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Tissue culture and rapid propagation method of luculia yunnanensis

A technique for tissue culture and rapid propagation of Lilac cockscomb Dianensis, which is applied to the field of tissue culture and rapid propagation of lilac cockscomb and Dianna cockscomb, can solve problems that have not been seen before, and achieve the advantages of less materials, high reproduction coefficient, and easy material acquisition. Effect

Active Publication Date: 2015-10-07
THE RES INST OF RESOURCES INSECTS RIRI OF THE CHINESE ACADEMY OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there is an urgent need to study and summarize the breeding technology of Lilac cockscomb, but there are no relevant reports at home and abroad.

Method used

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  • Tissue culture and rapid propagation method of luculia yunnanensis
  • Tissue culture and rapid propagation method of luculia yunnanensis
  • Tissue culture and rapid propagation method of luculia yunnanensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A. After washing the wild Clove cockscomb capsules with water, move them to the ultra-clean workbench, soak them in alcohol with a volume concentration of 75% for 30s under aseptic conditions, rinse them with sterile water for 3 times quickly, and then wash them with a mass concentration of Soak in 0.1% mercuric chloride solution for 20 minutes, rinse 5 times with sterile water, then blot the surface moisture with sterile filter paper, cut open the capsule with a scalpel to take the seeds, and then insert the seeds into the following primary medium:

[0025] MS

[0026] Sucrose 30g / L,

[0027] When the temperature is 25°C, the light time is 12h / d, and the light intensity is 40μmol / m 2 Cultivate under the condition of s; cultivate under this condition until about 10 days before the seeds start to germinate, and the average height of the seedlings is 2.25cm after 50 days;

[0028] B, the seedling that step A is cultivated is cut off, is cut into the stem section of band...

Embodiment 2

[0038] A. After washing the wild Cockscomb Diancilia capsules with water, move them to the ultra-clean workbench, soak them in 75% alcohol for 30s under aseptic conditions, rinse them with sterile water for 5 times, and then wash them with a mass concentration of Soak in 1.0% mercuric chloride solution for 10 minutes, rinse 8 times with sterile water, then blot the surface moisture with sterile filter paper, cut the capsules with a scalpel to take the seeds, and then insert the seeds into the following primary culture medium:

[0039] MS

[0040] Sucrose 25g / L,

[0041] When the temperature is 20°C, the light time is 8h / d, and the light intensity is 35μmol / m 2 Cultivate under the condition of s; cultivate under this condition until the seeds start to germinate in about 10 days, and the seedlings grow up to 2.96cm in 60 days;

[0042] B, the seedling that step A is cultivated is cut off, is cut into the stem section of band axillary bud and is inoculated in the following prop...

Embodiment 3

[0052] A. After washing the wild Cockscomb Diancilia capsules with water, move them to the ultra-clean workbench, soak them in alcohol with a volume concentration of 75% for 30s under aseptic conditions, rinse them with sterile water for 4 times, and then wash them with a mass concentration of Soak in 0.5% mercuric chloride solution for 15 minutes, rinse 6 times with sterile water, then blot the surface moisture with sterile filter paper, cut open the capsule with a scalpel to take the seeds, and then insert the seeds into the following primary medium:

[0053] MS

[0054] Sucrose 35g / L,

[0055] When the temperature is 30°C, the light time is 10h / d, and the light intensity is 45μmol / m 2Cultivate under the condition of s until the height of the seedlings is 2 to 3 cm; under this condition, the seeds start to germinate after about 10 days, and the seedlings can grow to a height of 2.61 cm after 55 days;

[0056] B, the seedling that step A is cultivated is cut off, is cut int...

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Abstract

The invention discloses a tissue culture and rapid propagation method of luculia yunnanensis. The method comprises the following steps: inoculating seeds into a primary culture medium for culturing an aseptic seedling, then cutting the aseptic seedling into stems with axillary buds, inoculating the stems into a multiplying culture medium for culturing a cluster bud, cutting off the cluster bud, inoculating the cluster bud into a rooting culture medium for culturing a rooted seedling, and exercising the seedling to obtain a transplanting seedling. By adopting the method, not only is the material easy and convenient to get in the field, but also a great quantity of excellent seedlings can be propagated by utilizing fewer materials in a short period; moreover, the limitation of seasons and regions can be avoided, the repeatability is good, the survival rate of transplanted seedlings reaches 90 percent or more, and the demand of the market on the luculia yunnanensis can be completely satisfied.

Description

technical field [0001] The invention relates to a method for tissue culture and rapid propagation of plants, in particular to a method for tissue culture and rapid propagation of Lilac cockscomb, belonging to the technical field of plant tissue culture. Background technique [0002] Cockscomb Yunnan lilac (Luculia yunnanensis) belongs to Rubiaceae (Rubiaceae) Yunnan lilac genus (Luculia), and it is obviously different from other hairless species in this genus by densely pilose at the tip of branchlets, calyx tube and fruit. In addition, Lilac cockscomb is the only plant endemic to my country in the genus Syringa, which is mainly distributed in Nujiang, Lincang and Simao in Yunnan. The leaves of the plant are emerald green, the plant shape is beautiful, and the tops of the branches are clustered with corymbose cymes. The smell is fragrant and the flowering period is long. It has high ornamental value and broad application prospects. The distribution area of ​​Lilac cockscomb...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 马宏刘秀贤李正红万友名
Owner THE RES INST OF RESOURCES INSECTS RIRI OF THE CHINESE ACADEMY OF FORESTRY
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