced tuna, add 0.05 mol / L Tris-Maleate buffer (pH 7.5) according to the ratio of solid to liquid 1 g: 10 mL, homogenize at 18000 r / min, centrifuge at 6000 r / min for 13 min, take the precipitate and Repeat the homogenization-centrifugation twice, the obtained precipitate was washed by adding 0.1 mol / L NaCl solution according to the solid-liquid ratio of 1 g:4 mL, and centrifuged at 9000 r / min for 15 min, and the obtained precipitate was again according to the solid-liquid ratio of 1 g:9 mL Add 0.1 mol / L NaCl solution to wash, filter with gauze, and centrifuge the filtrate to obtain a precipitate, which is the myofibrillar protein of tuna mince.

[0019] Preparation of Enteromorpha Antioxidants : After crushing the fresh Enteromorpha tissue, add ethyl acetate according to 1g:9 mL, ultrasonically extract at roo...

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Abstract

The invention discloses a preparation method of a tuna myofibrillar protein-ZnO nanometer composite membrane. A technical process comprises the steps that tuna ground meat myofibrillar proteins are prepared; a ZnO nanometer solution is prepared; then, the prepared myofibrillar proteins are dissolved in double distilled water; in addition, ZnO nanometer particles, enteromorpha antioxidant agents and plasticizers are sequentially added for preparing a membrane forming solution; finally, the successfully prepared membrane forming solution is poured onto a polyvinyl chloride crystal board for scrapping, drying and membrane uncovering, and the myofibrillar protein-ZnO nanometer composite membrane is prepared. The preparation method has the advantages that tuna ground meat is used as major raw materials, a new path is opened up for the high-valued utilization of processing leftovers of tunas, and the prepared myofibrillar protein-ZnO nanometer composite membrane has the obvious characteristics of anti-oxidation, bacteriostasis and the like.

Description

technical field [0001] The invention relates to a preparation method of a nanocomposite film, in particular to a preparation method of a tuna myofibrillar protein-ZnO nanocomposite film. Background technique [0002] Myofibrillar protein accounts for more than 50% of fish protein, and is the most biologically functional structural protein group in fish. Its structure, gel and rheological properties have an important impact on the quality of fish products. In recent years, the research on myofibrillar protein has found that it has good film-forming properties, which has laid a theoretical foundation for its application in the field of biofilm preparation. [0003] Tuna is an important pelagic commercial food fish with an annual catch of more than 6 million tons. During the processing of tuna and fish fillets, a large amount of leftovers such as minced meat are produced, which are not effectively utilized and precious fishery resources are wasted. [0004] Based on this, the...

Claims

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Application Information

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IPC IPC(8): C08J5/18C08L89/00C08K3/22C08K5/053C08K5/1535
Inventor 迟长凤王斌陈荫孙坤来
Owner ZHEJIANG OCEAN UNIV
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