Rapeseed dreg microbial fermentation feed and preparation method and application thereof

A technology of microbial fermentation and rapeseed meal, applied in the direction of animal feed, animal feed, application, etc., can solve the problems of affecting the digestion, absorption and utilization of livestock, reduce the efficiency of breeding production, reduce the palatability of feed, etc., and achieve low cost and abundant resources. , the effect of increasing protein content

Inactive Publication Date: 2015-11-18
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, if rapeseed meal is directly used in feed, there will be problems such as amino acid imbalance, toxic substances and anti-nutritional factors that endanger the health of poultry and livestock, and reduce the palatability of feed, which will affect the digestion, absorption and utilization of poultry and livestock, and reduce the Production efficiency of farming

Method used

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  • Rapeseed dreg microbial fermentation feed and preparation method and application thereof
  • Rapeseed dreg microbial fermentation feed and preparation method and application thereof
  • Rapeseed dreg microbial fermentation feed and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A kind of rapeseed meal microbial fermented feed of the present invention, its preparation method specifically comprises the following steps:

[0031] S1, Bacillus natto is cultivated into Bacillus natto bacterium liquid: Bacillus natto is inoculated in the bacillus-enriched liquid medium with the inoculum size of 1g / 100mL (the composition of the bacillus-enriched liquid medium: peptone 10g, beef powder 3g, sodium chloride 5g, pH 7.3), cultured with shaking at 37°C for 16 hours at a shaking speed of 120r / min. In step S1, the Bacillus natto is Bacillus natto L08, which is deposited in the General Microorganism Center of China Committee for the Collection of Microorganisms, and the preservation number is: CGMCCNo.5769.

[0032] Bacillus natto (Bacillusnatto) L08 is Gram-positive, the size is 0.7μm~0.8μm×2.0μm~3.0μm, the spores are oval or columnar, mesozoic or partially mesozoic, have flagella, and can move; After 2 days of growth on the sesame medium, the colony is roun...

Embodiment 2

[0039] A kind of rapeseed meal microbial fermented feed of the present invention, its preparation method specifically comprises the following steps:

[0040]S1. Cultivate Bacillus natto into Bacillus natto bacteria liquid: inoculate Bacillus natto in a bacillus-enriched liquid medium with an inoculation amount of 1g / 100mL, and culture it with shaking at 37°C for 16 hours at a shaking speed of 120r / min. The Bacillus natto in Step S1 of this embodiment is the same as that in Embodiment 1.

[0041] S2. Cultivate Lactobacillus rhamnosus into Lactobacillus rhamnosus bacterial liquid: Bacillus natto was inoculated in MRS liquid medium at an inoculation amount of 1.2 g / 100 mL, and cultured at 37° C. for 18 hours. The Lactobacillus rhamnosus in Step S2 of this embodiment is the same as that of Embodiment 1.

[0042] S3, preparing microbial fermented feed:

[0043] S3-1: Take 30g of rapeseed meal crushed through a 40-mesh sieve and 0.6g of bran in a 500mL Erlenmeyer flask, add 32mL ...

Embodiment 3

[0058] Take 20g of rapeseed meal crushed through a 40-mesh sieve and place it in a 300mL conical flask, add 32mL of distilled water, stir evenly, seal and sterilize for 15min; after cooling, disperse until the texture is fluffy to make fermentation raw materials. The fermentation raw materials are fermented in the following three ways:

[0059] A: Add 1wt% Bacillus natto and 1wt% Lactobacillus rhamnosus to the fermentation raw material at the same time, stir evenly and place it at 37°C to continue fermentation for 96 hours to complete the preparation of rapeseed meal microbial fermented feed.

[0060] B: Add 1wt% Bacillus natto to the fermentation raw material and ferment for 48 hours; then add 1wt% Lactobacillus rhamnosus, stir evenly and place it at 37°C for 96 hours to complete the rapeseed meal Preparation of microbial fermented feed.

[0061] C: add 1wt% Lactobacillus rhamnosus to the fermentation raw material and ferment for 48 hours; then add 1wt% Bacillus natto, stir ...

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Abstract

The present invention discloses a rapeseed dreg microbial fermentation feed and a preparation method and an application thereof. The rapeseed dreg microbial fermentation feed uses rapeseed dregs as fermentation raw materials and is obtained by fermentation preparation by means of bacillus natto and lactobacillus rhamnosus. The preparation method is as follows: the bacillus natto is cultured into bacillus natto bacterium liquid; the lactobacillus rhamnosus is cultured into lactobacillus rhamnosus bacterium liquid; the rapeseed dregs and water are mixed to prepare the fermentation raw materials and the bacillus natto is inoculated into the fermentation raw materials to conduct fermentation for 48 hours; and then the lactobacillus rhamnosus is inoculated to continue to ferment for 96 hours. The mixed microbial fermentation method is utilized to prepare the rapeseed dreg microbial fermentation feed which is wide in the sources of rapeseed dreg raw materials, is nutritional and health-care, improves livestock growth performance, and can be used as animal feed.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a rapeseed meal microbial fermentation feed and a preparation method and application thereof. Background technique [0002] With the improvement of living standards, the dietary structure has changed greatly, and people's consumption of meat, eggs and milk has increased year by year. As a large livestock breeding country in the world, China has increased the proportion of artificial breeding year by year, and the demand for feed is constantly increasing. However, the decline of global resources and the rapid development of modern animal husbandry and feed industry have led to the shortage and price rise of major protein feed resources such as fishmeal and soybean meal worldwide, which has brought severe challenges to the feed industry. challenge. Vigorously developing new high-efficiency and environmentally friendly green feed has important social significance an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/14A23K1/00
Inventor 王传花王远亮李珂高志勇李宗军肖萌
Owner HUNAN AGRICULTURAL UNIV
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