Continuous detector for multi-wavelength excited fluorescence labeling immune test strips

Abstract

The invention provides a continuous detector for multi-wavelength excited fluorescence labeling immune test strips and belongs to the technical field of fluorescence labeling immune sample detection. The continuous detector consists of a mechanical transmission system, an optical system and an image acquisition system. Fluorescence labeling immune test strips in a test strip placing groove (17) fall into a test strip chute (3) and are pushed to a light source turntable (18) by a rotating chute disk (1) to be detected. An LED excitation light source (12), a slit (13) and a light filter (14) are mounted at the rectangular hole in the light source turntable (18), and fluorescent light is subjected to stray light filtration, focused by a focusing lens (15), then received by a CCD linear array sensor (16) and finally treated by the image acquisition system to realize detection of the immune test strips. The continuous detector has the advantages that continuous on-line detection and different-wavelength fluorescence test strip detection are achieved, light intensity control is realized to lower the test strip preparation demands, noise is eliminated effectively, the wave crests of the detected fluorescent light are accurately read, and the detection precision is improved.

Description

technical field [0001] The invention belongs to the technical field of fluorescently labeled immune sample detection, and is an instrument for continuously detecting laser-induced fluorescently labeled immune test strips. The device can perform continuous and rapid detection and result judgment on fluorescently labeled immune test strips with four wavelengths and The wavelength area is accurately calculated to realize multi-target detection in the fields of medicine, environment, chemical industry, virus and so on. Background technique [0002] Immunofluorescence detection uses fluorescent substances (also known as fluorochromes or fluoresceins) as markers to label antibodies (or antigens), react with corresponding antigens (or antibodies), and detect the presence or absence of fluorescence according to the presence or absence of fluorescence. . The fluorescence detector is based on the quantitative detection of fluorescence energy, and uses the machine instead of human vis...

AI Technical Summary

Problems solved by technology

Due to the relatively poor thermal stability of the laser light intensity, it is easy to cause drift errors. The cost of a laser with stable performance is high, and the manufacturing process is relatively complicated, so it is not easy to realize

Using photodiode and photomultiplier tube to detect the fluorescence emitted by the object after optical condensing will be interfered by other background light, and the degree of influence is relatively large

When the photomultiplier tube is used as a fluorescence detection component, its photon conversion efficiency is only 5-10% when the fluorescence wavelength of the immunoassay strip is about 600nm, so it is not suitable for detecting low-concentration samples

Moreover, the existing fluorescent test paper detection instrument can only detect one test paper strip at a time, and the detection wavelength is fixed, so the use of the instrument is limited.

The time required for different detected objects is different, and it is difficult for the operator to grasp the best detection time, which affects the detection accuracy and wastes a lot of time and financial resources

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