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An antibacterial traditional Chinese medicine microbial fermentation preparation and a preparation method and application thereof

A technology of microbial fermentation and traditional Chinese medicine, applied in antibacterial drugs, drug combinations, pharmaceutical formulas, etc., can solve the problems of co-fermentation of Lactobacillus acidophilus and Pediococcus pentosaceae, etc., and achieve good clinical application prospects and small side effects Effect

Inactive Publication Date: 2015-08-26
CHENGDU QIANKUN VETERINARY PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There is no report on co-fermenting Chinese medicine with Lactobacillus acidophilus and Pediococcus pentosacea to prepare Chinese medicine microbial fermentation agent

Method used

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  • An antibacterial traditional Chinese medicine microbial fermentation preparation and a preparation method and application thereof
  • An antibacterial traditional Chinese medicine microbial fermentation preparation and a preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0026] Embodiment 1 The preparation of Chinese medicine microbial fermentation preparation of the present invention

[0027] 1.1 Experimental materials

[0028] 1.1.1 Bacterial strain: Pediococcus pentosacea (preservation number 21862), Lactobacillus acidophilus (accession number 20710), Escherichia coli K88, K99, 987P standard strains, and Staphylococcus aureus were purchased from China Industrial Microorganism Culture Collection Center (CICC).

[0029] 1.1.2 Medium. ① MRS liquid medium: used to cultivate Lactobacillus acidophilus. Peptone 10g, beef extract 10g, yeast extract 5g, K2HP042g, sodium acetate 5g, ammonium citrate 2g, glucose 20g, Tween-801mL, magnesium sulfate 0.58g, manganese sulfate 0.25g, add distilled water to 1000ml, adjust pH to 6.0. ② MRS solid medium: used to cultivate Lactobacillus acidophilus. Add 1.5%-2.0% agar to the prepared ship's liquid medium. ③ Beef extract peptone medium: Escherichia coli and Staphylococcus aureus. Add 0.3g of beef extra...

Embodiment 2

[0034] Embodiment 2 The preparation of Chinese medicine microbial fermentation preparation of the present invention

[0035] 1.1 Experimental materials

[0036] 1.1.1 Bacterial strain: Pediococcus pentosacea (preservation number 21862), Lactobacillus acidophilus (accession number 20710), Escherichia coli K88, K99, 987P standard strains, and Staphylococcus aureus were purchased from China Industrial Microorganism Culture Collection Center (CICC).

[0037] 1.1.2 Medium. ① MRS liquid medium: used to cultivate Lactobacillus acidophilus. Peptone 10g, beef extract 10g, yeast extract 5g, K2HP042g, sodium acetate 5g, ammonium citrate 2g, glucose 20g, Tween-801mL, magnesium sulfate 0.58g, manganese sulfate 0.25g, add distilled water to 1000ml, adjust pH to 6.0. ② MRS solid medium: used to cultivate Lactobacillus acidophilus. Add 1.5%-2.0% agar to the prepared ship's liquid medium. ③ Beef extract peptone medium: Escherichia coli and Staphylococcus aureus. Add 0.3g of beef extra...

Embodiment 3

[0042] Embodiment 3 The preparation of Chinese medicine microbial fermentation preparation of the present invention

[0043] 1.1 Experimental materials

[0044] 1.1.1 Bacterial strain: Pediococcus pentosacea (preservation number 21862), Lactobacillus acidophilus (accession number 20710), Escherichia coli K88, K99, 987P standard strains, and Staphylococcus aureus were purchased from China Industrial Microorganism Culture Collection Center (CICC).

[0045]1.1.2 Medium. ① MRS liquid medium: used to cultivate Lactobacillus acidophilus. Peptone 10g, beef extract 10g, yeast extract 5g, K2HP042g, sodium acetate 5g, ammonium citrate 2g, glucose 20g, Tween-801mL, magnesium sulfate 0.58g, manganese sulfate 0.25g, add distilled water to 1000ml, adjust pH to 6.0. ② MRS solid medium: used to cultivate Lactobacillus acidophilus. Add 1.5%-2.0% agar to the prepared ship's liquid medium. ③ Beef extract peptone medium: Escherichia coli and Staphylococcus aureus. Add 0.3g of beef extrac...

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Abstract

The present invention provides an antimicrobial traditional Chinese medicine microbial fermentation preparation which uses traditional Chinese medicines as raw materials and is fermented by lactobacillus acidophilus and pediococcus pentosaceus collaboratively; the traditional Chinese medicines consist of portulaca oleracea, forsythia suspensa or phellodendron chinense bark. The present invention also provides a preparation method and application of the above antimicrobial traditional Chinese medicine microbial fermentation preparation. In summary, the traditional Chinese medicine microbial fermentation preparation can be more effective in treating piglets' diarrhea than antibiotics, has low side effects, can replace the use of antibiotics. Meanwhile, the traditional Chinese medicine microbial fermentation preparation has more significant inhibition effects for clinical pathogenic bacteria Escherichia coli and Staphylococcus aureus than the separate use of bacteria and traditional Chinese medicines, and has excellent clinical application prospect.

Description

technical field [0001] The invention relates to an antibacterial traditional Chinese medicine microbial fermentation preparation, a preparation method and application thereof. Background technique [0002] At present, bacterial flora imbalance caused by abuse of antibiotics has become a common phenomenon in veterinary clinics. In order to solve the problem of antibiotic abuse, domestic and foreign scholars have developed many feasible measures, among which microecological preparations are considered to be the most promising products to replace antibiotics. Chinese herbal medicine has the advantages of a long history, rich resources, comprehensive functions, less toxic and side effects, and no residue in my country. Microorganisms have a very rich enzyme system and have a strong ability to decompose and transform substances. Fermenting traditional Chinese medicine with microorganisms may biotransform the chemical components of traditional Chinese medicine, produce new compoun...

Claims

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Application Information

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IPC IPC(8): A23K1/14A23K1/00A61K36/756A61P31/04A61P1/12
Inventor 余琴唐华侨房春林李超李犹平杨海涵丁晓刚
Owner CHENGDU QIANKUN VETERINARY PHARMA
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