Preparation method of tuna myofibrillar protein-ZnO nanometer composite membrane
A myofibrillar protein and nanocomposite technology, which is applied in the field of nanocomposite membrane preparation, can solve the problems of not being effectively utilized and wasting fishery resources, etc., to inhibit lipid peroxidation and the growth of pathogenic microorganisms, and increase added value , the effect of reducing pollution
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[0017] Embodiment: The preparation method of tuna myofibrillar protein-ZnO nanocomposite film:
[0018] (1) Preparation of myofibrillar protein from minced tuna: Take minced tuna, add 0.05 mol / L Tris-Maleate buffer (pH 7.5) according to the ratio of solid to liquid 1 g: 10 mL, homogenize at 18000 r / min, centrifuge at 6000 r / min for 13 min, take the precipitate and Repeat the homogenization-centrifugation twice, the obtained precipitate was washed by adding 0.1 mol / L NaCl solution according to the solid-liquid ratio of 1 g:4 mL, and centrifuged at 9000 r / min for 15 min, and the obtained precipitate was again according to the solid-liquid ratio of 1 g:9 mL Add 0.1 mol / L NaCl solution to wash, filter with gauze, and centrifuge the filtrate to obtain a precipitate, which is the myofibrillar protein of tuna mince.
[0019] Preparation of Enteromorpha Antioxidants : After crushing the fresh Enteromorpha tissue, add ethyl acetate according to 1g:9 mL, ultrasonically extract at roo...
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