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Preparation process of bacterial protein from fruit and vegetable lactic acid bacteria fermentation liquid

A technology of fruit and vegetable lactic acid bacteria and preparation technology, which is applied in the field of food processing, and can solve the problems of fermented bacteria such as fruit and vegetable fermentation efficiency, quality and flavor are greatly affected, vitality, acid production, acid resistance, unsatisfactory flavor, and poor bacterial protein activity , to prevent the death of living bacteria and protein denaturation, improve the overall economic benefits, and improve the activity

Active Publication Date: 2018-01-09
辽宁省农业科学院食品与加工研究所 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the fruit and vegetable fermentation process, the quality of the fermentation strains has a great influence on the fermentation efficiency, quality and flavor of the fruits and vegetables. At present, the strains used in the lactic acid fermentation of fruits and vegetables are single, and a few of them are Lactobacillus plantarum, Lactobacillus reinneri, short lactobacillus Bacillus or bifidobacteria are compounded into a starter, but its vitality, acid production, acid resistance, and flavor are not ideal
Moreover, the strains used in conventional lactic acid bacteria fermented preserved fruits and vegetables, sauces and beverage products are not suitable for producing bacterial protein from fermentation broth, and there are technical problems such as poor activity of bacterial protein after fermentation, low yield, and poor flavor.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Take 400 grams of fresh apples, 300 grams of pumpkins, and 300 grams of carrots as raw materials. After washing, slice them into 1 cm thick slices, blanch them at 90°C for 4 minutes, then cool them down to 25°C, put them into a fermentation tank, and add 30 grams of lactic acid bacteria to ferment them. The lactic acid bacteria starter is a lactic acid bacteria starter that is composed of Streptococcus lactis, Lactobacillus delbrueckii, Bifidobacterium, Lactobacillus plantarum, and Lactobacillus acidophilus in a mass ratio of 3:2:1:2:1. Formulated, the total number of lactic acid bacteria in the starter 10 9 -10 11 / ml, sealed fermentation, the fermentation temperature is controlled at 25°C, the fermentation period is 10 days, and the pH value of the fermentation terminal is 3.5; the fermented apples, pumpkins, and carrots are taken out and used for processing fruits and vegetables fermented by lactic acid bacteria, preserved fruits and vegetables, sauces, and instant s...

Embodiment 2

[0031] Take 500 grams of fresh apples and 500 grams of carrots as raw materials. After cleaning, slice them into 1 cm thick slices, blanch them at 95°C for 2 minutes, then cool them down to 20°C, put them into a fermentation tank, add 41 grams of lactic acid bacteria starter, and ferment them with lactic acid bacteria. The agent is lactic acid bacteria starter, which is compounded by the production species of Streptococcus lactis, Lactobacillus delbrueckii, Bifidobacterium, Lactobacillus plantarum, and Lactobacillus acidophilus in a mass ratio of 3:2:1:2:1. The total number of lactic acid bacteria 10 9 -10 11 / ml, sealed fermentation, the fermentation temperature is 20°C, the fermentation period is 15 days, and the pH value of the fermentation terminal is 3.2; take out the fermented apples and carrots, and use them for processing fruit and vegetable lactic acid bacteria fermented fruit and vegetable preserves, sauces, and instant side dishes; take the rest The fermented liqui...

Embodiment 3

[0034]Take 600 grams of fresh pumpkin and 200 grams of cabbage as raw materials, wash and slice into 1 cm thick slices, blanch at 100°C for 2 minutes, then cool to 22°C, put them into a fermentation tank, add 50 grams of lactic acid bacteria starter, and lactic acid bacteria ferment The agent is lactic acid bacteria starter, which is compounded by the production species of Streptococcus lactis, Lactobacillus delbrueckii, Bifidobacterium, Lactobacillus plantarum, and Lactobacillus acidophilus in a mass ratio of 3:2:1:2:1. The total number of lactic acid bacteria 10 9 —10 11 / ml, sealed and fermented, the fermentation temperature is controlled at 22°C, the fermentation period is 13 days, and the pH value of the fermentation terminal is 3.0; the fermented pumpkin and cabbage are taken out and used for processing fruit and vegetable lactic acid bacteria fermented fruit and vegetable preserves, sauces, and instant side dishes; The rest of the fermentation liquid is fully stirred, ...

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Abstract

The invention discloses a preparing technology for mycoprotein, particularly relates to a preparing technology for preparing active mycoprotein from fermentation broth and belongs to the technical field of food processing. The preparing technology for fruit and vegetable lactic acid bacterium fermentation broth mycoprotein is characterized by comprising the following steps that fruit and vegetable raw materials are taken, cleaned, split and blanched and then are cooled to the temperature of 20-27 DEG C after blanching, an efficient lactic acid bacterium starter is inoculated, the inoculating amount is 3-5 percent that of the raw materials by weight, sealed fermentation is conducted, the fermentation temperature is 20-27 DEG C, fermentation is conducted for 7-15 days under the sealing condition, the PH value of a fermentation terminal is 3.0-3.5, fermentation solids are taken out after fermentation is completed, and preserved fruits and vegetables, source and instant side dishes are prepared; sufficient stirring, suction filtering, impurity removing, concentrating and refining are conducted on the remained fermentation broth, and active mycoprotein pasty fluid is obtained. The preparing technology has the advantages that the fermentation efficiency and the product quality are improved, meanwhile, lactic acid bacteria are in mass propagation, acid production is fast, acid resistance is high, and the living bacterium content and the mycoprotein content are high.

Description

technical field [0001] The invention relates to a preparation process of mycoprotein, in particular to a preparation process of preparing mycoprotein from a fermented liquid by using fruits and vegetables such as apples, pumpkins, carrots, cabbage or cabbage as raw materials and fermenting them with lactic acid bacteria, which belongs to the food processing technology field. Background technique [0002] With the continuous improvement of living standards, people pay more and more attention to food safety, nutrition and their own health. Lactic acid bacteria and their fermented products have good effects on human health, and have physiological effects such as helping digestion, improving intestinal micro-ecological environment, inhibiting the growth of spoilage bacteria, synthesizing nutrients, and improving immunity. At present, lactic acid bacteria fermented foods mainly include lactic acid milk products and sauerkraut products. A small number of fermentation production e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23J1/00A23L33/00
CPCA23V2002/00A23V2200/324
Inventor 张华罗刚吴兴壮王芳芳王小鹤
Owner 辽宁省农业科学院食品与加工研究所
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