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Method for preparing velvet antler protein extracts

A technology of protein extract and velvet antler, which is applied in the field of preparation of velvet antler protein extract, can solve the problems of affecting the efficacy of velvet antler and the low extraction rate of active substances, and achieve the effect of fully utilizing the medicinal value, maintaining extraction efficiency, and high extraction efficiency

Active Publication Date: 2015-10-21
吉林玉参医药科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the research on velvet has a long history, the traditional processing technology is mainly based on repeated boiling, baking and air-drying, the extraction rate of active substances is low, and the destruction of protein and other components significantly affects the efficacy of velvet

Method used

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  • Method for preparing velvet antler protein extracts
  • Method for preparing velvet antler protein extracts
  • Method for preparing velvet antler protein extracts

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preparation example Construction

[0072] The invention discloses a preparation method of velvet antler protein extract, and those skilled in the art can learn from the content of this article and appropriately improve the process parameters to realize it. In particular, it should be pointed out that all similar replacements and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention. The method and application of the present invention have been described through preferred embodiments, and the relevant personnel can obviously make changes or appropriate changes and combinations to the method and application described herein without departing from the content, spirit and scope of the present invention to realize and Apply the technology of the present invention.

[0073] The velvet, reagents or instruments used in the preparation method of the velvet antler protein extract provided by the present invention can be purchased from the market.

Embodiment 1

[0075] The preparation of embodiment 1 deer antler protein extract

[0076] Using velvet antler (hair peach) in a specific growth period as raw material, the velvet protein extract is prepared according to the following process:

[0077] 1) Take the velvet antler (hair peach) in the initial growth stage, and after 6 hours of blood drawing, remove the surface hair, cut into thin slices, freeze in a freeze dryer, and take it out after the weight change is less than 0.1g twice. Put the freeze-dried velvet antler into an appropriate amount of liquid nitrogen, grind it in a mortar, and pass it through a 200-mesh sieve to obtain velvet antler powder.

[0078] 2) Weigh 1 g of velvet antler powder, add 10 mL of Tris-base solution with a concentration of 50 mM, and the pH value is 7.4. After shaking for 30 minutes, homogenize with a homogenizer for 1 min under ice bath.

[0079] 3) Extraction is performed with an ultrasonic cell disruptor, ultrasonicated for 5 seconds with an interva...

Embodiment 2

[0080] The preparation of embodiment 2 deer antler protein extract

[0081] Using velvet antler (two bars) in a specific growth period as raw material, the velvet protein extract is prepared according to the following process:

[0082] 1) Take the velvet antler (two bars) in the initial growth stage, 6 hours after the blood draw, remove the surface hair, cut into thin slices, freeze in a freeze dryer, and take out when the weight change is less than 0.1g twice. Put the freeze-dried velvet antler into an appropriate amount of liquid nitrogen, grind it in a mortar, and pass it through a 200-mesh sieve to obtain velvet antler powder.

[0083] 2) Weigh 1 g of velvet antler powder, add 10 mL of Tris-base solution with a concentration of 50 mM, and the pH value is 7.4. After shaking for 30 minutes, homogenize with a homogenizer for 1 min under ice bath.

[0084] 3) Extraction is performed with an ultrasonic cell disruptor, ultrasonicated for 5 seconds with an interval of 15 second...

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Abstract

The invention relates to the technical field of protein extraction, in particular to a method for preparing velvet antler active protein extracts. The method comprises the steps of conducting blood drawing, freeze-drying and smashing on fresh velvet antler to obtain velvet antler powder; mixing the velvet antler powder with a buffer solution, conducting ultrasonic extraction and centrifugation, collecting supernate, and conducting vacuum freeze drying to obtain the velvet antler protein extracts, wherein the pH value of the buffer solution is 7.0-8.0, and the concentration of the buffer solution is 10-50 mM. By the adoption of the method, the biological activity and extraction efficiency of velvet antler protein are maintained to the maximum degree. The velvet antler protein extracts prepared with the method can promote growth or proliferation of vascular cells, and particularly, the proliferation rate of human umbilical vein endothelial cells (HUVEC) reaches 200%.

Description

technical field [0001] The invention relates to the technical field of protein extraction, in particular to a preparation method of velvet antler protein extract. Background technique [0002] Deer antler is a traditional Chinese medicine with a history of more than 2,000 years. Li Shizhen said in "Compendium of Materia Medica" that velvet antler is "good at nourishing the kidney and strengthening yang, producing essence and blood, nourishing marrow and strengthening bones". The classic medical book "Shen Nong's Materia Medica" also has detailed records of its functions and indications: "It is sweet in taste and warm in nature, and it is mainly used to leak bad blood, cold and heat convulsions, nourishing qi and strengthening will, and the population is not old." Deer antler is warm but not dry, has the function of invigorating and improving the body function, and has a good effect on strengthening the body for patients who are weak and chronically ill. Modern medicinal ch...

Claims

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Application Information

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IPC IPC(8): C07K1/14A61K38/02A61K35/32A61P9/00A61P9/10
Inventor 霍虹霍玉书范一鸣
Owner 吉林玉参医药科技有限公司
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