Cell-assembled small intestinal submucosa biomimetic composite engineered bone and preparation method thereof

Abstract

The invention discloses a cell assembling small-intestinal submucosa bionic composite engineering bone; the cell assembling small-intestinal submucosa bionic composite engineering bone has higher mechanical strength; the elasticity modulus is 350 MPa and 100 MPa respectively in dry and wet states; better tissue support can be provided in an implant in the initial stage; the tissue engineering bone is very close to a mineralized structure and distribution in a natural bone; the tissue engineering bone has a micro-structure highly similar to the natural bone; after being implanted, the tissue engineering bone is beneficial to well identifying and accelerating osteanagenesis; a preparation method of the bionic composite engineering bone disclosed by the invention is a novel combination inducing osteoblast differentiating and mineralizing method; osteoblast on SIS (Styrene Isoprene Styrene) is stimulated through calcium ion and icariin combined culture solution; a lot of in-situ mineralizing and osteogenic differentiation on the SIS material are carried out; simultaneously, inhibition of calcium ion to important matrix protein OCN in the later differentiation period can be avoided; the bone conductivity of the engineering bone is enhanced; a lot of hydroxyapatite crystallization and osteoblastic-like cell epimatrix deposition on the SIS material are finally realized; and furthermore, compared with SIS self, the mechanical strength is greatly increased.

Description

technical field [0001] The invention relates to the technical field of using tissue engineering technology to construct artificial tissues / organs in biomedical engineering, in particular to a cell-assembled small intestinal submucosa bionic composite engineering bone and a preparation method thereof. Background technique [0002] About 3.5 million people in my country have bone defects due to different reasons every year, and about 1.5 million bone surgery transplants are performed. The demand for replacement bones is very large, especially for replacement bones for large bone injuries. With the rapid development of tissue engineering technology, constructing osteoconductive and osteoinductive artificial substitute bone in vitro is an important way to solve this demand. Skeleton materials play a vital role in tissue engineering bone construction. Compared with other synthetic materials, natural extracellular matrix (ECM) materials have the following characteristics: 1. Excel...

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Problems solved by technology

Since SIS itself is taken from soft tissue, it has the defect of insufficient mechanical strength as a bone tissue engineering framework material. In order to provide better support for the SIS framework material in the initial stage of implantation, its mechanical strength needs to be increased.

[0003] The inorganic component of natural bone is mainly composed of hydroxyapatite. Directly mixing SIS with hydroxyapatite as artificial bone can improve the mechanical strength and make the composition of the matrix material close to that of natural bone, but this kind of mixing only realizes the macroscopic The components imitate the composition of natural bone, but in terms of microstructure, the spatial distribution and combination of calcium phosphate molecules and other extracellular matrix components are still quite different from those of natural bone.

[0004] The osteoinductive factors needed in bone tissue engineering have always been a research hotspot. Although BMP-2 protein is the most effective osteogenic induction factor widely recognized and adopted, it is very expensive, and the BMP gene introduction method still exists. Certain safety issues, so its clinical use is still restricted by cost and safety; at the same time, BMP-2 and other protein drugs have problems of poor stability and short half-life in vivo, correspondingly, the requirements for drug release systems are very high

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