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A kind of saprophytic fungus and its application in Melaleuca pagoda

A technology of raw rotting mold and Melaleuca, which is applied in the fields of application, biocide, microorganisms, etc., can solve the problem of sharp reduction of wild Melaleuca resources, and achieve the effect of improving the survival rate of transplanting

Inactive Publication Date: 2017-11-03
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the extraction of huperzine A mainly depends on wild resources, and the extraction rate is only 0.006%-0.1%. Therefore, a large amount of market demand, limited natural resources and restrictions on its own growth and reproduction have caused a sharp decline in wild Melaleuca resources. Therefore, it is imminent to explore how to promote the growth of Melaleuca

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The wild plants of Melaleuca were transplanted into the sphagnum moss substrate for cultivation. The culture temperature is 25°C±2°C, the light intensity is 2000 Lux, and the photoperiod is 16 hours. After 2 months, the transplanting survival rate of Melaleuca pagoda was 50%.

Embodiment 2

[0026] Wash the culture medium of the tissue culture seedlings of Melaleuca pagoda, and then transplant them into the sphagnum moss medium for cultivation. The culture temperature is 25°C±2°C, the light intensity is 2000 Lux, and the photoperiod is 16 hours. After 2 months, the transplanting survival rate of Melaleuca was 45%.

Embodiment 3

[0028] (1) Isolation of endophytic fungi from Melaleuca rhizosphere

[0029] Take the fresh roots of wild Melaleuca papaya, sterilize the surface with 0.1% mercuric chloride solution, take the root hair area under the dissecting microscope, and then connect it to MS solid medium for cultivation, the cultivation temperature is 25°C±2°C, the light intensity It is 2000Lux, photoperiod 16 hours; One week later, carry out surface sterilization with 5% sodium hypochlorite solution again; Then place it on the PDA solid medium (200g of potatoes, 20g of glucose) to cultivate; After cultivating for 30 days, obtain endophyte mycelium Then through the PDA solid medium, the pure culture of the endophyte is obtained by using the plate streaking method to obtain the pure culture;

[0030] (2) Preparation of endophyte inactivated mycelia

[0031] The pure culture of endophytic bacteria is fermented and cultivated with PDA liquid medium. The conditions of fermentation and culture are shake fl...

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PUM

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Abstract

The invention discloses a Pythium sp. in Melaleuca pagoda and its application. The classification of the Pythium is Pythium sp.. Unit address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, preservation date: June 10, 2015, preservation number: CGMCC10770, the Pythium was fermented to obtain hyphae, and then subjected to high-temperature inactivation, and the inactivated The hyphae applied to the culture medium of the Melaleuca tower can improve the transplanting survival rate of the Melaleuca tower. The method introduces the endophytic fungi unique to the Melaleuca plant into the cultivation medium, which can significantly improve the adaptability of the Melaleuca plant to the new environment, and provide a new way for the transplanting of the Melaleuca plant.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a saprophytic fungus in a Melaleuca pagoda and its application. Background technique [0002] Melaleuca (Huperzia serrata (Thunb.) Trev.) is a perennial herbaceous fern belonging to Lycopodiaceae (Lycopodiaceae) of the order Lycopodiales. Huperzine A, a secondary metabolite produced by Melaleuca, is a highly efficient and low-toxic reversible acetylcholinesterase inhibitor (AChEI), which is used as a drug for the treatment of Alzheimer's disease (AD, Alzheimer disease). It is used as a nutraceutical for intelligence in the United States. The natural reproduction ability of Melaleuca is poor, and it reproduces through spores and spores in the wild state, and the spores germinate slowly, and it takes 15-20 years from germination to maturity. Wild Melaleuca has high requirements for the growth environment. It grows in the dense forests of mountains or valleys at an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P21/00C12R1/645
Inventor 郭斌何美娜陈习何玮尉亚辉
Owner NORTHWEST UNIV