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Method for carrying out enzymatic fibroin functional modification based on grafted polypeptide

A functional silk fibroin technology, which is applied in biochemical fiber treatment, textile and papermaking, fiber treatment, etc., can solve the problems such as the efficiency of functional modification of silk fibroin protein needs to be improved, and the accessibility can be reduced, so as to achieve functional and biological Good compatibility, improved efficiency, and low water solubility

Active Publication Date: 2015-11-18
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that because the tyrosine residues in silk fibroin are distributed in the macromolecular chain composed of alanine and glycine with strong hydrophobicity, the possibility of reaction between tyrosinase and the phenolic hydroxyl sites on silk fibroin is reduced. Therefore, the efficiency of functional modification of silk fibroin by tyrosinase needs to be improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Carry out functional modification of silk fabrics with the method mentioned in the present invention;

[0021] (1) Laccase / TEMPO system treatment of silk fibroin: soak silk fabrics in laccase / TEMPO system, wherein laccase 8U / ml, TEMPO2g / L, at 30 ℃, pH4.5 conditions for 12 hours, and then The sample was taken out and washed twice with water at room temperature;

[0022] (2) Polypeptide grafting on the surface of silk fibroin: the silk fabric after step (1) is immersed in the polypeptide solution of 0.5g / L (polypeptide structure: Gly-Tyr-Gly-Lys-Gly-Tyr-Gly), in Treat at 30°C and pH6.5 for 8 hours, then take out the sample and wash it twice at room temperature;

[0023] (3) enzymatic chitosan grafting on the surface of silk: the silk fabric after step (2) is immersed in a solution composed of 10U / ml tyrosinase and 4g / L chitosan, at 30 ℃, Treat at pH 6.5 for 24 hours, then take out the sample and wash it twice at room temperature;

[0024] Sample 1: untreated as it is; ...

Embodiment 2

[0029] Carry out functional modification of silk fibroin film with the method mentioned in the present invention;

[0030] (1) Treatment of silk fibroin with laccase / TEMPO system: soak the silk fibroin film after alcoholization treatment in laccase / TEMPO system, in which laccase 10U / ml, TEMPO 3g / L, 12 Hours, after the treatment, the sample was washed twice with water at room temperature;

[0031] (2) Polypeptide grafting on the surface of silk fibroin: immerse the silk fibroin film treated in step (1) in a 1g / L polypeptide solution (polypeptide structure: Lys-Gly-Tyr-Gly-Gly-Tyr-Gly-Lys) , treated at 30°C and pH 6.5 for 10 hours, and washed the sample twice with water at room temperature after treatment;

[0032] (3) Grafting of polylysine enzymatically on the surface of silk fibroin: soak the silk film treated in step (2) in the solution of tyrosinase and ε-polylysine, wherein tyrosinase 15U / ml, ε-polylysine 5g / L, treated at 30°C and pH6.5 for 12 hours, then took out the s...

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Abstract

The invention discloses a method for carrying out enzymatic fibroin functional modification based on grafted polypeptide. Serine in silk fibroin is catalytically oxidized through an enzyme to generate reactive aldehyde, and is grafted with polypeptide containing tyrosine; the number of tyrosine residues on the fibroin surface is increased; subsequent tyrosinase catalytic fibroin is promoted to react with a functional amino compound; the enzymatic modification efficiency of silk fibroin is improved; and a multifunctional fibroin material is built. The method comprises the following specific steps: (1) treating the fibroin material in a laccase / 4-oxo-2,2,6,6-tetramethyl piperidine-1-oxyl (TEMPO) system; (2) grafting the polypeptide containing tyrosine on the surface of the fibroin material; and (3) reacting the tyrosinase catalytic fibroin with the functional amino compound. Compared with a traditional chemical cross-linking method for preparing a functional fibroin material, the method has the advantages of high enzyme catalysis efficiency and mild enzyme treatment condition; and the performance of the fibroin material is obviously improved.

Description

technical field [0001] The invention relates to a method for functional modification of silk fibroin based on grafted polypeptides, in particular to a method for grafting polypeptides containing tyrosine on the surface of silk fibroin to promote tyrosinase to catalyze silk fibroin and functional modification. The invention relates to an amino compound reaction, a method for realizing enzymatic functional modification of silk fibroin materials, and belongs to the field of textile biotechnology. Background technique [0002] Silk materials include silk films, silk scaffolds and silk fabrics made of silk fibroin. Silk fibroin has good biocompatibility, and the regenerated silk fibroin material processed from it is widely used in the fields of medicine and tissue engineering. In recent years, in order to expand the application field of silk fibroin materials, relevant researches have been carried out on the functional modification of silk fibroin at home and abroad. Among them...

Claims

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Application Information

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IPC IPC(8): D06M16/00D06M15/15D06M15/03C08J7/12D06M101/10C08L89/00
Inventor 王平袁久刚王树根王强范雪荣
Owner JIANGNAN UNIV
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