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Method for preparing polysaccharide-rich extract from papaya seed coat

A polysaccharide extraction and papaya seed technology is applied in the field of preparation of the papaya seed coat rich in polysaccharide extract, can solve the problems of application, and there is no preparation method for the papaya seed coat, and achieves wide application, low equipment investment and mild extraction conditions. Effect

Inactive Publication Date: 2015-11-25
HENAN YONGLONG MEDICINE & FOOD TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, the papaya seed coat is rich in water-soluble polysaccharides, which have not been discovered so far, and there is no preparation method and application of the water-soluble extract from the papaya seed coat.

Method used

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  • Method for preparing polysaccharide-rich extract from papaya seed coat

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Extraction of papaya seed coat rich in polysaccharide extract under acidic conditions at room temperature

[0025] Take 200g of papaya seeds, adjust the pH value of the water to 3 by adding sulfuric acid, add 200g of papaya seeds into 1000mL of water with adjusted pH value, stir at room temperature (25°C) at 500 rpm for 10min, filter the aqueous solution, and filter Add papaya seeds into 1000mL water with a pH value of 3, stir at 500 rpm for 10min, then filter to obtain an aqueous solution, collect it and combine it with the aqueous solution obtained from the first extraction, repeat the above steps of extraction and filtration twice, and finally collect the water-soluble extract 4000mL, freeze-dry the obtained water-soluble extract at a temperature of -55°C and a pressure of -0.04Mpa to obtain 12g of the polysaccharide-rich extract of the papaya seed coat, and the yield of the polysaccharide-rich extract of the papaya seed coat is based on 200g of papaya seed...

Embodiment 2

[0026] Example 2: Extraction of Papaya Seed Coat Rich in Polysaccharide Extract under High Temperature and Alkaline Conditions

[0027] Take 50g of papaya seed peel, the pH of the aqueous solution is controlled at 12 by sodium hydroxide, add 50g of papaya seed peel to 500mL of water with adjusted pH value, heat on a magnetic heating stirrer until the temperature is 120 degrees, and stir at 1000 rpm for 10min , filtered to obtain an aqueous solution, and repeated the above extraction and separation process 3 times to obtain a 2000mL water-soluble extract solution. The aqueous solution was dried and processed at 105°C under normal pressure to obtain 2.6g of the final product papaya seed coat-rich polysaccharide extract. The yield of papaya seed coat rich in polysaccharide extract was 5.2%.

Embodiment 3

[0028] Example 3: Extraction of papaya seed coat rich in polysaccharide extract under neutral conditions at room temperature

[0029] Take 100g of papaya seeds, add 500mL of water without adjusting the pH value, stir at room temperature (25°C) at 1000 rpm for 5 minutes, then stop, separate the water-soluble extract, filter and separate the obtained papaya seeds, and repeat the above extraction and separation steps Three times, the obtained water-soluble extract solution was collected and dried at a temperature of -55°C and a pressure of -0.04Mpa to obtain 6.5g of a water-soluble extract of papaya seed coat, with a yield of 6.5% based on papaya seeds.

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Abstract

The invention relates to a method for preparing polysaccharide-rich extract from papaya seed coat. The method comprises steps as follows: papaya seeds are added to water, stirred and subjected to extraction, an aqueous solution is obtained and subjected to centrifugal separation, the extracted papaya seeds are added to water and extracted repeatedly, the separation step is repeated, a total aqueous solution is obtained, and the polysaccharide-rich extract can be obtained through drying and water removal. Compared with a conventional extraction method, the method doesn't need an organic solution and is simple in process, environment-friendly and pollution-free; the extraction temperature is mild, the papaya seeds are not required to be smashed, the activity of the papaya seeds after extraction is kept, and follow-up planting, application and development are guaranteed; the aqueous solution after extraction is rich in polysaccharide, the application is wide, and the polysaccharide-rich extract can be used for food, medicine, cosmetic, feed or antioxidant additives; the equipment investment is small, and the method is suitable for industrial mass production.

Description

technical field [0001] The invention belongs to the technical field of comprehensive development and utilization of plant natural products, and in particular relates to a preparation method of papaya seed coat-rich polysaccharide extract that can extract active ingredients without organic solvent desolvation treatment. Background technique [0002] Papaya is the fruit of the Rosaceae papaya plant. The papaya fruit is warm and sour in taste. It has the effects of relaxing tendons and activating blood, calming the liver and stomach, dispelling dampness and relieving pain. Traditional Chinese medicine believes that papaya can remove dampness and numbness, and is an important medicine for treating chronic wind and stubborn numbness. Modern pharmacological research has proved that papaya contains active ingredients such as saponins, flavonoids, malic acid, citric acid, tartaric acid, vitamin C, tannins, pectin, etc. It is commonly used clinically to treat rheumatoid arthritis, so...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 刘华敏庞会利谷令彪秦广雍
Owner HENAN YONGLONG MEDICINE & FOOD TECH CO LTD
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