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Hot water extraction method of dunaliella salina polysaccharide

A technology of hot water extraction and salina polysaccharide, applied in the field of bioengineering, can solve the problems of incomplete process, insufficient extraction, large loss of polysaccharide, etc., and achieve the effects of shortening alcohol precipitation time, saving extraction time, and increasing extraction amount

Inactive Publication Date: 2015-10-14
中山鼎晟生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Using the difference in solubility, according to the nature of the extracted polysaccharides, select an appropriate solvent. Cold extraction or hot extraction are the two main traditional polysaccharide extraction methods. However, the traditional extraction process has imperfect processes, insufficient extraction or polysaccharide extraction. Disadvantages such as large losses

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A hot water extraction method of salina polysaccharide, comprising the following steps:

[0026] (1) Remove impurities and β-carotene: take 10g of Prototheca powder, add 5 times the volume of purified water, and ultrasonically mix for 1 hour at 28°C, filter, take the filtrate, add acetone 10 times the volume of the filtrate, and heat at 28°C Ultrasonic mixing for 1 hour, suction filtration, adding acetone again, mixing under the same conditions, suction filtration, drying to remove β-carotene, and obtaining salina powder without β-carotene;

[0027] (2) Removal of protein: take the salina powder from which β-carotene has been removed in step (1), add 0.5mol / L hydrochloric acid aqueous solution to adjust the pH to 3, and add 1% sodium chloride aqueous solution, let stand for 1 hour, and Centrifuge at 4000r / min for 10min, take the supernatant, transfer the supernatant to a water-bathed Erlenmeyer flask, add 5 times the amount of ethyl acetate to soak in the Erlenmeyer fla...

Embodiment 2

[0030] A hot water extraction method of salina polysaccharide, comprising the following steps:

[0031] (1) Remove impurities and β-carotene: take 20g of Prototheca powder, add 10 times the volume of purified water, and ultrasonically mix for 3 hours at 35°C, filter, take the filtrate, add acetone 20 times the volume of the filtrate, and heat at 35°C Ultrasonic mixing for 3 hours, suction filtration, adding acetone again, mixing under the same conditions, suction filtration, drying to remove β-carotene, and obtaining salina powder with β-carotene removed;

[0032] (2) Removal of protein: take the salt algae powder from which β-carotene has been removed in step (1), add 2mol / L hydrochloric acid aqueous solution to adjust the pH to 3.5, and add 2% sodium chloride aqueous solution, let it stand for 2h, and heat it at 6000r / min centrifuged for 20min, take the supernatant, transfer the supernatant to a water bath Erlenmeyer flask, add 8 times the amount of ethyl acetate to soak in...

Embodiment 3

[0035] A hot water extraction method of salina polysaccharide, comprising the following steps:

[0036] (1) Remove impurities and β-carotene: Take 15g of Prototheca powder, add 8 times the volume of purified water, and ultrasonically mix at 30°C for 2 hours, filter, take the filtrate, add acetone 15 times the volume of the filtrate, and heat at 30°C Ultrasonic mixing for 2 hours, suction filtration, adding acetone again, mixing under the same conditions, suction filtration, drying to remove β-carotene, and obtaining salina powder with β-carotene removed;

[0037] (2) Removal of protein: take the salt algae powder from which β-carotene has been removed in step (1), add 1mol / L sodium hydroxide aqueous solution to adjust the pH to 3.3, and add 2% sodium acetate aqueous solution, let it stand for 1.5h, Centrifuge at 5000r / min for 15min, take the supernatant, transfer the supernatant to a water-bathed Erlenmeyer flask, add 5-8 times the amount of ethyl acetate to soak in the Erlenm...

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PUM

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Abstract

The invention relates to a hot water extraction method of dunaliella salina polysaccharide. The hot water extraction method comprises the following steps: firstly, taking raw algae powder, performing ultrasonic blending, acetone washing and the like on the raw algae powder so as to remove impurities and beta-carotene; adding a PH regulator to regulate PH, adding a saline solution, leaving the solution to stand, performing centrifugation, adding ethyl acetate and distilled water, performing water bath soaking, performing alcohol precipitation, and performing suction filtration so as to obtain course polysaccharide; and finally, measuring polysaccharide content by adopting an anthrone-sulfuric acid method. Through the adoption of the hot water extraction method disclosed by the invention, the alcohol refluxing extraction can be avoided, the loss of polysaccharide in dunaliella salina can be obviously reduced, and the extraction amount of polysaccharide is increased; after the beta-carotene and protein are removed from the dunaliella salina, the ethyl acetate and the distilled water of 150-200ml are added in the dunaliella salina without the beta-carotene or the protein, water bath heating is performed, and the PH is regulated to 7-8, so that the ultrasonic extracting time can be shortened to 60-100min; therefore, when the extraction rate of the polysaccharide is not influenced, the extracting time is greatly shortened; the whole experimental temperature is controlled to be below 75 DEG C, so that the polysaccharide can be prevented from being damaged by excessively high temperature.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a hot water extraction method of salina polysaccharide. Background technique [0002] Salina, belonging to Chlorophyta, Chlorophyta, Volvox, Salinaceae, Salina genus, also known as Dunaliella salina, is a kind of halophilic algae with tiny shape, no cell wall and bare protoplasm. algae. Salina is rich in polysaccharides, trace elements, protein, highly unsaturated fatty acids and β-carotene and other nutrients, and is recognized as one of the main sources of natural β-carotene at home and abroad. At present, the extraction of β-carotene from salina has been industrialized, and the extracted algae residue still contains various vitamins, minerals and polysaccharides. At present, it has been found that salina has various physiological activities such as anti-virus, anti-tumor and immune regulation. Studies have shown that salina polysaccharide has various biological act...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 郭狄
Owner 中山鼎晟生物科技有限公司
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