Biosensor for detecting hydrogen peroxide and polyphenol compounds at high sensitivity, and preparation and application thereof

A polyphenolic compound and biosensor technology, applied in the field of bioanalysis, can solve the problems of complex sensor preparation process and low sensitivity, and achieve the effect of promoting electron transfer, simple process and good repeatability
CN104977337AInactive Publication Date: 2015-10-14NANJING UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANJING UNIV OF SCI & TECH
Publication Date
2015-10-14
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a biosensor for detecting hydrogen peroxide and polyphenol compounds at high sensitivity, and preparation and application thereof. The biosensor is a biomimetic sensor, which is formed from 1-pyrenebutyric acid and hemachrome through the coordination chemical effect of zirconium ions, and is characterized by connecting PEDOT (Polymer Ethylenedioxy Thiophene) with 1-pyrenebutyric acid through phi-phi conjugation, wherein the hemachrome is taken as a main body, PEDOT is taken as a decorative material and 1-pyrenebutyric acid is taken as an intermediate connection agent. PEDOT is a conductive macromolecule capable of greatly promoting electron transfer, so that the detection sensitivity of the sensor is increased; 1-pyrenebutyric acid and PEDOT are connected through phi-phi conjugation, the phi-phi conjugation acting force is very strong, and 1-pyrenebutyric acid can be stably connected onto PEDOT; the hemachrome is connected onto 1-pyrenebutyric acid through coordination formed by zirconium ions and carboxyl, the conjugation effect of zirconium ions is easy to form, and more hemachrome can be combined on the surface of an electrode of same area; and the whole biomimetic sensor is mainly prepared by polymerizing and immersing, and the manner is simple to operate and good in repeatability.
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Description

technical field

[0001] The invention belongs to the technical field of bioanalysis, and in particular relates to a biosensor, its preparation and its application, in particular to a biosensor for highly sensitive detection of hydrogen peroxide and polyphenol compounds, its preparation and its application. Background technique

[0002] Porphyrin and its derivative compounds widely exist in important organelles related to energy transfer in organisms. Iron porphyrin (Hemin) is the active center of hemoglobin and plays an important role in the process of transporting oxygen. Hemoglobin has a similar structure to iron porphyrin, and ferric ion is its active center. Hemoglobin has extremely high catalytic performance for nitrogen, oxygen, nitric oxide, carbon dioxide and peroxide. The ferric ions at the position are provided in the process of gaining and losing electrons. However, due to the lack of a suitable biomimetic environment, its electron transfer efficiency is much low...

Claims

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