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Method for oxidizing and degrading graphite material through naphthalene degrading bacteria

A graphite material and bacterial oxidation technology, applied in the field of environmental biology, can solve the problems of expensive high-efficiency enzymes, difficulty in large-scale application, and difficulty in maintaining activity, and achieve the effects of strong bacterial viability, avoiding environmental problems, and rapid bacterial proliferation

Inactive Publication Date: 2015-11-04
NANJING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, high-efficiency enzymes are expensive and difficult to maintain activity, and it is almost impossible to use them in a natural environment for a long time, and it is difficult to apply them on a large scale

Method used

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  • Method for oxidizing and degrading graphite material through naphthalene degrading bacteria
  • Method for oxidizing and degrading graphite material through naphthalene degrading bacteria
  • Method for oxidizing and degrading graphite material through naphthalene degrading bacteria

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Experimental program
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Effect test

Embodiment 1

[0035] A kind of method of using naphthalene degrading bacteria to oxidize and degrade graphite material of the present invention, concrete implementation method is as follows:

[0036] The first step is to prepare inorganic salt medium. Weigh 12.5g (NH 4 ) 2 · SO 4 ,0.1g CaCl 2 ,7.5g KH 2 PO 4 ,5g NaNO 3 ,20g Na 2 HPO 4 and 50mL trace element solution, dissolved in 5L tap water. 1L trace element solution contains 0.083g KI, 0.3g H 2 BO 3 ,0.0025g ZnSO 4 ·7H 2 O, 0.0025g Na 2 MoO 4 2H 2 O, 0.0025g MnSO 4 4H 2 O and 0.0025g CuSO 4 ·5H 2 O.

[0037] In the second step, the above-mentioned inorganic salt culture medium is loaded into a 5L fermenter, and sterilized by steam. Weigh 10 g of naphthalene, dissolve it with 50 mL of hot ethanol, and quickly pour it into the stirring inorganic salt medium to prepare a naphthalene / inorganic salt medium.

[0038] In the third step, add 5% naphthalene-degrading bacteria solution to the naphthalene / inorganic salt medium...

Embodiment 2

[0046] A kind of method of using naphthalene degrading bacteria to oxidize and degrade graphite material of the present invention, concrete implementation method is as follows:

[0047] In the first step, weigh 0.3g (NH 4 ) 2 · SO 4 ,0.004g CaCl 2 ,0.3g KH 2 PO 4 ,0.2g NaNO 3 ,0.8g Na 2 HPO 4 , dissolved in 200mL tap water, put into a 1L Erlenmeyer flask and mixed with 2mL trace element solution. 1L trace element solution contains 0.083g KI, 0.3g H 2 BO 3 ,0.0025g ZnSO 4 ·7H 2 O, 0.0025g Na 2 MoO 4 2H 2 O, 0.0025g MnSO 4 4H 2 O and 0.0025g CuSO 4 ·5H 2 O.

[0048] In the second step, the inorganic salt culture in the first step is sterilized for 20 minutes under the condition of 120° C. in an autoclave. Add 0.4 g of naphthalene dissolved in hot ethanol to the culture medium, add 10 mL of naphthalene-degrading bacteria liquid, and culture on a shaker at 30°C and 180 rpm for 36 hours.

[0049] The third step is to take 0.1g of graphene, add 5mL of sterilized...

Embodiment 3

[0054] In the first step, weigh 0.75g (NH 4 ) 2 · SO 4 ,0.01g CaCl 2 ,0.75g KH 2 PO 4 ,0.5g NaNO 3 ,2g Na 2 HPO 4 and 5mL trace element solution, with 500mL tap water solution, placed in a 2L Erlenmeyer flask. 1L trace element solution contains 0.083g KI, 0.3g H 2 BO 3 ,0.0025g ZnSO 4 ·7H 2 O, 0.0025g Na 2 MoO 4 2H 2 O, 0.0025g MnSO 4 4H 2 O and 0.0025g CuSO 4 ·5H 2 O.

[0055] In the second step, dissolve 1 g of naphthalene in 10 mL of hot ethanol, sterilize the inorganic salt medium prepared in the first step, insert 20 mL of naphthalene-degrading bacteria liquid, and cultivate it on a shaking table at 35°C and 200 rpm 24h.

[0056] In the second step, take 0.2g of graphene oxide, add 50mL of sterilized water, and ultrasonically disperse in a water bath for 15 minutes, mix it with the naphthalene-degrading bacteria solution being cultivated, and continue to cultivate for 14 days.

[0057] The third step is to centrifuge the cultured bacterial liquid at 1...

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Abstract

The invention discloses a method for oxidizing and degrading a graphite material through naphthalene degrading bacteria, and belongs to the technical field of environmental biology. The method includes the steps that the naphthalene degrading bacteria are inoculated in an inorganic salt culture medium containing naphthalene; the bacteria and the graphite material are mixed to be co-cultured after the bacteria multiply; the graphite material acting on the bacteria is taken out 3 days to 14 days later; and a product of the graphite material oxidized by the bacteria is obtained after purifying and drying are conducted. According to the method, the graphite material is oxidized and degraded through the naphthalene degrading bacteria, and the method is gentle and controllable, has high environment compatibility, can be used for oxidizing graphite or graphene to prepare oxidized graphite, and can be used for degrading carbon nanometer materials in the environment as well.

Description

technical field [0001] The invention relates to the field of environmental biotechnology, in particular to a method for degrading bacterial graphite oxide and graphene by using naphthalene and degrading graphene oxide. Background technique [0002] Today, graphite materials play an important role in various scientific fields. Graphene is made of carbon atoms by sp 2 The hybrid composition is a two-dimensional material with a thickness in the nanometer scale, which has the characteristics of high mechanical strength, high thermal conductivity, and high specific surface area. Therefore, graphene is an important candidate material in the preparation of batteries, supercapacitors, and composite materials. Graphene oxide is another important member of the graphite material family. It not only has a wide range of applications in photocatalysis, but also expands the scope of use of graphene, because it can be stably dispersed in various solvents and used as a precursor of graphen...

Claims

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Application Information

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IPC IPC(8): A62D3/02A62D101/40
Inventor 孙东平刘林朱春林杨加志范孟孟黄洋陈春涛
Owner NANJING UNIV OF SCI & TECH
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