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Method for obtaining cold-resistant collard plant

A technology for kale and plants, which is applied in the field of obtaining cold-resistant mutant plants of kale, and can solve problems such as restricting the popularization and application of varieties, poor cold resistance, and freezing damage

Inactive Publication Date: 2015-11-18
ZHENJIANG SUIHAN AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the vast areas of the north, kale can only be cultivated for viewing in autumn and spring. The low temperature and freezing in winter are the main obstacles to the cultivation of kale in the north, especially the kale with wrinkled leaves. Serious, restricting the further popularization and application of varieties

Method used

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  • Method for obtaining cold-resistant collard plant

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Experimental program
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Effect test

Embodiment 1

[0017] The method for obtaining the cold-resistant plant of kale, the steps are as follows:

[0018] (1) At the beginning of August, kale is sown and raised, and nutrient bowls or flowerpots can be used to raise seedlings (it is convenient to handle seedlings and can ensure sufficient nutrient growth space), select 8-10 leaves, and grow vigorously and vigorously without pests and diseases. Seedlings 600 strain.

[0019] (2) Group setting: 6 groups were set up, wherein 5 groups were experimental groups, and the other group was a control group, with 100 plants in each group;

[0020] Experimental group: spraying diethyl sulfate (DES, mutagen) solution (pH value=7), each group concentration is respectively, A group: 0.05%; B group: 0.1%; C group: 0.15%; D group : 0.2%; Group E: 0.25%;

[0021] Control group: Spray 8-10ml of water;

[0022] It is advisable that the spraying amount of the experimental group and the control group is all moist on the whole plant leaf surface of ea...

Embodiment 2

[0028] Embodiment 2 cold resistance verification test:

[0029] The seeds harvested by the mutagenic plants (group H) and the seeds harvested by the control plants (group CK) were sown together to raise seedlings. When the two types of plants were 75-80 days old, 50 vigorous and robust plants were selected and put into Treat it in a low-temperature light incubator at -10~-5°C for 3-4 days, and then move it into a light incubator at 5-10°C to grow for about a week. The heart leaves of the control plants had different degrees of frost damage, and the leaf margins had wilting, browning and curling. Plants obtained after mutagenesis treatment did not suffer from freezing damage.

[0030] Take the heart leaf position blade of each plant of mutation group and control group (get the blade of consistent or similar position), measure respectively the content of three indexes such as malondialdehyde, peroxidase (POD) and free proline, The results are shown in Table 1.

[0031] Table ...

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Abstract

The invention relates to a method for obtaining a cold-resistant collard plant. The method comprises the following steps: sowing and raising seedlings, and selecting the robust seedlings having 8-10 leaves, growing healthily, and having no plant diseases or insect pests; spraying a mutagenic agent solution; carrying out illumination culturing for one week at 15-20 DEG C, and further spraying the mutagenic agent solution; carrying out illumination culturing for 10-12 days at 1-5 DEG C, and then carrying out illumination culturing for 3-4 days at 15-20 DEG C; selecting plant leaves with relatively small parts having brown stain, wilting and malformation, and selecting the single seedlings according to the measured cold-resistant physical signs; culturing the selected single seedlings in the open field, enabling the single seedlings to live through the winter naturally, collecting the plant leaves on the coldest day of the year, selecting the excellent single mutant plants according to the measured cold-resistant physical signs, bagging during the flowering phase, so that selfing and fruit setting are realized, and harvesting the seeds after seed pods become yellowish-brown. The single mutant plants with excellent fancy point and strong cold resistance are obtained, and the cold resistance of the mutant plants is stably inherited to the offsprings, so that the territorial scope for planting promotion of collard is expanded.

Description

technical field [0001] The invention relates to a method for obtaining cold-resistant mutant plants of kale, belonging to the technical field of plant cultivation. technical background [0002] Ornamental kale (Brassica oleraceavar.acephalaf.tricolorHort.) is a variety of Brassica oleracea species in the family Brassicaceae. It is native to the European Mediterranean to the North Sea coast. The leaves are as colorful as flowers, and the horticultural varieties are various in shape. Ornamental kale is the first choice for ornamental flowers in late autumn, winter and early spring in East China, and has high ornamental value and economic benefits. In the vast areas of the north, kale can only be cultivated for viewing in autumn and spring. The low temperature and freezing in winter are the main obstacles to the cultivation of kale in the north, especially the kale with wrinkled leaves. Serious, restricting the further popularization and application of varieties. Therefore, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06
Inventor 姚悦梅戴忠良王建华潘跃平吴国平毛忠良潘永飞张振超秦文斌肖燕孙春青马志虎孙国胜
Owner ZHENJIANG SUIHAN AGRI