Detection method for quantitative analysis of 12 kinds of flavonoid substances in tobacco leaves
A quantitative analysis and detection method technology, applied in the field of analysis and detection, can solve the problems of low sensitivity and inability to detect low-content flavonoids
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0020] B. Preparation of the test solution: Accurately weigh 10.0 mg of the pretreated sample, add 1 ml of methanol-chloroform-water extraction solution and 200 μl of internal standard solution, the internal standard solution is daidzein, genistin and The aqueous solution of hesperidin, the concentration is 1.0 μ g / mL, after sonication, centrifugation, get the supernatant as the test solution;
[0021] C, preparation of reference substance solution: Accurately weigh each standard compound respectively, i.e. 12 standard samples and 3 internal standards daidzein, genistin and hesperidin, add methanol to make a solution containing 1mg per 1ml, To obtain a 1 mg / ml flavonoid single-label solution, pipette 100 μl of a 1 mg / ml low-concentration flavonoid single-label solution and a 10 mg / ml high-concentration flavonoid single-label solution into a 100ml volumetric flask, and extract the solution with a sample, namely methanol -Chloroform-water extraction solution was prepared at cons...
Embodiment 1
[0036] ——Quantitative analysis of tobacco leaf flavonoids in tobacco cultivar K326
[0037] Experimental materials: fresh freeze-dried K326 middle tobacco leaves at maturity.
[0038] experimental method:
[0039] Accurately weigh 10.0 mg of freeze-dried tobacco leaf sample, add 1 mL of extractant (methanol-chloroform-water, 5:2:2, volume ratio) and 200 μL of internal standard solution (1.0 μg / mL), sonicate for 30 min, centrifuge, and take The supernatant was transferred to a liquid chromatography injection vial for analysis.
[0040] Chromatographic analysis conditions: chromatographic column, WatersBEHC18 (15cm×2.1mm, 1.7μm particle size); phase A, water; phase B, acetonitrile, both phases are added with 0.1% formic acid and 0.2mmol / L ammonium acetate; fluidity Gradient, 0-1min, 10%B; 1-9min, 10%B-90%B; 9-11min, 90%B-100%B; 11-11.1min, 100%B-10%B; 11.1-13min , keep 10% B; the column temperature is 30°C, the injection volume is 2 μL, and the flow rate is 0.25mL / min.
[00...
Embodiment 2
[0050] ——Quantitative analysis of tobacco leaf flavonoids in N. rustica
[0051] Experimental material: fresh freeze-dried N. rustica Ripe middle tobacco leaves.
[0052] experimental method:
[0053] Accurately weigh 10.0 mg of freeze-dried tobacco leaf sample, add 1 mL of extractant (methanol-chloroform-water, 5:2:2, volume ratio) and 200 μL of internal standard solution (1.0 μg / mL), sonicate for 30 min, centrifuge, and take The supernatant was transferred to a liquid chromatography injection vial for analysis.
[0054] Chromatographic analysis conditions: chromatographic column, WatersBEHC18 (15cm×2.1mm, 1.7μm particle size); phase A, water; phase B, acetonitrile, both phases are added with 0.1% formic acid and 0.2mmol / L ammonium acetate; fluidity Gradient, 0-1min, 10%B; 1-9min, 10%B-90%B; 9-11min, 90%B-100%B; 11-11.1min, 100%B-10%B; 11.1-13min , keep 10% B; the column temperature is 30°C, the injection volume is 2 μL, and the flow rate is 0.25mL / min.
[0055] Mass sp...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More - R&D
- Intellectual Property
- Life Sciences
- Materials
- Tech Scout
- Unparalleled Data Quality
- Higher Quality Content
- 60% Fewer Hallucinations
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com