Nutritive matrix containing rich essential amino acid and production method and application thereof

An essential amino acid, production method technology, applied in the protein composition, application, food preparation and other directions of vegetable seeds, can solve the problems of increased surface hydrophobicity, low product added value, low utilization efficiency, etc., to achieve no pollution, base material The effect of high content of essential amino acids and low production cost

Active Publication Date: 2015-12-16
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during the oil extraction process, after the protein in peanut meal is denatured at high temperature, its structure changes, the surface hydrophobicity increases, the solubility is ext

Method used

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  • Nutritive matrix containing rich essential amino acid and production method and application thereof
  • Nutritive matrix containing rich essential amino acid and production method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0036] A method for preparing a nutritional base material rich in essential amino acids, comprising the following steps:

[0037] (1) Preparation of peanut meal koji: Take peanut meal, add 1.0 times the weight of peanut meal with deionized water and mix evenly, sterilize the evenly mixed wet peanut meal at 121°C for 10 minutes, then cool to room temperature, then put Mix 0.05% koji essence and 0.5% glucose by weight of the sterilized peanut meal evenly, add to the cooled sterilized peanut meal, and stir evenly. Fermenting for 38 hours at a humidity of 90% and a temperature of 30° C. to obtain peanut meal fermented koji material;

[0038] (2) Controlled enzymolysis of peanut meal fermented koji: get 1 part of peanut meal fermented koji and 8 parts of deionized water by weight and mix evenly, add 0.24% trypsin and 0.36% alkaline protease of peanut meal koji quality , then enzymolysis at 55°C, when the degree of hydrolysis reaches 20%, add absolute ethanol, so that the ethanol c...

Embodiment 2

[0041] A method for preparing a nutritional base material rich in essential amino acids, comprising the following steps:

[0042] (1) Preparation of peanut meal koji material: take peanut meal, add 0.5 times the weight of peanut meal in deionized water and mix evenly, sterilize the evenly mixed wet peanut meal at 121°C for 10 minutes, then cool to room temperature, then put Mix 0.1% koji essence and 0.2% glucose by weight of the sterilized peanut meal evenly, add to the cooled sterilized peanut meal, and stir evenly. Ferment for 36 hours at a humidity of 95% and a temperature of 32°C to obtain peanut meal fermented koji material;

[0043] (2) Controlled enzymolysis of peanut meal fermented koji: take 1 part of peanut meal fermented koji and 5 parts of deionized water by weight and mix evenly, add 0.06% trypsin and 0.24% alkaline protease of peanut meal koji quality , then enzymolysis at 50°C, when the degree of hydrolysis reaches 15%, add absolute ethanol, so that the ethanol...

Embodiment 3

[0046] A method for preparing a nutritional base material rich in essential amino acids, comprising the following steps:

[0047] (1) Preparation of peanut meal koji: Take peanut meal, add 0.8 times the weight of peanut meal in deionized water and mix evenly, sterilize the evenly mixed moist peanut meal at 121°C for 10 minutes, then cool to room temperature, then put Mix 0.07% koji essence and 0.4% glucose by weight of the sterilized peanut meal evenly, add to the cooled sterilized peanut meal, and stir evenly. Ferment for 40 hours at a humidity of 92% and a temperature of 28°C to obtain peanut meal fermented koji material;

[0048] (2) Controlled enzymolysis of peanut meal fermented koji: take 1 part of peanut meal fermented koji and 7 parts of deionized water by weight and mix evenly, add 0.15% trypsin and 0.35% alkaline protease of peanut meal koji quality , then enzymolysis at 60°C, when the degree of hydrolysis reaches 18%, add absolute ethanol, so that the ethanol conte...

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Abstract

The invention discloses a nutritive matrix containing rich essential amino acid and a production method and application thereof. The production method includes the steps that aspergillus and glucose are inoculated to sterilized peanut meal to be fermented so as to obtain peanut meal fermented koji; protease is added into the fermented koji for control enzymolysis, ethyl alcohol is then added until the content reaches 70-90%, and supernate obtained by constant-speed stirring and centrifugal separation is peanut meal enzymatic hydrolysate containing rich essential amino acid; after enzymatic hydrolysate is concentrated, active dry yeast is added, the mixture passes through an ultrafiltration membrane, beta-cyclodextrin and citric acid are added into permeating liquid, then spraying drying is performed, and the nutritive matrix containing rich essential amino acid is obtained. A high-concentration alcohol deposition method and a membrane separation method are combined, through high-concentration ethyl alcohol treatment, necessary components high in essential amino acid are gathered, and then in combination with membrane separation, polypeptide and other substances are obtained, wherein the high molecular weight of polypeptide and other substances is below 5000 Da; the purposes of efficiently separating target substances and gathering essential amino acid are achieved, and the technological process is simplified.

Description

technical field [0001] The invention relates to a nutritional base material rich in essential amino acids, its production method and application. Background technique [0002] Amino acid is an important component of all cells and tissues in the human body, and is closely related to the growth and development of adolescents, the prenatal and postnatal care of pregnant and lying-in women, and the health and longevity of the elderly. There are only more than 20 amino acids that make up human protein, most of which can be directly synthesized in the human body or transformed from other amino acids, and a few amino acids cannot be synthesized in the human body or the synthesis speed is slow and must be supplied by food protein. Amino acids are commonly called essential amino acids in nutrition, namely isoleucine, leucine, lysine, methionine, phenylalanine, threonine, valine and tryptophan. [0003] Survey statistics show that the per capita protein intake of Chinese residents is...

Claims

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Application Information

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IPC IPC(8): A23J1/14A23J3/14A23J3/34A23L1/305
CPCY02P60/87
Inventor 苏国万赵谋明张佳男赵容钟赵强忠
Owner SOUTH CHINA UNIV OF TECH
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