Single-molecule sequencing chip

Abstract

The invention provides a single-molecule sequencing chip, which comprises a substrate and a base layer which is arranged in a mode of being laminated with the substrate, wherein the substrate comprises a first surface and a second surface which are oppositely arranged; a reaction tank array which is formed by a plurality of runners is arranged on the first surface of the substrate in a spacing mode; two opposite sidewalls of each runner extend in the length direction of the runner and are intersected at two ends of the runner to form two conical tail ends having included angles; fluid input holes and fluid output holes which are communicated with the second surface of the substrate are respectively formed in the surfaces of the two conical tail ends; the base layer comprises a transparent base and a spacing layer which is arranged on the surface of the transparent base; and the spacing layer is contacted with the first surface of the substrate and the spacing layer, corresponding to the locations of the runners, is formed with corrosion grooves. The chip is good in flow field distribution, the base in the chip is low in deformation rate and fluid in the chip is thorough in flushing switching.

Description

technical field [0001] The invention relates to the technical field of DNA sequencing chips, in particular to a single-molecule sequencing chip. Background technique [0002] After entering the 21st century, the completion of the Human Genome Project has had a huge impact on contemporary biological research and medical research. As far as gene sequence analysis is concerned, the focus of the post-genome era has shifted from the determination of the whole genome sequence of a single species to the comparison of individual genetic differences and inter-species genetic differences in a certain species at the level of genomic DNA sequence. Targeted gene resequencing will be the mainstream technology for clinical genetic testing in the future. Single-molecule sequencing technology is known as the third-generation sequencing technology, and its notable feature is that it can directly identify DNA fragments with high fidelity. For the next generation sequencing technology) higher...

AI Technical Summary

Problems solved by technology

However, the second-generation sequencing chip is currently the mainstream product on the market. Most of them use semiconductor nano-processing technology to obtain high-density nano-arrays. clean room to complete

In addition, in order to achieve the purpose of high-throughput sequencing, the second-generation sequencing chip usually has a large channel width. When the negative pressure pumping method is used to inject samples for biochemical reactions, there are usually problems of uneven flow field distribution of the fluid and cover glass. The problem of easy deformation of the film

Uneven distribution of the flow field will cause unclean reagent switching and affect biochemical reactions. The deformation of the cover glass will affect the quality of the chip and even affect the collection of base optical signals.

[0004] Single-molecule sequencing technology does not require high-density nanoarrays like the second-generation sequencing chip because of its low requirement on the amount of sequencing data. Therefore, the traditional second-generation sequencing chip cannot match the single-molecule sequencing technology. Therefore, it is necessary to provide a Chips for Single Molecule Sequencing

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