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Method for obtaining regenerated plants of stevia rebaudiana Bertoni

A culture method and plant regeneration technology, applied in the biological field, can solve problems such as unreported, achieve the effect of optimizing the regeneration system and shortening the breeding cycle

Active Publication Date: 2015-12-02
ANHUI SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, people are concerned about the aseptic propagation system of stevia rebaudiana, the selection of medium and culture conditions, the induction and differentiation of callus, the differentiation of buds and subculture, the culture of shoot tips, the culture of rooting and the transplanting of tissue cultured seedlings, etc. A relatively comprehensive and systematic research has been done on all aspects, but there is no report on the research on the in vitro culture of stevia anthers.

Method used

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  • Method for obtaining regenerated plants of stevia rebaudiana Bertoni

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Embodiment 1

[0035] (1) Bud collection

[0036] The stevia rebaudiana collects flower buds with a single-nucleus and side-by-side pollen during the flowering stage; picks a diameter of approx. 2 ~ 3mm , No pests and diseases, closed flower buds with full anthers were placed in an ice box and brought back to the laboratory; it was determined by microscopic examination that the pollen development at this time was in the mononuclear marginal stage;

[0037] (2) Low-temperature pretreatment and disinfection of flower buds;

[0038] ① The collected flower buds are subjected to temperature pretreatment; the anthers are put into a ziplock bag and placed in a refrigerator at 4°C for 3 days of pretreatment;

[0039] ②After cleaning the surface dirt with detergent water, rinse with distilled water 3~4 times, soak in 75% volume percentage alcohol on the ultra-clean workbench for 20~30s, rinse with sterile water 2~3 times, and then add 1~2 Ditween 0.1%HgCl 2 Treat for 6-8 minutes, rinse 4-5 times...

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Abstract

The invention relates to a method for obtaining regenerated plants of stevia rebaudiana Bertoni, and belongs to the field of biotechnology. The method adopts anthers of stevia rebaudiana Bertoni as a material, and comprises the main steps of bud collection, low-temperature pretreatment and disinfection, callus induction culture, callus differentiation culture, adventitious bud rooting culture, and regenerated plant seedling and transplant. Through adoption of the method, a technical system of anther culture for stevia rebaudiana Bertoni is established, so that a stevia rebaudiana Bertoni plant tissue culture and plant regeneration system can be optimized, a certain substance research foundation can be laid for selective breeding of new varieties of stevia rebaudiana Bertoni, the breeding cycle of stevia rebaudiana Bertoni can be shortened, and new germplasm resources can be created for breeding of stevia rebaudiana Bertoni.

Description

technical field [0001] The invention relates to a method for obtaining regenerated plants of Stevia rebaudiana through anther culture, and belongs to the field of biotechnology. Background technique [0002] Stevia (Steviarebaudiana Bertoni), also known as "Stevia", "Sweet Grass", belongs to the genus Stevia in the family Asteraceae, native to South America Paraguay Amambay and Mbaxacayu mountains. Steviol glycosides can control blood sugar, lower blood pressure, and promote metabolism. It is also effective in treating diabetes, obesity, regulating gastric acid, and recovering from nervous fatigue. Stevia is an ideal sweetener, its leaves are rich in steviol glycosides, because of its high sweetness (350-400 times sweeter than sucrose), low calorie (about 1 / 300 of sucrose), safe and non-toxic Features. It is known as "the third healthy sugar source in the world" internationally. In 1985 and 1990, the Ministry of Health of my country approved steviol glycosides as natural...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 周玉丽舒英杰胡能兵何克勤张雪平崔广荣
Owner ANHUI SCI & TECH UNIV
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