Sulphur-based gamma-biological nitrogenous fertilizer and preparation method thereof
A biological and nitrogen fertilizer technology, applied in the field of sulfur-based γ-biological nitrogen fertilizer and its preparation, can solve the problems such as incomplete function and efficiency, waste of direct economic losses, low fertilizer utilization rate, etc., and achieves the promotion of nitrogen fixation ability and enrichment. The effect of increasing the capacity of nutrients, increasing yield, and increasing the capacity to absorb nutrients
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[0068] The preparation and purification process of γ-polyglutamic acid is as follows: respectively inoculate Bacillus licheniformis and Bacillus subtilis into a fermenter containing sterilized medium, control the temperature at 32-35°C, cultivate for 25-35h, pH7.0, The stirring speed is 160rpm and the dissolved oxygen is continuously ventilated, and the fermentation must be 30-40 hours to obtain a fermentation liquid containing polyglutamic acid; diatomaceous earth is added to the γ-polyglutamic acid fermentation liquid, and the plate and frame filter to separate and remove the bacteria, and extract the above Add ethanol to the supernatant to precipitate with ethanol to obtain a γ-polyglutamic acid precipitate, wash the precipitate, separate the precipitate, dissolve it with deionized water to obtain a γ-polyglutamic acid solution, and spray the γ-polyglutamic acid with powder Dry to obtain gamma-polyglutamic acid powder; mix two bacteria-containing fermentation broths to prepa...
Embodiment 1
[0097] Example 1: 0.5 parts of gamma-polyglutamic acid; 10 parts of wheatgrass extract; 4 parts of microbial inoculum; 50 parts of urea, 26 parts of ammonium sulfate; 8-20% increase in rice production after application, strong disease resistance, lodging resistance and stress resistance , good quality and taste, lower harmful substances.
Embodiment 2
[0098] Embodiment 2: 0.5 part of gamma-polyglutamic acid; 8 parts of wheat straw extract; 6 parts of microbial inoculum; 45 parts of urea, 25 parts of ammonium sulfate; the general output of blueberry is 1500 jins per mu, and the yield is increased by 20% after application. The output can reach 1,800 jin, and the market price per jin is 80 yuan, which can increase the income by 24,000 yuan per mu, and the quality is better.
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