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Composition, stabilizer, application of stabilizer, preservation and preparation methods of thrombin, thrombin preparation and kit

A composition and thrombin technology, which are applied in the directions of medical preparations with non-active ingredients, non-active ingredients of polymer compounds, and medical preparations containing active ingredients, etc., can solve the problem of reduced enzyme activity, poor stability, and inconvenience for patients to use And other issues

Active Publication Date: 2015-12-16
BEIJING MDC NEW SPRING MEDICAL DEVICES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This has the following problems: first, it is difficult to preserve at room temperature, which will reduce the enzyme activity; secondly, it is inconvenient for patients to use
However, thrombin, like most enzymes, has poor stability, and its aqueous solution will be completely inactivated after being stored at room temperature for 24 days. If no protective agent or stabilizer is added, or its formula is not suitable, thrombin is used as a hemostatic agent Or make diagnostic reagents, its product quality is all difficult to guarantee, so it is necessary to develop a kind of stabilizer, to enhance the stability of thrombin, thereby improve the stability of product quality with thrombin as the main component

Method used

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  • Composition, stabilizer, application of stabilizer, preservation and preparation methods of thrombin, thrombin preparation and kit
  • Composition, stabilizer, application of stabilizer, preservation and preparation methods of thrombin, thrombin preparation and kit
  • Composition, stabilizer, application of stabilizer, preservation and preparation methods of thrombin, thrombin preparation and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Example 1 Preparation of the composition

[0068] Accurately weigh 40g trehalose, 5g glycine, 5g sodium chloride, 5g bovine serum albumin, 1g sodium azide and 0.1g sodium thiosulfate, and mix.

Embodiment 2

[0069] Example 2 Preparation of composition

[0070] Accurately weigh 70g of trehalose, 15g of glycine, 7.5g of sodium chloride, 7.5g of bovine serum albumin, 3g of sodium azide and 0.15g of sodium thiosulfate, and mix.

Embodiment 3

[0071] Example 3 Preparation of the composition

[0072] Accurately weigh 80 g of trehalose, 10 g of glycine, 8 g of sodium chloride, 6 g of bovine serum albumin, 4 g of sodium azide and 0.1 g of sodium thiosulfate, and mix.

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Abstract

The invention relates to the field of biological technology, in particular to composition, a stabilizer, an application of the stabilizer, preservation and preparation methods of thrombin, a thrombin preparation and a kit. The composition and the stabilizer can guarantee that the enzyme activity of liquid thrombin is not lost under a room temperature condition in two years. Besides, the liquid thrombin prepared with the method can be used for preparing a thrombin hemostatic and manufacturing a kit for testing the content of FIB (fibrinogen), has wide and profound market prospect and can create considerable economic benefits and good social benefits.

Description

Technical field [0001] The invention relates to the field of biotechnology, in particular to a composition, a stabilizer and its use, a storage method and a preparation method of thrombin, a thrombin preparation, and a kit. Background technique [0002] Thrombin, a proteolytic enzyme formed by activation of thrombin precursor (an essential component in plasma), can catalyze fibrinogen into fibrin to promote blood coagulation, and is used for local hemostasis of capillary bleeding and surgical operations After the tissue heals. [0003] Thrombin can directly act on fibrinogen in the blood to promote its conversion into fibrin. After topical application, the blood acting on the surface of the lesion quickly forms a stable clot, which is used to control capillary and venous bleeding, or as an adhesive or fixative for skin and tissue grafts. Other effects of thrombin on the blood coagulation system include inducing platelet aggregation and secondary release reactions. Thrombin is su...

Claims

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Application Information

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IPC IPC(8): A61K47/42A61K47/36A61K47/18A61K47/02A61K38/48
Inventor 胡军胡琦杨军艳
Owner BEIJING MDC NEW SPRING MEDICAL DEVICES
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