A kind of making method of the thread plug that mouse mcao model is used

A production method and suture technology, applied in the field of life science, can solve the problems of difficult observation and research for a long time, no expansion effect, incomplete infarction, etc., and achieve stable cerebral ischemia, reduce mechanical damage, and reduce vascular stimulation. Effect

Inactive Publication Date: 2016-09-28
THE FIRST AFFILIATED HOSPITAL OF HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Commonly used glue coating methods include paraffin-coated, silica-gel-coated, polyhexenol thread, etc. Among them, the paraffin-coated thread is easy to fall off; although the infarct area is relatively stable with the silicone-coated thread plug, it has no expansion effect, making the infarction incomplete , and the mortality rate is high, and it is not easy to observe and study for a long time; the polyhexenol thread plug is not hard enough and will swell when encountering blood, making the thread entry difficult

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: A method for making a thread plug for a mouse MCAO model, through selection and preparation of materials, coating of the head end and marking after coating, finally obtain a thread plug suitable for making a mouse MCAO model, Its specific production method is as follows:

[0024] 1. Selection and preparation of production materials

[0025] 1.1. Thread bolt material

[0026] After the polytetrafluoroethylene resin is drawn into a filament with a diameter of 0.065mm at 300°C, it is coated with titanium dioxide on the surface of the filament at 350°C to obtain the original thread for making the thread plug. The natural tension of the original wire is 1.96N, and the traction tension is 1.67±0.78 N;

[0027] 1.2. The coating material of the head end

[0028] The coating material used to coat the head end of the thread bolt is a transparent polymer antibacterial material, which is composed of 15% silicon emulsion, 34% polyvinylpyrrolidone, 23% polyvinyl alco...

Embodiment 2

[0039] Embodiment 2: A method for making a thread plug for a mouse MCAO model. Through the selection and preparation of materials, coating of the head end and marking after coating, a thread plug suitable for the mouse MCAO model is finally obtained. The specific production method is as follows:

[0040] 1. Selection and preparation of production materials

[0041] 1.1. Thread bolt material

[0042] After the polytetrafluoroethylene resin is drawn into a filament with a diameter of 0.1mm at 300°C, it is coated with titanium dioxide on the surface of the filament at 350°C to obtain the original thread for making the thread plug. The natural tension of the original wire is 1.88N, and the traction tension is 1.71±0.82 N;

[0043] 1.2. The coating material of the head end

[0044] The coating material used to coat the head end of the thread bolt is a transparent polymer antibacterial material, which is composed of 15% silicon emulsion, 34% polyvinylpyrrolidone, 23% polyvinyl alco...

Embodiment 3

[0055] Embodiment 3: A method for making a thread plug for a mouse MCAO model. Through the selection and preparation of materials, coating of the head end and marking after coating, a thread plug suitable for the mouse MCAO model is finally obtained. The specific production method is as follows:

[0056] 1. Selection and preparation of production materials

[0057] 1.1. Thread bolt material

[0058] After the polytetrafluoroethylene resin is drawn into a filament with a diameter of 0.08mm at 300°C, it is coated with titanium dioxide on the surface of the filament at 350°C to obtain the original thread for making the thread plug. The natural tension of the original wire is 1.92N, and the traction tension is 1.66±0.9 N;

[0059] 1.2. The coating material of the head end

[0060] The coating material used to coat the head end of the thread bolt is a transparent polymer antibacterial material, which is composed of 15% silicon emulsion, 34% polyvinylpyrrolidone, 23% polyvinyl al...

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Abstract

Provided is a manufacturing method of sutures used for MACO models of mice. The sutures are prepared by selection, preparation of material, coating of head ends and marking after coating. The suture material is a filament which is drawn with the diameter ranging from 0.065 to 0. 1 mm at the temperature of 300 DEG C and made by polytetrafluoroethylene resin. At the temperature of 350 DEG C, the surface of the above filament is coated by titanium dioxide in order to obtain the material of the sutures. Coating material, for coating the head ends, is formed by mixing 15% of silicone emulsion, 34% of polyvinylpyrrolidone, 23% of polyvinyl alcohol, 16% of polyethylene glycol, and 12% of polytetrafluoroethylene under ultrasonic concussion based on weight. The manufacturing method of the sutures used for MACO models of mice has following beneficial effects: the sutures are consistent in size and have appropriate hardness and elasticity and blunt and smooth head ends so that the sutures can smoothly enter middle cerebral arteries of mice; inserted sutures can be bent in accordance with the bending blood vessels in order to reduce mechanical damage to blood vessels; obstruction is performed more completely and reliably; the scope of cerebral ischemia is stable; cerebral infarct volume is stable; operation is carried out easily; high success rate of models is achieved; and death rate of mice is low.

Description

technical field [0001] The invention belongs to the field of life sciences, and in particular relates to a method for making a thread plug for a mouse MCAO model. Background technique [0002] Stroke is a general term for acute cerebrovascular diseases, clinically manifested as hemiplegia, aphasia and other symptoms, and has the characteristics of high fatality rate, high disability rate and high recurrence rate. The latest research shows that stroke has seriously threatened people's life and quality of life, among which ischemic stroke ranks first among patients, accounting for about 80% of all stroke patients. [0003] The animal model of ischemic stroke is the basis for the study of cerebral ischemic injury and treatment. The middle cerebral artery is a predilection site for ischemic stroke. Therefore, the middle cerebral artery occlusion (MCAO) animal model is often used as the research basis. The embolization middle cerebral artery cerebral ischemia model is the prefer...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61D1/00A61L31/04A61L31/08A61L31/10A61L31/16A61L33/10
CPCA61D1/00A61L31/048A61L31/10A61L31/16A61L33/0041A61L33/068A61L2300/42C08L71/00
Inventor 田丰丰赵蕾张颖裕赖晓艺申蒙蒙田真真吴剑男
Owner THE FIRST AFFILIATED HOSPITAL OF HENAN UNIV OF SCI & TECH
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