Extraction and purification method for Trollius chinensis polysaccharide and application thereof as tobacco humectant

A purification method, the technology of nasturtium, applied in the fields of tobacco, application, and tobacco treatment, can solve the problems of unsatisfactory humectants in cigarettes, achieve cheap and easy access to raw materials and reagents, reduce irritation and miscellaneous gases, and improve the extraction process The effect of mild conditions

Inactive Publication Date: 2015-11-18
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is: according to the deficiency of the existing extraction process of Trollius polysaccharide and the defect that the moi

Method used

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  • Extraction and purification method for Trollius chinensis polysaccharide and application thereof as tobacco humectant

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Embodiment 1

[0047] b. Soaking: add 20 times the amount of water to the nasturtium powder obtained in step a, that is, the mass ratio of the nasturtium powder and water is 1:20, and soak for 2 hours at room temperature;

[0048] c. Enzyme treatment: heat the solution of nasturtium flower soaked in step b to 40° C., add cellulase for enzymolysis treatment for 2 hours, the amount of cellulase added accounts for 1.5% of the mass of nasturtium flower powder;

[0049] d. Ultrasonic extraction: Ultrasonic extraction is performed on the Trollius flower solution after enzymolysis in step c, the extraction temperature is 60°C, the ultrasonic extraction power is 300W, and the ultrasonic time is 10 minutes;

[0050] e. Solid-liquid separation: centrifuge the extract after ultrasonic extraction in step d, and centrifuge for 15 minutes at 2°C and 8000 rpm;

[0051] f. Concentrated alcohol precipitation: Concentrate the supernatant obtained after step e centrifugation under reduced pressure to 35% of th...

Embodiment 2

[0056] b. Soaking: add 22 times the amount of water to the nasturtium powder obtained in step a, that is, the mass ratio of the nasturtium powder and water is 1:22, and soak for 3 hours at room temperature;

[0057] c. Enzyme treatment: heat the solution of nasturtium flower soaked in step b to 45° C., add cellulase for enzymatic hydrolysis treatment for 1 hour, and the amount of cellulase added accounts for 2.0% of the mass of nasturtium flower powder;

[0058] d. Ultrasonic extraction: Ultrasonic extraction is performed on the Trollius flower solution after enzymolysis in step c, the extraction temperature is 70°C, the ultrasonic extraction power is 400W, and the ultrasonic time is 13 minutes;

[0059] e. Solid-liquid separation: centrifuge the extract after ultrasonic extraction in step d, and centrifuge for 15 minutes at 4°C and 9000 rpm;

[0060] f. Concentrated alcohol precipitation: Concentrate the supernatant obtained after step e centrifugation under reduced pressure ...

Embodiment 3

[0065] b. Soaking: add 25 times the amount of water to the nasturtium powder obtained in step a, that is, the mass ratio of the nasturtium powder and water is 1:25, and then soak for 4 hours at room temperature;

[0066] c. Enzyme treatment: heat the solution of nasturtium flower soaked in step b to 54° C., add cellulase for enzymatic hydrolysis treatment for 1 hour, and the amount of cellulase added accounts for 2.5% of the mass of nasturtium flower powder;

[0067] d. Ultrasonic extraction: Ultrasonic extraction is carried out on the Trollius flower solution after the enzymolysis in step c, the extraction temperature is 80°C, the ultrasonic extraction power is 500W, and the ultrasonic time is 10 minutes;

[0068] e. Solid-liquid separation: centrifuge the extract after ultrasonic extraction in step d, and centrifuge for 10 min at 6°C and 10,000 rpm;

[0069] f. Concentrated alcohol precipitation: Concentrate the supernatant obtained after step e centrifugation under reduced ...

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Abstract

The invention discloses an extraction and purification method for Trollius chinensis polysaccharide and application thereof as a tobacco humectant. The method includes: firstly drying and crushing Trollius chinensis, adding water into the crushed Trollius chinensis powder to perform soaking; then adding an enzyme preparation to conduct enzymolysis treatment, performing ultrasonic extraction after enzymolysis, subjecting the extracted solution to centrifugal separation, and carrying out pressure reduced concentration on the obtained supernate, adding ethanol to perform alcohol precipitation standing to obtain precipitate, i.e. Trollius chinensis crude polysaccharide; preparing the crude polysaccharide into a solution, removing protein by a polyamide column, subjecting the obtained solution to concentration and alcohol precipitation, and carrying out freeze drying on the obtained precipitate, thus obtaining refined Trollius chinensis polysaccharide. The Trollius chinensis polysaccharide extracted by the method provided by the invention has high purity, and the obtained Trollius chinensis polysaccharide can be dissolved to serve as a humectants to be applied in cigarettes, can achieve good physical and sensory moisturizing effect, also can reduce the irritation and miscellaneous gas of cigarettes, and improve the mellow sense and comfortableness of cigarettes.

Description

technical field [0001] The invention relates to a method for extracting effective components in plants and its application, in particular to a method for extracting and purifying the nasturtium polysaccharide and its application as a tobacco humectant. Background technique [0002] In the production of cigarettes, it is usually necessary to add humectants to improve the physical moisture retention and processing resistance of cigarettes; at the same time, the use of humectants can keep the moisture in the smoke when cigarettes are consumed in different regions, thereby reducing the smoking process of cigarettes. The dryness and pungentness of the medium smoke ensure the smoking quality of the cigarette and ensure that the cigarette designer can convey the sense of ease, comfort and satisfaction when smoking the cigarette. [0003] At present, glycerin, propylene glycol, sorbitol and other polyhydric substances are mainly used as humectants in my country’s cigarette industry....

Claims

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Application Information

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IPC IPC(8): C08B37/00A24B15/40
Inventor 姬小明闫学斌赵华新张建业赵铭钦陶陶尤芳芳
Owner HENAN AGRICULTURAL UNIVERSITY
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