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A kind of cultivation substrate for banana tissue culture seedlings and its preparation method and application

A technology of cultivation substrate and tissue culture seedlings, which is applied in the field of banana tissue culture cultivation substrate and its preparation, can solve the problems of banana pseudostem pollution, banana soil environment damage, etc., and achieve optimization of metabolic activity, simple and feasible operation, and avoid pollution Effect

Active Publication Date: 2017-07-14
POMOLOGY RES INST GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problems of banana soil environmental damage and banana pseudostem pollution, to provide a method that can reduce banana pseudostem pollution, efficiently improve the soil environment, optimize the metabolic activity of banana seedling soil microbial communities, and promote the growth of banana seedlings, Environmentally friendly and easy-to-operate banana tissue culture medium and its preparation method and application

Method used

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  • A kind of cultivation substrate for banana tissue culture seedlings and its preparation method and application
  • A kind of cultivation substrate for banana tissue culture seedlings and its preparation method and application
  • A kind of cultivation substrate for banana tissue culture seedlings and its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: the preparation of banana pseudostem biochar

[0026] 1.1 Preparation of banana pseudostem biochar

[0027] Cut the banana pseudostem into pieces and air-dry it naturally to reduce the water content to 15%, and reduce the moisture content of the banana pseudostem used for ignition to 8%; use a pulverizer to crush the above-mentioned air-dried banana pseudostem into a diameter of 2-10mm particle. Put the banana pseudostem for ignition under the combustion leaflet in the combined carbonization furnace, and ignite it with an open flame; then add the air-dried banana pseudostem into the furnace body, and the material layer is 60cm higher than the burner. Feed once every 1 hour, the thickness is 10-30cm, and the oxygen supply in the furnace body is manually controlled by the thickness of the material layer. After loading the banana pseudo-stem material for the last time, buckle the top cover on the top of the furnace body. When a large amount of steam overflo...

Embodiment 2

[0054] Embodiment 2: prepare banana tissue culture seedling cultivation substrate

[0055] 2.1 Mix peat soil and coir peat at a volume ratio of 1:2 to prepare nutritious soil, add banana pseudostem biochar to make it account for 1% of the total mass of the banana tissue culture medium, and mix evenly to obtain 1% banana group Cultivation substrate for seedlings.

[0056] 2.2 Mix peat soil and coir peat at a volume ratio of 1:2 to prepare nutritious soil, add banana pseudostem biochar to make it account for 2% of the total mass of the banana tissue culture medium, and mix evenly to obtain 2% banana group Cultivation substrate for seedlings.

[0057] 2.3 Mix peat soil and coir peat at a volume ratio of 1:2 to prepare nutritious soil, add banana pseudostem biochar to make it account for 3% of the total mass of the banana tissue culture medium, and mix evenly to obtain 3% banana group Cultivation substrate for seedlings.

Embodiment 3

[0059] 3.1 Determination of biomass of banana plants in each treatment group

[0060] Under normal cultivation and management conditions, the control group (CK, the cultivation substrate of the control group was uniformly prepared by mixing peat soil and coconut bran at a volume ratio of 1:2) and the treatment group (1%, 2%, 3 %, its cultivation substrate is respectively 1% banana tissue culture seedling cultivation substrate of embodiment 2, 2% banana tissue culture cultivation substrate and 3% banana tissue culture cultivation substrate) the banana root when growing to 3 months , stems, leaves (4 banana plants in each group), 105 ℃ killing, 80 ℃ drying to constant weight and weighing its dry weight, the results show that the biomass content of banana plants in the treatment group was significantly higher than that of the control group. The results are shown in Table 5.

[0061] Banana biomass under different treatments in table 5

[0062]

[0063] Among them, CK means t...

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Abstract

The invention provides a banana tissue culture seed culture substrate as well as a preparation method and application thereof. Banana cauloid biological carbon is prepared by the steps of chopping banana cauloid, naturally drying with air until the moisture content is reduced to be lower than 15%, crushing the banana cauloid biological carbon into particles with the diameter of 2mm-10mm, and machining in a carbonization furnace. The banana tissue culture seed culture substrate is formed by uniformly mixing 1%-3% of the banana cauloid biological carbon by mass and the balance of nutrient soil. The banana cauloid biological carbon is applied and has obvious optimization effect on the metabolic activity of banana seedling soil micro-flora, and the metabolic activity of a lot of bacteria and the biological carbon bring in a lot of nutrient elements; and the fertility condition of the soil is improved and the growth of banana tissue culture seedlings is remarkably facilitated. The banana cauloid biological carbon has a very good application effect in banana culture and has a wide prospect in the culture application of bananas and other plants.

Description

technical field [0001] The invention belongs to the field of preparation and application of biomass fertilizers in agriculture, and in particular relates to a cultivation substrate for banana tissue culture seedlings, a preparation method and application thereof. Background technique [0002] As a major economic fruit, bananas are widely planted in tropical and subtropical regions, and their traditional cultivation methods rely heavily on a large amount of chemical fertilizers, which often lead to problems such as soil structure damage, soil compaction, organic matter content decline, and microbial community activity in banana orchards. . On the other hand, stacking a large number of banana pseudostems in the field after harvest is likely to cause pollution, and the accumulation of banana pseudostems with germs is likely to cause the spread and spread of germs such as Fusarium wilt. [0003] Biochar refers to the pyrolysis of biomass into carbon-rich solid matter under anae...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 董涛李航王明元易干军高慧君李鹏
Owner POMOLOGY RES INST GUANGDONG ACADEMY OF AGRI SCI