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Preparation method of porous heptapeptide self-assembled micro-ball
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A self-assembly and self-assembly solution technology, applied in the preparation of microspheres, microcapsule preparations, etc., can solve the problems of limited application and lack of excellent performance of inorganic functional materials, and achieve simple operation, excellent biocompatibility, and reaction conditions. mild effect
Inactive Publication Date: 2015-09-02
DONGHUA UNIV
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[0003] However, peptide self-assembly does not have the excellent properties of many inorganic functional materials (such as electronics, optics, catalysis, etc.), which largely limits its application.
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Embodiment 1
[0028] (1) Preparation of 7AAP solution: Dissolve 0.010g of 7AAP in an EP tube containing 100 μL of 1,1,1,3,3,3,-hexafluoro-2-propanol at room temperature, and filter through a 0.22 μm hydrophobic filter;
[0029] (2) Prepare PTA solution; dissolve 0.0065g of PTA in an EP tube filled with 50ml of distilled water, ultrasonicate for 60s; adjust the pH to 2;
[0030] (3) Preparation of 7AAP / PTA composite hydrogel microspheres: pipette 1.0 mL of PTA solution into the EP tube containing 10.0 μL of 7AAP solution, shake slightly by hand, and let stand for 4 hours;
[0031] (4) Put the self-assembly reaction solution in (3) into a dialysis bag, dialyze in neutral deionized water for 12 hours, take it out into an EP tube and let it stand for 30 days, then freeze-dry it.
Embodiment 2
[0033] (1) Preparation of 7AAP solution: Dissolve 0.020g of 7AAP in an EP tube containing 100 μL of 1,1,1,3,3,3,-hexafluoro-2-propanol at room temperature, and filter through a 0.22 μm hydrophobic filter;
[0034] (2) Prepare PTA solution; dissolve 0.0065g of PTA in an EP tube filled with 50mL of distilled water, ultrasonicate for 60s; adjust the pH to 2;
[0035] (3) Preparation of 7AAP / PTA composite hydrogel microspheres: pipette 1.0 mL of PTA solution into the EP tube containing 10.0 μL of 7AAP solution, shake slightly by hand, and let stand for 4 hours;
[0036] (4) Put the self-assembly reaction solution in (3) into a dialysis bag, dialyze in neutral deionized water for 12 hours, take it out into an EP tube and let it stand for 30 days, then freeze-dry it.
Embodiment 3
[0038] (1) Preparation of 7AAP solution: Dissolve 0.030g of 7AAP in an EP tube containing 100 μL of 1,1,1,3,3,3,-hexafluoro-2-propanol at room temperature, and filter through a 0.22 μm hydrophobic filter;
[0039] (2) Prepare PTA solution; dissolve 0.0065g of PTA in an EP tube filled with 50mL of distilled water, ultrasonicate for 60s; adjust the pH to 2;
[0040] (3) Preparation of 7AAP / PTA composite hydrogel microspheres: pipette 1.0 mL of PTA solution into the EP tube containing 10.0 μL of 7AAP solution, shake slightly by hand, and let stand for 4 hours;
[0041] (4) Put the self-assembly reaction solution in (3) into a dialysis bag, dialyze in neutral deionized water for 12 hours, take it out into an EP tube and let it stand for 30 days, then freeze-dry it.
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Abstract
The invention relates to a preparation method of a porous heptapeptide self-assembled micro-ball. The preparation method comprises the following steps: (1) dissolving heptapeptide 7AAP into a 1,1,1,3,3,3-hexafluoro-2-propanol solution, and dissolving phosphotungstic acid PTA into distilled water, and carrying out ultrasonic treatment; (2) at a room temperature, putting the PTA water solution in the step (1) into the 7AAP in the step (1), vibrating and standing to obtain a self-assembled solution; and (3) adding the self-assembled solution in the step (2) into a dialysis bag, putting the dialysis bag into de-ionized water to be dialyzed, taking out, standing for more than 30 days, and freezing and drying to obtain the porous 7AAP / PTA self-assembled micro-ball. The preparation method is easy and convenient to operate and has moderate reaction conditions; the used raw materials are free of toxin and pollution, and have good biocompatibility; and the obtained porous 7AAP / PTA micro-ball has the relatively uniform grain diameter and the relatively regular shape, and is internally provided with a cavity structure.
Description
technical field [0001] The invention belongs to the field of polypeptide self-assembly, in particular to a method for preparing porous heptapeptide self-assembly microspheres. Background technique [0002] Polypeptides are biologically active macromolecules inherent in living organisms, which are composed of a variety of amino acids bound by peptide bonds in a certain order. Due to the different chemical structures of the amino acid residues on the polypeptide chain segment, the polypeptide can be effectively realized by utilizing the hydrogen bond interaction between peptide bonds and the hydrogen bond interaction, electrostatic interaction, hydrophobic interaction and π-π stacking interaction between amino acid residues. Molecular self-assembly. Peptide self-assembly refers to a class of molecular aggregates or supramolecular structures with clear structure, stable structure, and certain physical and chemical properties formed by the spontaneous combination of amino acid ...
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