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Method for curing soil mercury with bacteria under anaerobic condition

A technology based on anaerobic conditions and bacteria, applied in the restoration of contaminated soil, etc., to achieve the effect of low treatment cost, simple process and large treatment range

Inactive Publication Date: 2015-09-09
XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, we still need to further study the capture of elemental mercury by amorphous nano-selenium under anaerobic conditions (deep soil). So far, no application of amorphous nano-selenium under anaerobic conditions in soil mercury pollution remediation has been seen.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Strain enrichment culture:

[0026] Bacteria of the genus Taueria ( Thauear selenatis ), transfer the bacteria to ATCC 2483 according to the volume ratio of 3% inoculum Thauear selenatis A medium, the medium components are NaCl 1.2g, KCl 0.3g, NH 4 Cl 0.3g, KH 2 PO 4 0.2g, Na 2 SO 4 0.3g, MgCO 3 ·H 2 O 0.4g, CaCl 2 2H 2 O 0.15g, NaHCO 3 0.6g, yeast extract powder 4g, NaAC 10mmol / L, NaNO 3 10mmol / L, 10ml of ATCC vitamin solution (purchased from the American Type Culture Collection Center), 1 liter of deionized water, cultured in an anaerobic workstation at a temperature of 20°C for 2 days for enrichment;

[0027] Preparation of bacterial metabolites:

[0028] Add the enriched Taueria bacterium solution into ATCC 2483 containing sodium selenate with an initial concentration of 1 mmol / L and a pH value of 4 at an inoculum volume of 3%. Thauear selenatis B medium, the composition of the medium is NaCl 1.2g, KCl 0.3g, NH 4 Cl 0.3g, KH 2 PO 4 0.2g, Na ...

Embodiment 2

[0032] Strain enrichment culture:

[0033] Bacteria of the genus Taueria ( Thauear selenatis ), the bacteria were transferred to ATCC 2483 according to the volume ratio of 6% inoculum Thauear selenatis A medium, the medium components are NaCl 1.2g, KCl 0.3g, NH 4 Cl 0.3g, KH 2 PO 4 0.2g, Na 2 SO 4 0.3g, MgCO 3 ·H 2 O 0.4g, CaCl 2 2H 2 O 0.15g, NaHCO 3 0.6g, yeast extract powder 4g, NaAC 10mmol / L, NaNO 3 10mmol / L, 10ml of ATCC vitamin solution (purchased from the American Type Culture Collection Center), 1 liter of deionized water, cultured in an anaerobic workstation at a temperature of 30°C for 5 days for enrichment;

[0034] Preparation of bacterial metabolites:

[0035] The enriched Taueria bacterium solution was added to ATCC 2483 containing sodium selenate with an initial concentration of 10 mmol / L and a pH value of 7 according to the volume ratio of 6% inoculum. Thauear selenatis B medium, the composition of the medium is NaCl 1.2g, KCl 0.3g, NH 4 Cl...

Embodiment 3

[0039] Strain enrichment culture:

[0040] Taueria Thauear selenatis , transfer the bacteria to ATCC 2483 according to the inoculum of 5% by volume Thauear selenatis A medium, the medium components are NaCl 1.2g, KCl 0.3g, NH 4 Cl 0.3g, KH 2 PO 4 0.2g, Na 2 SO 4 0.3g, MgCO 3 ·H 2 O 0.4g, CaCl 2 2H 2 O 0.15g, NaHCO 3 0.6g, yeast extract powder 4g, NaAC 10mmol / L, NaNO 3 10mmol / L, 10ml of ATCC vitamin solution (purchased from the American Type Culture Collection Center), 1 liter of deionized water, and cultured in an anaerobic workstation at a temperature of 25°C for 4 days for enrichment;

[0041] Preparation of bacterial metabolites:

[0042] The enriched Taueria bacterium solution was added to ATCC 2483 with an initial concentration of sodium selenate of 5 mmol / L and a pH value of 11 at an inoculum volume of 5% by volume. Thauear selenatis B medium, the composition of the medium is NaCl 1.2g, KCl 0.3g, NH 4 Cl 0.3g, KH 2 PO 4 0.2g, Na 2 SO 4 0.3g, Mg...

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Abstract

The invention discloses a method for curing soil mercury with bacteria under the anaerobic condition. The method includes that Thauear selenatis, ATCC 55363 which can be used for dissimilatory reduction of sodium selenite is selected and is from ATCC (American type culture collection), the Thauear selenatis is transferred to a culture medium containing sodium selenite for culture so as to obtain a bacterial dissimilatory reduction metabolite, then mixed liquor containing the metabolite is added with sodium dodecyl sulfate, and finally the treated metabolite is added into elemental mercury contaminated soil for culture in an anaerobic workstation for several days prior to ending experiment. Under the condition of presence of sodium selenite, the anaerobic dissimilatory reduction metabolite is a good curing agent of mercury, the elemental mercury in soil can be cured into residual-form mercury so as to fix the soil mercury and repair soil mercury contamination, and the method is particularly applicable to repair of deep contaminated soil. The method is simple in process, convenient to operate, low in treatment cost, large in treatment range and free of secondary contamination.

Description

technical field [0001] The invention relates to a method for bacterial solidification of soil mercury under anaerobic conditions, and belongs to the technical field of environmental protection soil purification treatment. Background technique [0002] Mercury is the only liquid heavy metal at room temperature and is a global pollutant. Mercury is highly toxic and not bioavailable. Mercury vapor (elemental mercury) mainly causes damage to the lungs, central system, kidneys, etc., and can cause death in severe cases. Inorganic mercury (divalent mercury ions) mainly causes damage to the digestive tract, immune system and kidneys. Organic mercury (mainly methylmercury) is easily absorbed by the digestive tract, mainly causing neurotoxicity, brain and nervous system damage, and also has hereditary damage, so it is more toxic to pregnant women and fetuses. [0003] The large-scale use of mercury in industry (mining metallurgy; chlor-alkali industry; production of fluorescent la...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10
Inventor 潘响亮王潇男宋文娟
Owner XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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