Method and system for extracting and separating novel Schaftoside in Desmodium styracifolium

A technology for new schistoside and quincea quinquefolium, applied in the field of phytochemistry, can solve the problem of no new schistoside and other problems, and achieve the effects of high product yield, short production time and high purity

Active Publication Date: 2015-09-23
WUHAN OPTICS VALLEY HUMANWELL BIO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is currently no new xiafotaside reference substance for sale on the market, and the e

Method used

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  • Method and system for extracting and separating novel Schaftoside in Desmodium styracifolium
  • Method and system for extracting and separating novel Schaftoside in Desmodium styracifolium
  • Method and system for extracting and separating novel Schaftoside in Desmodium styracifolium

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Experimental program
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Embodiment 1

[0031] Figure 5 A general scheme of the method of the invention is shown.

[0032] 1. Raw materials: select 45000-50000 parts by weight of the leguminous plant Desmodium glabrata, cut into 5-10cm sections, wash off the sediment with drinking water, drain and feed.

[0033] 2. Ethanol extraction: reflux extraction with 80% ethanol twice, the first time 12 times the amount for 2 hours, the second time 10 times the amount for 1.5 hours; combine the two alcohol extracts, and recover the ethanol until it has no alcohol smell.

[0034] 3. Concentration under reduced pressure: add water to dilute the extract concentrate to 5 times the volume of the medicinal material; filter it with a 200-mesh filter cloth; get the column liquid; pack the net product resin that has been treated with 95% ethanol in advance, and replace it with purified water; The upper column liquid is pumped into the column for adsorption, and the flow rate is controlled to be 0.5-1 times BV / h. After the adsorption...

Embodiment 2

[0046] 1. Carry out the steps 1-3 of embodiment 1, obtain the herba styrofoam crude drug. 2. Tertiary chromatographic separation

[0047] (1) 300-400 parts by weight of Guangjin crude drug is separated by AB-8 macroporous resin chromatography, that is, chromatographic separation I, ethanol-water mixed solvent 25:75, 35:65, 45:55, 65:35 gradient washing Take off, wash each gradient until there is no color, combine the fractions to get 25%, 35%, 45%, and 65% in four parts.

[0048] (2) Use ODS reverse-phase medium pressure chromatography to separate the 35% part obtained by chromatographic separation I, that is, chromatographic separation II, use ethanol-water, the condition is 8% isocratic for 30 minutes, and 8-30% gradient is eluted for 200 minutes, according to the chromatographic The peak sequence is shown in the figure, and collected in fractions, one portion is collected for every 100-150 volume fractions, a total of 40-50 fractions are collected, and the same fractions a...

Embodiment 3

[0051] 1. Carry out the steps 1-3 of embodiment 1, obtain the herba styrofoam crude drug. 2. Tertiary chromatographic separation

[0052] (1) 300-400 parts by weight of Guangjin crude drug is separated by AB-8 macroporous resin chromatography, that is, chromatographic separation I, ethanol-water mixed solvent 20:80,30:70,40:60,60:40 gradient washing Take off, wash each gradient until there is no color, combine the fractions to get 20%, 30%, 40%, and 60% in four parts.

[0053] (2) Use ODS reverse-phase medium-pressure chromatography to separate 20% of the chromatographic separation I, that is, chromatographic separation II, use ethanol-water, the condition is 12% isocratic for 30 minutes, 12-30% gradient for 200 minutes to elute, according to the chromatographic The peak sequence is shown in the figure, and collected in fractions, one portion is collected for every 200-400 volume fractions, a total of 30-40 fractions are collected, and the same fractions are combined after hi...

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Abstract

The invention provides a method for extracting and separating novel Schaftoside. The method includes the steps of subjecting Desmodium styracifolium to reflux extraction via ethyl alcohol to obtain Desmodium styracifolium extract; subjecting the extract to multistage chromatographic separation via alcohol systems, to be specific, subjecting the extract to first chromatographic separation via the first alcohol system to obtain a first flow, subjecting the first flow to second chromatographic separation via the second alcohol system to obtain a second flow, and subjecting the second flow to third chromatographic separation via the third alcohol system to so as to obtain the novel Schaftoside. The process of separating and extracting the novel Schaftoside from Desmodium styracifolium is simple, production time is short, the yield is high, the purity is high, and the novel Schaftoside is up to above 95% in purity.

Description

technical field [0001] The invention belongs to the technical field of phytochemistry. Specifically, the invention relates to a method and a system for extracting and isolating novel schaftosides from Desmodium sativa. Background technique [0002] Desmodium styracifolium (Osbeck) Merr. is a plant of the leguminous genus Desmodium styracifolium (Osbeck) Merr. Its medicinal part is the dry aerial part, and its main chemical components are flavonoids, saponins, polysaccharides, alkaloids and other compounds. It has the effects of clearing away heat and dehumidification, diuresis and treating stranguria. It is used for hot shower, sand shower, stone shower, astringent and painful urination, edema and oliguria, jaundice, red urine, and urinary calculi. Neo-schaftoside (Neo-schaftoside), also known as 6-C-glucose-8-C-β-arabinose acetin, is light yellow powder; molecular formula: C 26 h 28 o 14 ; Molecular weight: 564, its structural formula is as figure 1 shown. [0003] Ne...

Claims

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Application Information

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IPC IPC(8): C07H17/07C07H1/08
CPCC07D407/14
Inventor 王学海许勇李莉娥杨仲文杨婷余通冯芸尹海龙黄璐杨成兵黄天赐曹儒宾谢长
Owner WUHAN OPTICS VALLEY HUMANWELL BIO PHARMA
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