Chlorine-based gamma-biological nitrogenous fertilizer and preparation method for same
A bio-nitrogen fertilizer technology, applied in the field of chlorine-based γ-bio-nitrogen fertilizer and its preparation, can solve the problems of incomplete function and efficiency, and achieve the ability to promote nitrogen fixation and nutrient enrichment, increase yield, and prolong fertilizer efficiency The effect of action time
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[0070]The preparation and purification process of γ-polyglutamic acid is as follows: respectively inoculate Bacillus licheniformis and Bacillus subtilis into a fermenter containing sterilized medium, control the temperature at 32-35°C, cultivate for 25-35h, pH7.0, The stirring speed is 160rpm and the dissolved oxygen is continuously ventilated, and the fermentation must be 30-40 hours to obtain a fermentation liquid containing polyglutamic acid; diatomaceous earth is added to the γ-polyglutamic acid fermentation liquid, and the plate and frame filter to separate and remove the bacteria, and extract the above Add ethanol to the supernatant to precipitate with ethanol to obtain a γ-polyglutamic acid precipitate, wash the precipitate, separate the precipitate, dissolve it with deionized water to obtain a γ-polyglutamic acid solution, and spray the γ-polyglutamic acid with powder Dry to obtain gamma-polyglutamic acid powder; mix two bacteria-containing fermentation broths to prepar...
Embodiment 1
[0098] Example 1: 0.5 parts of gamma-polyglutamic acid; 10 parts of wheatgrass extract; 6 parts of microbial inoculum; 80 parts of urea, 10 parts of ammonium chloride; 8-20% increase in yield of rice after application, disease resistance, lodging resistance and stress resistance Strong, good quality and taste, less harmful substances.
Embodiment 2
[0099] Embodiment 2: 0.5 part of gamma-polyglutamic acid; 8 parts of wheat straw extract; 3 parts of microbial bacterial agent; The yield per mu can reach 1,770 jin, the market price per jin is 80 yuan, and the income per mu can be increased by 22,000 yuan, and the quality is better.
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