Method for effectively extracting anthocyanidin and anthocyanin

An anthocyanin and anthocyanin technology, applied in chemical instruments and methods, organic dyes, organic chemistry, etc., to achieve the effects of low production equipment requirements, strong operability, and low energy consumption

Active Publication Date: 2015-10-07
ZHEJIANG HUISONG PHARMA
View PDF16 Cites 20 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a medicine, the purity requirement is generally high, and the simple extraction and purification process is difficult to make the anthocyanin content reach the international general standard (anthocyanin ≥ 25.0% by ultraviolet measurement, anthocyanin ≥ 36.0% by liquid phase measurement), which is restricting my country's bilberry The bottleneck of the extract industry, this situation needs to be improved urgently

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Take out 25 kg of lingonberry fresh fruit raw materials from the cold storage, feed them into the extraction tank within 30 minutes, add 75 kg of 90% ethanol solution at 0°C for extraction, stir at a speed of 3000 rpm, extract for the first time for 2 hours, and collect by filtration For the extract, 50 kg of 70% ethanol solution was added for the second and third times for extraction for 1 hour, the extract was collected by filtration, and the three extracts were combined. Concentrate the combined extracts under reduced pressure until there is no alcohol smell, the temperature of the concentrated under reduced pressure is 50° C., the degree of vacuum is 0.08 MPa, and the ethanol is recovered to obtain the concentrated solution. The concentrated solution is centrifuged with a disc separator, the centrifugation interval is 6 minutes, the residue is discarded, the slag discharge time is 6 seconds, and the supernatant is retained. The supernatant was refined with a weakly ...

Embodiment 2

[0026] Take out 25 kg of blackcurrant fresh fruit raw materials from the cold storage, feed them into the extraction tank within 30 minutes, add 100 kg of 70% ethanol solution at 10°C for extraction, stir at a speed of 1600 rpm, extract for the first time for 1 hour, and collect by filtration For the extract, 50 kg of 70% ethanol solution was added for the second and third extractions for 2 hours, the extract was collected by filtration, and the three extracts were combined. The combined extracts were concentrated under reduced pressure until there was no alcohol smell, the temperature of the concentrated under reduced pressure was 45° C., the vacuum degree was 0.085 MPa, and the ethanol was recovered to obtain a concentrated solution. The concentrate is centrifuged with a disk separator, the separation interval is 8 minutes, the residue is discarded, the residue discharge time is 7 seconds, and the supernatant is retained. The supernatant was refined with a weakly polar CAD-4...

Embodiment 3

[0028]Take out 25 kg of blueberry fresh fruit raw materials from the cold storage, feed them into the extraction tank within 30 minutes, add 50 kg of 80% ethanol solution to extract at a temperature of 20 ° C, stir at a speed of 2300 rpm, extract for the first time for 2 hours, and collect and extract by filtration 100 kg of 90% ethanol solution was added for the second and third times to extract for 2 hours, the extracts were collected by filtration, and the three extracts were combined. The combined extracts were concentrated under reduced pressure until there was no alcohol smell, the temperature of the concentrated under reduced pressure was 40° C., the vacuum degree was 0.09 MPa, and the ethanol was recovered to obtain a concentrated solution. The concentrate is centrifuged with a disk separator, the separation interval is 10 minutes, the residue is discarded, the residue discharge time is 10 seconds, and the supernatant is retained. Purify the supernatant with a weakly p...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for effectively extracting anthocyanidin and anthocyanin, and the method comprises the following steps: (1) extracting fresh anthocyanidin and anthocyanin raw materials, performing rapid stirring and extracting with 70-90% ethanol solution for 2-3 times, mixing and filtering obtained an extraction solution, performing vacuum concentration of the extraction solution to alcohol-free state, and obtaining the a concentrate; (2) centrifuging the concentrate obtained from the step (1), preserving a supernatant, refining the supernatant with CAD-40 macroporous adsorption resin, performing cleaning with purified water, performing washing out with 65-75% ethanol and collecting the eluate; (3) performing vacuum concentration of the eluate to the non-alcohol state so as to obtain a concentrate, and carrying out spray drying of the concentrate and obtaining the extract product containing anthocyanidin and anthocyanin. According to the invention, the method for effectively extracting anthocyanidin and anthocyanin is provided, and the method has the advantages of simple production equipment, short production period and low cost, and substantially increased purities of the effective components in the extract product.

Description

technical field [0001] The invention relates to a method for effectively extracting anthocyanins and anthocyanins. Background technique [0002] Anthocyanidin, also known as anthocyanidin, is a kind of water-soluble natural pigment widely present in plants in nature, belonging to flavonoid compounds. It is also the main coloring substance in plant petals, and most of the colorful colors of fruits, vegetables, and flowers are related to it. Anthocyanin is a pure natural anti-aging nutritional supplement. Studies have shown that it is the most effective antioxidant found by humans today. Its antioxidant performance is fifty times higher than vitamin E and twenty times higher than vitamin C. It is 100% bioavailable to the human body and can be detected in the blood twenty minutes after ingestion. [0003] Anthocyanins are a class of compounds formed by the combination of anthocyanins and sugars through glycosidic bonds. They are widely present in the cell fluids of flowers, f...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/065C07D311/62C07H1/08C09B61/00
Inventor 郑猛马全民聂复礼杨浩王青青
Owner ZHEJIANG HUISONG PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap