Method for carrying out enzymatic fibroin functional modification based on grafted polypeptide
A functional silk fibroin technology, which is applied in biochemical fiber treatment, textile and papermaking, fiber treatment, etc., can solve the problems such as the efficiency of functional modification of silk fibroin protein needs to be improved, and the accessibility can be reduced, so as to achieve functional and biological Good compatibility, improved efficiency, and low water solubility
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Example Embodiment
[0019] Example 1
[0020] Carry out functional modification of silk fabric by the method mentioned in the present invention;
[0021] (1) Laccase / TEMPO system treatment of silk fibroin: The silk fabric is immersed in the laccase / TEMPO system, in which laccase 8U / ml, TEMPO 2g / L, treated at 30℃, pH4.5 for 12 hours, and then The sample is taken out and washed twice in water at room temperature;
[0022] (2) Polypeptide grafting on silk fibroin surface: immerse the silk fabric treated in step (1) in 0.5g / L polypeptide solution (polypeptide structure: Gly-Tyr-Gly-Lys-Gly-Tyr-Gly), Treat for 8 hours at 30°C and pH 6.5, then take out the sample and wash it with water at room temperature twice;
[0023] (3) Grafting enzymatic chitosan on the surface of silk fibroin: immerse the silk fabric treated in step (2) in a solution composed of 10U / ml tyrosinase and 4g / L chitosan at 30°C, Treat for 24 hours at pH 6.5, then take out the sample and wash it twice at room temperature;
[0024] Sample 1: U...
Example Embodiment
[0028] Example 2
[0029] Carry out functional modification of silk fibroin membrane by the method mentioned in the present invention;
[0030] (1) Laccase / TEMPO system treatment of silk fibroin: the silk fibroin film after alcoholization treatment is immersed in the laccase / TEMPO system, in which laccase is 10U / ml, TEMPO3g / L, and treated at 30℃ and pH5. Hours, wash the sample twice at room temperature after treatment;
[0031] (2) Polypeptide grafting on silk fibroin surface: immerse the silk fibroin membrane treated in step (1) in 1g / L polypeptide solution (polypeptide structure: Lys-Gly-Tyr-Gly-Gly-Tyr-Gly-Lys) , Treat for 10 hours at 30°C and pH 6.5, then wash the sample twice at room temperature after treatment;
[0032] (3) Enzymatic grafting of polylysine on the surface of silk fibroin: the silk fibroin membrane treated in step (2) is immersed in a solution of tyrosinase and ε-polylysine, wherein tyrosinase 15U / ml, ε-polylysine 5g / L, treated at 30°C and pH6.5 for 12 hours, t...
PUM
Property | Measurement | Unit |
---|---|---|
Breaking strength | aaaaa | aaaaa |
Breaking strength | aaaaa | aaaaa |
Breaking strength | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap