Method for extracting betulinic acid by subcritical water

A technology of betulinic acid and subcritical water, applied in the direction of steroids, organic chemistry, etc., can solve the problem that there is no betulinic acid, and achieve the effects of high yield, simple process and short time

Inactive Publication Date: 2015-09-02
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Method for extracting betulinic acid by subcritical water
  • Method for extracting betulinic acid by subcritical water

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Embodiment 1

[0038] A method for extracting betulinic acid with subcritical water of the present invention, specifically comprises the following steps:

[0039] (1) Crush birch bark to 40 mesh to obtain birch bark powder.

[0040] (2) Put the white birch bark powder obtained in step (1) into a subcritical water extraction device, use water as the extraction liquid to extract the white birch bark powder, the extraction pressure is 1.0MPa, the extraction temperature is 150°C, and the water The liquid-solid ratio to birch bark was 20mL / g, the extraction time was 15min, and the extraction was performed twice to obtain the mixed solution A.

[0041] The subcritical water extraction device is a TGYF-A stainless steel high-pressure extractor.

[0042] (3) Put the mixture A into the centrifuge tube of the centrifuge, centrifuge at a speed of 5000r / min for 5min, then remove the centrifuge tube to obtain the extract A and residue A.

[0043] (4) Add absolute ethanol to residue A, the liquid-solid ...

Embodiment 2

[0052] A method for extracting betulinic acid with subcritical water of the present invention, specifically comprises the following steps:

[0053] (1) Crush birch bark to 80 mesh to obtain birch bark powder.

[0054] (2) Put the white birch bark powder obtained in step (1) into a subcritical water extraction device, and use water as the extraction liquid to extract the white birch bark powder. The extraction pressure is 0.5MPa, the extraction temperature is 180°C, and the water The liquid-solid ratio to birch bark was 30mL / g, the extraction time was 30min, and the extraction was performed once to obtain the mixed solution A.

[0055] The subcritical water extraction device is a TGYF-A stainless steel high-pressure extractor.

[0056] (3) Put the mixture A into the centrifuge tube of the centrifuge, centrifuge at 4000r / min for 10min, then remove the centrifuge tube to obtain the extract A and residue A.

[0057] (4) Add absolute ethanol to residue A, the liquid-solid ratio o...

Embodiment 3

[0065] A method for extracting betulinic acid with subcritical water of the present invention, specifically comprises the following steps:

[0066] (1) Crush birch bark to 40 mesh to obtain birch bark powder.

[0067] (2) Put the white birch bark powder obtained in step (1) into a subcritical water extraction device, use water as the extraction liquid to extract the white birch bark powder, the extraction pressure is 2.0MPa, the extraction temperature is 130°C, and the water The liquid-solid ratio to birch bark was 40mL / g, the extraction time was 15min, and the extraction was performed 3 times to obtain the mixed solution A.

[0068] The subcritical water extraction device is a TGYF-A stainless steel high-pressure extractor.

[0069] (3) Put the mixture A into the centrifuge tube of the centrifuge, centrifuge at a speed of 3000r / min for 15min, then remove the centrifuge tube to obtain the extract A and residue A.

[0070] (4) Add absolute ethanol to residue A, the liquid-sol...

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Abstract

The invention provides a method for extracting betulinic acid by subcritical water and belongs to the technical field of extraction and separation of active ingredients of natural products. The method includes the steps of smashing materials, placing the smashed materials in a subcritical water extracting device, extracting raw material powder by taking water as extracting liquid, adding sodium hydroxide in extract after the extractive is subjected to alcohol washing, dissolving out crude betulinol, adding the sodium hydroxide in alkali liquor to dissolve out rough betulinic acid, and recrystallizing to obtain the betulinic acid. By means of utilizing an advanced subcritical water extracting technology, the method for extracting the betulinic acid by the subcritical water has the advantages of high extracting efficiency, short extracting time, no pollution, high betulinol and betulinic acid production rate and the like, and industrial application of the method is easy to achieve.

Description

technical field [0001] The invention relates to a method for extracting betulinic acid with subcritical water, and belongs to the technical field of extraction and separation of natural product active components. Background technique [0002] Betulinic acid, also known as betulinic acid, is a pentacyclic triterpenoid compound that is widely distributed in a variety of plants, such as jujube seed in Rhamnaceae, birch bark in Betaceae, saponins in Fabaceae, Rosaceae Papaya, Araliaceae Acanthopanax, Euphorbiaceae Chongyang wood, Malvaceae hibiscus and so on. Betulinic acid has significant anti-tumor and anti-HIV activities, and can selectively kill human melanoma cells without killing healthy cells. It is a class of promising drug lead compounds. [0003] At present, the common extraction method of betulinic acid is solvent extraction, such as chloroform, benzene, ethanol, methanol and other solvents. In the patent CN101357934A, the inventor used ethanol to extract betulinic ...

Claims

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Application Information

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IPC IPC(8): C07J63/00
CPCC07J63/008
Inventor 刘静雷建都王璐莹陈盼姚文佳王佳
Owner BEIJING FORESTRY UNIVERSITY
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