Preparation method and application of secondary-salinization soil remediation microbial inoculum

A technology of secondary salinization and soil remediation, which is applied in the field of preparation of secondary salinized soil remediation bacteria for facility vegetables, can solve the problem of determining the survival rate and number of surviving bacteria, limiting the application of microbial remediation machines, and being very difficult to solve. It is difficult to ensure the effectiveness and other problems, to achieve the effect of promoting microbial diversity, reducing nitrous oxide gas emissions, and reducing volume

Active Publication Date: 2015-12-16
SHANGHAI JIAO TONG UNIV
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AI Technical Summary

Problems solved by technology

The problem of large-scale secondary salinization in my country has become increasingly prominent, and the research on the treatment of nitrate nitrogen by microbial methods has broad prospects. Appropriate dosage forms can be reduced in size and transported over long distances, which severely limits the application of microbial remediation machines in the remediation of secondary salinized soils
However, this technology does not make the bacterial solution into a relatively small dosage form, which is inconvenient when applied; the transportation cost increases accordingly; the final bacterial survival rate and survival quantity are not measured, and it is difficult to guarantee the effectiveness of application

Method used

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  • Preparation method and application of secondary-salinization soil remediation microbial inoculum

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Experimental program
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Effect test

Embodiment 1

[0032] Preparation of bacteria agent for remediation of secondary salinized soil by facility vegetables:

[0033] Strain recovery: take out the Bacillus megaterium strain NCT-2 from the ‐80 °C refrigerator, inoculate it on the LB solid medium, and incubate at 35 °C for 24 hours.

[0034] Expansion cultivation: Incorporate the Bacillus megaterium strain on the LB solid medium into an Erlenmeyer flask by using an inorganic salt medium, culture it at 32°C for 18 hours, and use it as a seed solution.

[0035] Fermentation culture: the seed solution was introduced into a fermenter with 5% inoculum amount and cultured at 35° C. for 24 hours to obtain a bacterial solution.

[0036] Screening of a single protective agent: add 3% sucrose, mannitol, 1% Twen80, 4% glycerin, skimmed milk powder, and trehalose to the bacterial solution as a single protective agent, freeze-dried thickness 5nm, cold trap temperature -40 ℃, 20pa freeze-dried for 20h, and observed the cell survival rate.

[...

Embodiment 2

[0039] Detection of nitrate-nitrogen transformation ability of facility vegetable secondary salinized soil remediation bacteria agent.

[0040] Take 1 g of the bacterial agent and add it to a liquid inorganic salt medium with a nitrate nitrogen concentration of 200mg / kg to simulate the soil to be treated, and cultivate it on a constant temperature shaker at 120r / min at 25°C, and measure the effect of the bacterial agent on nitrate nitrogen after five days. transforming ability.

[0041] AA3 flow injection analyzer was used to measure the content of nitrate nitrogen in the simulated secondary salinized soil after remediation, and the results were as follows figure 1Shown is the change of nitrate nitrogen content in the inorganic salt medium after adding 1g of bacterial agent.

[0042] The results showed that the nitrate content in the simulated secondary salinized soil decreased significantly by 28.7% after adding the remedial bacteria agent. It can be seen that the freeze-dr...

Embodiment 3

[0044] In this example, the pot planting simulation experiment restores the secondary salinized soil of small green vegetables in the facility. The type of secondary salinized soil cultivated in the test facility is sandy soil, and the content of nitrate nitrogen in the soil is 0.77g / kg.

[0045] Each pot is filled with 1.2kg of soil, the bacterial agent is applied to the surface soil at a ratio of 1:100, and 40 small green cabbage seeds are sown in each pot. State nitrogen content, after 45 days, observe the growth of small green vegetables.

[0046] The content of nitrate nitrogen in soil was determined by AA3 flow injection analyzer.

[0047] The results showed that after adding the remedial bacteria agent, the content of nitrate decreased significantly by 32.1%, and the EC value of the soil decreased by 25.7%. The remediation effect was obvious. Compared with the control group, the emergence rate of the small green vegetables increased by 32%, and the average yield increa...

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Abstract

The invention discloses a preparation method and application of a secondary-salinization soil remediation microbial inoculum, wherein the secondary-salinization soil remediation microbial inoculum consists of a freeze-dried bacillus megaterium strain fermentation broth and a protective agent; specifically, the bacillus megaterium is bacillus megaterium NCT-2 which is preserved in China General Microbiological Culture Collection Center with preservation number of CGMCC No.4698; and the protective agent is any one or any combinations of sucrose, mannitol, Tween 80, glycerol, skimmed milk powder, sorbitol, starch, L-sodium glutamate, maltodextrin, glucose and trehalose. The invention is simple in technology and low in cost, and a finished product is convenient to carry, good in performance quality and is capable of effectively reducing nitrate content in soil, increasing organic matter content in the soil and effectively improving the yield and the quality of vegetables. The freeze-dried microbial inoculum has a broad prospect in the remediation of secondary-salinization soil of greenhouse vegetables.

Description

technical field [0001] The invention relates to a restoration technology in the technical field of soil microorganisms, in particular to a preparation method and application of a microbial agent for restoration of secondary salinized soil of facility vegetables. Background technique [0002] Due to the long-term application of chemical fertilizers, especially nitrogen fertilizers in agricultural production, which exceeds the needs of plants and accumulates in plants and soil, and unreasonable soil management, the secondary salinization of soil is a dilemma faced by my country's agricultural production. On the one hand, secondary salinization of soil will lead to the accumulation of nitrate on the soil surface, which will affect the growth and yield of crops; source pollution; finally, the nitrate in the plant enters the human body, which seriously causes death, and also synthesizes carcinogenic compounds that affect human health. At present, the methods used to restore seco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04B09C1/10
Inventor 周培王大欣张丹支月娥初少华俞明磊
Owner SHANGHAI JIAO TONG UNIV
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