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Method for preparing β-sitosterol linoleate from Solanum nigrum plants

A technology of sitosterol linoleate and nightshade, applied in the directions of steroids, organic chemistry, etc., can solve the problem of not extracting β-sitosterol linoleate, etc., and achieves easy large-scale industrial production and practical production. Strong performance, improve the effect of effective utilization

Active Publication Date: 2017-06-13
上交(淮安)新材料技术创新中心有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] After searching the existing technical literature, it is found that there is no report on the extraction of β-sitosterol linoleate from the plant Solanum nigrum

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Wash and dry Solanum nigrum, dry at 40°C, crush and pass through a 50-mesh sieve, weigh 100g of powder, soak in absolute ethanol with 10 times the weight of the powder at room temperature for 120h, filter, and depressurize the filtrate at 40°C Concentrate to obtain 15.1 g of the concentrate, then dissolve the concentrate with 1000 ml of deionized water, extract 3 times with an equal volume of petroleum ether, concentrate the petroleum ether phase obtained by extraction under reduced pressure, and use a 200-300 mesh silica gel column to The petroleum ether-ethyl acetate system is used as the mobile phase to increase the proportion of ethyl acetate for gradient elution (for example, the ratio of 190:1, 150:1, 100:1, 50:1, 25:1, 20:1, 15:1, 10: 1, 5:1, 3:1, 2:1, 1:1, 10:1, 50:1; of course, other ratios can also be used in other embodiments, which can be determined according to general principles and actual conditions). The target product was collected by TLC detection, the...

Embodiment 2

[0028] Wash and dry Solanum solanum, dry at 50°C, crush and pass through a 30-mesh sieve, weigh 200g of powder, soak in absolute ethanol with 38 times the powder mass at room temperature for 24h, filter, and depressurize the filtrate at 40°C Concentrate to obtain 25.1 g of the concentrate, then dissolve the concentrate with 6000 ml of deionized water, then extract 3 times with 5 times the volume of petroleum ether, concentrate the extracted petroleum ether phase under reduced pressure, and use a 200-300 mesh silica gel column, Gradient elution was carried out by using the petroleum ether-ethyl acetate system as the mobile phase and increasing the proportion of ethyl acetate. The target product was collected by TLC detection, the components containing the target substance were collected, concentrated and the solvent was evaporated to dryness, and then the obtained concentrate was further purified through a gel column Sephadex LH-20, using a chloroform-methanol system as the mobi...

Embodiment 3

[0030] Wash and dry Solanum solanum, dry at 45°C, crush and pass through a 10-mesh sieve, weigh 100g of powder, soak in absolute ethanol 20 times the weight of the powder at room temperature for 168h, filter, and depressurize the filtrate at 40°C Concentrate to obtain 17.5 g of the concentrate, then dissolve the concentrate with 300 ml of deionized water, then extract 3 times with 3 times the volume of petroleum ether, concentrate the extracted petroleum ether phase under reduced pressure, and use a 200-300 mesh silica gel column, Gradient elution was carried out by using the petroleum ether-ethyl acetate system as the mobile phase and increasing the proportion of ethyl acetate. The target product was collected by TLC detection, the components containing the target substance were collected, concentrated and the solvent was evaporated to dryness, and then the obtained concentrate was further purified through a gel column Sephadex LH-20, using a chloroform-methanol system as the ...

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PUM

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Abstract

The invention provides a method for preparing beta-sitosterol linoleate from a solanum nigrum plant. The method comprises the following steps: step 1, subjecting solanum nigrum to cleaning and air drying and then carrying out drying and crushing to obtain powder; step 2, soaking and extracting the powder with ethanol at normal temperature, then carrying out filtering and subjecting obtained filtrate to pressure reduced vacuum concentration so as to obtain a concentrate of extract; step 3, dissolving the concentrate of extract obtained in the previous step and carrying out extraction with petroleum ether; step 4, subjecting a petroleum ether phase obtained after extraction to pressure reduced vacuum concentration and carrying out gradient elution with a silica gel column; step 5, after passing through the silica gel column, collecting a target product and carrying out pressure reduced vacuum concentration; and step 6, further purifying a concentrate obtained in the step 5 with a gel column in which a chloroform-methanol system is used as a mobile phase, thereby eventually obtaining light yellow grease, i.e., beta-sitosterol linoleate. The method is simple to operate, has low cost and prepares high-purity beta-sitosterol linoleate.

Description

technical field [0001] The invention relates to a preparation method in the field of agricultural technology, in particular to a method for preparing β-sitosterol linoleate from plant Solanum nigrum. Background technique [0002] Nightshade (Solanum Nigrum L.) is an annual plant of the genus Solanum. It is a widely distributed weed. It mostly grows on roadsides and wastelands. It likes warm and humid climates. It is therefore very easy to grow and has a large biomass. Its whole plant can be used for medicine, and it is often used to treat sores, carbuncles, tumors, skin eczema, and dysuria. At present, there are many studies on the chemical components of Solanum nigrum at home and abroad. It has been reported that Solanum nigrum contains alkaloids such as solanigrine and solasonine, as well as saponins, vitamin C, and resins. [0003] β-sitosterol linoleate is an important phytosterol ester, which has been reported to have strong antioxidant and bactericidal effects. At p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J9/00
CPCC07J9/00
Inventor 代光辉陈义娟
Owner 上交(淮安)新材料技术创新中心有限公司
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