Tissue culture and rapid propagation method of luculia yunnanensis

A technique for tissue culture and rapid propagation of Lilac cockscomb Dianensis, which is applied to the field of tissue culture and rapid propagation of lilac cockscomb and Dianna cockscomb, can solve problems that have not been seen before, and achieve the advantages of less materials, high reproduction coefficient, and easy material acquisition. Effect

Active Publication Date: 2015-10-07
THE RES INST OF RESOURCES INSECTS RIRI OF THE CHINESE ACADEMY OF FORESTRY
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there is an urgent need to study and summarize the breeding techno

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Tissue culture and rapid propagation method of luculia yunnanensis
  • Tissue culture and rapid propagation method of luculia yunnanensis
  • Tissue culture and rapid propagation method of luculia yunnanensis

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0023] Example 1

[0024] A. After washing the wild cockscomb lilac capsules with water, move them to the ultra-clean workbench, soak them in 75% alcohol for 30s under aseptic conditions, rinse them with sterile water 3 times quickly, and then use the mass concentration Soak the 0.1% mercury solution for 20 minutes, rinse with sterile water 5 times, then absorb the surface water with sterile filter paper, cut the capsule with a scalpel to take the seeds, and then insert the seeds into the following primary culture medium:

[0025] MS

[0026] Sucrose 30g / L,

[0027] When the temperature is 25℃, the light time is 12h / d, the light intensity is 40μmol / m 2 Cultivation under the conditions of s; under this condition, the seeds will begin to germinate in about 10 days, and the average height of the seedlings will be 2.25 cm by 50 days;

[0028] B. Cut the seedlings cultivated in step A, cut into stem segments with axillary buds and inoculate them in the following propagation medium:

[0029] ...

Example Embodiment

[0037] Example 2

[0038] A. After washing the wild cockscomb lilac capsules with water, move them to an ultra-clean workbench, soak them in 75% alcohol for 30s under aseptic conditions, and quickly rinse them with sterile water for 5 times, and then use the mass concentration to Soak the 1.0% mercury solution for 10 minutes, rinse with sterile water 8 times, then absorb the surface moisture with sterile filter paper, cut the capsule with a scalpel to take the seeds, and then insert the seeds into the following primary culture medium:

[0039] MS

[0040] Sucrose 25g / L,

[0041] When the temperature is 20℃, the light time is 8h / d, and the light intensity is 35μmol / m 2 Cultivation under the conditions of s; under this condition, the seeds will begin to germinate after 10 days, and the seedlings will grow to 2.96 cm in 60 days;

[0042] B. Cut the seedlings cultivated in step A, cut into stem segments with axillary buds and inoculate them in the following propagation medium:

[0043]

[0...

Example Embodiment

[0051] Example 3

[0052] A. After washing the wild cockscomb lilac capsules with water, move them to an ultra-clean workbench, soak them in 75% alcohol for 30s under aseptic conditions, and quickly rinse with sterile water for 4 times, and then use the mass concentration to Soak the 0.5% mercury solution for 15 minutes, rinse with sterile water 6 times, then absorb the surface moisture with sterile filter paper, cut the capsule with a scalpel to take the seeds, and then put the seeds into the following primary culture medium:

[0053] MS

[0054] Sucrose 35g / L,

[0055] When the temperature is 30℃, the light time is 10h / d, the light intensity is 45μmol / m 2 Cultivate under the condition of s until the height of the seedlings is 2~3cm; cultivate under this condition until the seeds begin to germinate in about 10 days, and the seedlings can grow 2.61cm in height by 55 days;

[0056] B. Cut the seedlings cultivated in step A, cut into stem segments with axillary buds and inoculate them in...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a tissue culture and rapid propagation method of luculia yunnanensis. The method comprises the following steps: inoculating seeds into a primary culture medium for culturing an aseptic seedling, then cutting the aseptic seedling into stems with axillary buds, inoculating the stems into a multiplying culture medium for culturing a cluster bud, cutting off the cluster bud, inoculating the cluster bud into a rooting culture medium for culturing a rooted seedling, and exercising the seedling to obtain a transplanting seedling. By adopting the method, not only is the material easy and convenient to get in the field, but also a great quantity of excellent seedlings can be propagated by utilizing fewer materials in a short period; moreover, the limitation of seasons and regions can be avoided, the repeatability is good, the survival rate of transplanted seedlings reaches 90 percent or more, and the demand of the market on the luculia yunnanensis can be completely satisfied.

Description

technical field [0001] The invention relates to a method for tissue culture and rapid propagation of plants, in particular to a method for tissue culture and rapid propagation of Lilac cockscomb, belonging to the technical field of plant tissue culture. Background technique [0002] Cockscomb Yunnan lilac (Luculia yunnanensis) belongs to Rubiaceae (Rubiaceae) Yunnan lilac genus (Luculia), and it is obviously different from other hairless species in this genus by densely pilose at the tip of branchlets, calyx tube and fruit. In addition, Lilac cockscomb is the only plant endemic to my country in the genus Syringa, which is mainly distributed in Nujiang, Lincang and Simao in Yunnan. The leaves of the plant are emerald green, the plant shape is beautiful, and the tops of the branches are clustered with corymbose cymes. The smell is fragrant and the flowering period is long. It has high ornamental value and broad application prospects. The distribution area of ​​Lilac cockscomb...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H4/00
Inventor 马宏刘秀贤李正红万友名
Owner THE RES INST OF RESOURCES INSECTS RIRI OF THE CHINESE ACADEMY OF FORESTRY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap