Preparation method of porcine circovirus type II gene engineering subunit oral vaccine

Abstract

The invention relates to a preparation method of a porcine circovirus type II gene engineering subunit oral vaccine. The method comprises the following steps: 1, sequence synthesis; 2, construction of a recombinant vector in E. coli; 3, construction of a recombinant vector in Lactococcus lactis MG1363; and 4, protein expression, recombinant bacteria preservation experiment, and vaccine preparation. The invention has the following beneficial effects: no special treatment for the antigen avoids antigen purification and reduces antigen loss; optimization of the gene sequence improves the antigen expression; optimization of expression conditions solves the problems of toxicity to animals caused by addition of inducing agent for other prokaryotic expression and high costs, and avoids the increase of the chance of contamination and increase of the workload in the expression process. The method provided by the invention has low cost and significant effect; the invention employs oral vaccine to avoid the problems of stress and absorption caused by routine vaccination.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a preparation method of porcine circovirus type 2 genetic engineering subunit oral vaccine. Background technique [0002] Porcine circovirus type 2 (PCV2) can not only cause multisystemic wasting syndrome (PMWS) in weaned piglets, but also be associated with porcine dermatitis and nephrotic syndrome (PDNS), porcine respiratory disease syndrome (PRDC), and porcine congenital tremor. , sow reproductive disorders, pig hyperplasia and necrotizing pneumonia and other diseases are closely related. Since the discovery of the disease that can cause pigs in the 1890s, the PCV2 virus has spread rapidly in countries around the world. At present, the infection rate of PCV2 in pig herds is very high, which causes the growth of pigs to be slow and the feed remuneration to decrease. At the same time, it infringes on the immune system of pigs, leading to the decline of immune function of...

AI Technical Summary

Problems solved by technology

However, this method needs to purify the protein, and then add reagents such as inactivators and preservatives to emulsify the vaccine and carry out injection immunization. The process of preparing the vaccine is cumbersome and the process is complicated. As a routine injection vaccine, it is easy to cause stress and vaccine absorption problems.

Images