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Preparation method for squid skin active polypeptide

A technology of active polypeptide and squid skin, which is applied in the field of preparation of squid skin active polypeptide, can solve the problems of insufficient hydrolysis of squid skin, low antioxidant activity, long production cycle, etc., and achieve easy control of the reaction process, high antioxidant activity, The effect of short production cycles

Active Publication Date: 2015-12-02
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of this preparation method are: (1) The production cycle of the whole preparation method is long; (2) The bioactive peptide is prepared by using high-pressure hot water treatment of squid skin protein, which needs to be carried out under high-pressure conditions, and the process conditions are strict and operable. Poor resistance, and squid skin hydrolysis is not sufficient, the degree of hydrolysis is low, and the antioxidant activity is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Wash the squid skin and mash it, add 0.2% sodium hydroxide solution by mass, add 30ml sodium hydroxide solution per gram of squid skin, soak for 24 hours, wash with water until the pH is neutral, and then add mass For 10% n-butanol solution, add 30ml n-butanol solution per gram of squid skin, stir for 16 hours, wash with water and drain.

[0022] (2) Add water 90 times the mass of squid skin to the drained squid skin, stir evenly, then raise the temperature to 40°C, keep it warm for 3 hours, and adjust the pH to 8 to obtain the primary enzymatic hydrolysis solution.

[0023] (3) Add compound enzyme and sodium bicarbonate to the initial enzymatic hydrolysis solution, perform ultrasonic enzymolysis at 40°C for 20 minutes, inactivate the enzyme, and cool to obtain the final enzymatic hydrolysis solution. Add 14000u of compound enzyme per gram of primary enzymolysis solution, compound enzyme is composed of enzymes in the following mass percentages: 20% alkaline protease...

Embodiment 2

[0026] (1) Wash the squid skin and mash it, add 0.5% sodium hydroxide solution by mass, add 50ml sodium hydroxide solution per gram of squid skin, soak for 30 hours, wash with water until the pH is neutral, and then add mass For 15% n-butanol solution, add 50ml n-butanol solution per gram of squid skin, stir for 24 hours, wash with water and drain.

[0027] (2) Add water 95 times the weight of the squid skin to the drained squid skin, stir evenly, then raise the temperature to 60°C, keep it warm for 5 hours, adjust the pH to 9, and obtain the primary enzymatic hydrolysis solution.

[0028] (3) Add compound enzyme and sodium bicarbonate to the initial enzymatic hydrolysis solution, perform ultrasonic enzymolysis at 50°C for 30 minutes, inactivate the enzyme, and cool to obtain the final enzymatic hydrolysis solution. Add 15000u of compound enzyme per gram of initial enzymolysis solution, the compound enzyme is composed of enzymes in the following mass percentages: 30% alkaline ...

Embodiment 3

[0031] (1) Wash the squid skin and mash it, add 0.3% sodium hydroxide solution by mass, add 40ml of sodium hydroxide solution per gram of squid skin, soak for 25 hours, wash with water until the pH is neutral, and then add mass For 12% n-butanol solution, add 40ml n-butanol solution per gram of squid skin, stir for 20 hours, wash with water and drain.

[0032] (2) Add water 92 times the mass of squid skin to the drained squid skin, stir evenly, then raise the temperature to 45°C, keep it warm for 4 hours, adjust the pH to 8.5, and obtain the primary enzymatic hydrolysis solution.

[0033] (3) Add compound enzyme and sodium bicarbonate to the initial enzymatic hydrolysis solution, perform ultrasonic enzymolysis at 45°C for 25 minutes, inactivate the enzyme, and cool to obtain the final enzymatic hydrolysis solution. Add 14500u of compound enzyme per gram of initial enzymolysis solution, the compound enzyme is composed of enzymes in the following mass percentages: 25% alkaline p...

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PUM

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Abstract

The invention discloses a preparation method for squid skin active polypeptide. The preparation method comprises the following steps: (1) cleaning squid skin, triturating the cleaned squid skin, adding a sodium hydroxide solution, soaking the squid skin for 24 to 30 h, washing the soaked squid skin with water until a pH value is 7, then adding an n-butanol solution, carrying out stirring for 16 to 24 h, washing the squid skin with water and draining the squid skin; (2) adding water accounting for 90 to 95 times of the mass of the squid skin into the drained squid skin, carrying out uniform mixing under stirring, then carrying out heating to 40 to 60 DEG C, maintaining the temperature for 3 to 5 h and then adjusting the pH value to 8 to 9 so as to obtain initial enzymatic hydrolysate; (3) adding compound enzyme and sodium bicarbonate into the initial enzymatic hydrolysate, carrying out ultrasonic enzymatic hydrolysis at 40 to 50 DEG C for 20 to 30 min and then carrying out enzyme killing and cooling so as to obtain final enzymatic hydrolysate, wherein nitrogen is continuously introduced into a chelation liquid during enzymatic hydrolysis; and (4) subjecting the final enzymatic hydrolysate to centrifugation and collecting supernatant liquid so as to obtain a squid skin active polypeptide solution. The preparation method has the advantages of simple process steps, easily controllable reaction process, good operationality, a short production period and a high hydrolysis degree of the squid skin.

Description

technical field [0001] The invention relates to the technical field of deep processing of aquatic products, in particular to a preparation method of squid skin active polypeptide. Background technique [0002] In recent years, with the rapid development of my country's pelagic squid fishing industry, squid has increasingly become my country's main marine economic molluscs and raw materials for aquatic product processing. At present, the technical content of squid processing and utilization in my country is low, and about 35% of by-products such as squid heads, feet, skins and viscera are produced in production, of which squid skins account for about 8% to 13%. [0003] The collagen content of squid skin is relatively high, accounting for about 80% of the dry weight. According to reports, the absorption rate of collagen polypeptide is almost 100%, and it has anti-ulcer, lowering blood pressure, promoting Ca 2+ Absorption and other biological activities. Therefore, it is be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 谢超林琳
Owner ZHEJIANG OCEAN UNIV
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