A method for preparing and detecting oleic acid from defatted Antarctic krill
An Antarctic krill and oleic acid technology, which is applied in the fields of food and chemistry to achieve the effects of low cost, simple operation and broad application prospects
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Embodiment 1
[0035] A method for preparing and detecting oleic acid from defatted Antarctic krill, comprising the following steps:
[0036] Add 100g of defatted dry Antarctic krill to 600mL of methanol, heat and reflux in a water bath at 70°C for 5 extractions, and filter after each extraction for 60 minutes. The filtrates were combined, and the methanol was removed by rotary evaporation to obtain 60 g of a paste. Add 360 mL of diethyl ether to the paste, stir to dissolve, and obtain a filtrate from the residue, evaporate the diethyl ether to obtain 15.1 g of diethyl ether extract.
[0037] Take 1.93g of diethyl ether extract, add 4mL of diethyl ether, and put it in a refrigerator at 8°C to crystallize for 18h. After crystallization, the liquid was subjected to low-temperature high-speed centrifugation at 4°C, 8000 r / min, for 10 minutes, and an orange-red oily substance was obtained after diethyl ether was evaporated. Take a drop of oily substance for MIR-ATR detection, and it is detecte...
Embodiment 2
[0040] Add 800 mL of methanol to 100 g of defatted dry Antarctic krill, heat and reflux in a water bath at 75°C for 5 extractions, and filter after each extraction for 60 minutes. The filtrates were combined, and the methanol was removed by rotary evaporation to obtain 60.9 g of a paste. Add 488 mL of ether to the paste to obtain 15.3 g of ether extract. Take 1.97g of diethyl ether extract, add 6mL of diethyl ether, and put it in a refrigerator at 8°C for 24h to crystallize. After crystallization, the liquid was centrifuged at low temperature and high speed at 4°C, 8000 r / min for 15 minutes, and the orange-red oil was obtained after diethyl ether was evaporated. Take a drop of oil for MIR-ATR detection, and analyze the substance as oleic acid, such as figure 2 shown. MIR-ATR detection condition is resolution 4cm -1 , scan time 16s, mid-infrared light source, KBr beam splitter.
[0041] The weight of the orange-red oily substance was 1.12 g. Through calculation, 8.70 g o...
Embodiment 3
[0043] Add 100g of defatted dry Antarctic krill to 1000mL of methanol, heat and reflux in a water bath at 80°C for extraction twice, and filter after each extraction for 60 minutes. The filtrates were combined, and the methanol was removed by rotary evaporation to obtain 61 g of a paste. Add 660 mL of ether to the paste to obtain 15.3 g of ether extract. Take 1.96g of diethyl ether extract, add 8mL of diethyl ether, and put it in a 6°C refrigerator to crystallize for 36h. After crystallization, the liquid was centrifuged at low temperature and high speed under the conditions of 4° C., 8000 r / min, and 30 min. After diethyl ether was evaporated, an orange-red oily substance was obtained. Take a drop of oil for MIR-ATR detection, and detect that the substance is oleic acid, such as image 3 shown. MIR-ATR detection condition is resolution 4cm -1 , scan time 16s, mid-infrared light source, KBr beam splitter.
[0044] The weight of the orange-red oily substance was 1.13 g. Th...
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