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Determination method for HPLC (High Performance Liquid Chromatography) fingerprint of rhizoma atractylodis and cortex phellodendri pain removing preparation
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A determination method and fingerprint technology, which is applied in the field of determination of HPLC (high performance liquid chromatography) fingerprint of Cangbai pain preparation, which can solve the problems of poor patient compliance, bad smell, and large doses taken by patients
Inactive Publication Date: 2015-10-07
甘肃德申科技发展有限公司
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However, the combined use of allopurinol has high toxicity and side effects, and it is prone to rash, diarrhea, nausea, vomiting, stomach pain, numbness and tingling of hands and feet, allergic vasculitis and other adverse reactions. Drugs have disadvantages such as poor stability, bad smell, and large doses taken by patients, resulting in poor patient compliance, so the development of new preparations is imperative
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[0030] Experimental example: Determination of HPLC fingerprint of Cangbai Qutong preparation
[0034] Berberinehydrochloride reference substance (batch number 110713-201212), phellodine hydrochloride reference substance (batch number 111895-201202), gentiopicroside (batch number 110770-201314), paeoniflorin (batch number 110736-201438), tetrandrine (batch number 110711-200708) were purchased from China National Institutes for Food and Drug Control, Cangbai pain relief preparation (capsule) (produced by the preparation room of Lanzhou Pulmonary Hospital, batch numbers are 20130828, 20130925, 20131017, 20131126, 20131218, 20140121, 20140226, 20140312 , 20140415, 20140513). Acetonitrile is chromatographical...
Embodiment 1
[0107] Cangbai Pain Capsules (1500g of Cortex Phellodendri, 1500g of Cangzhu (bran fried), 1000g of Sichuan Achyranthes bidentata, 1200g of Fangji, 1200g of Radix Paeoniae Rubra, 1200g of Moutan Cortex, 500g of Asarum, 1500g of SophoraSophora, 1200g of Gentiana chinensis (Mahua Gentiana), 1200g of pine knots 1500g, 1000g of Chinese herb, 1500g of day lily (raw), 200g of licorice and 1000g of comfrey, crushed, sieved, mixed, and prepared according to the conventional capsule preparation method of pharmacy).
[0109] Take 10 batches of the above-mentioned capsule samples, take the samples of this product under the difference in loading amount, mix them evenly, grind them finely, take about 2g, accurately weigh them, put them in a stoppered Erlenmeyer flask, and accurately add acetonitrile-0.1wt% phosphoric acid (36 :64, V / V) 25mL, sealed, weighed, ultrasonic treatment (power 250 W, frequency 50kHz) for 30 minutes, took out, allowed t...
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Abstract
The invention discloses a determination method for HPLC (High Performance Liquid Chromatography) fingerprint of a rhizoma atractylodis and cortex phellodendri pain removing preparation, which comprises the following steps: (1) extracting acetonitrile-phosphoric acid to obtain a solution for test objects; (2) putting the solution for test objects on a high performance liquid chromatograph to detect and obtain an HPLC fingerprint, wherein the chromatographic conditions are as follows: a chromatographic column is C18 column, and a detection wavelength is 284nm; performing gradient elution by taking acetonitrile-0.1wt percent of phosphoric acid as a mobile phase, wherein volume ratio change of acetonitrile in the mobile phase during the gradient elution over time is as follows: 10 percent to 18 percent during 0 to 15min, 18 percent to 50 percent during 15 to 30min and 50 percent during 30 to 35min. The determination method has good precision, stability, and repeatability on common peaks of drugs of different batches, and the relative retention time and an RSD (Relative Standard Deviation) value of the method as well as the specific value of the uncommon peak total area to the total peak area all meet the requirements of the Specification of Fingerprint Study of Traditional Chinese Medicine Injection (provisional).
Description
technical field [0001] The invention belongs to the field of quality detection of traditional Chinese medicine preparations, and in particular relates to a method for measuring the HPLC (high performance liquid chromatography) fingerprint of Cangbai anti-pain preparation. Background technique [0002] The prescription of Cangbai Tengtong Powder is derived from the classic prescription of Jia Qifeng, a famous old Chinese doctor in Gansu Province. The effect of dredging collaterals and relieving pain. The drug is often used in combination with allopurinol clinically to relieve the symptoms of redness, swelling, heat, and pain during the acute attack of gout, and can further stabilize the blood uric acid level of gout patients so as to control the recurrence of gout. However, the combined use of allopurinol has high toxicity and side effects, and it is prone to rash, diarrhea, nausea, vomiting, stomach pain, numbness and tingling of hands and feet, allergic vasculitis and othe...
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