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Group of genes for lung cancer molecular subtyping and application thereof

A molecular typing and genetic technology, applied in the field of cancer diagnosis and molecular biology, can solve the problems of high false positive and false negative, low diagnostic efficiency, etc.

Active Publication Date: 2015-12-16
HANGZHOU YUANQING BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, carcinoembryonic antigen (CEA) is used for the detection of lung adenocarcinoma, cytokeratin 19 fragment 21-1 (CYFR21-1) is used for the detection of lung squamous cell carcinoma, and nerve-specific enolase (NSE) is used for small cell lung cancer. The diagnosis of adenocarcinoma, etc., but because of sensitivity and specificity issues, there are high false positives and false negatives, and the diagnostic efficiency is low

Method used

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  • Group of genes for lung cancer molecular subtyping and application thereof
  • Group of genes for lung cancer molecular subtyping and application thereof
  • Group of genes for lung cancer molecular subtyping and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Training set sample collection and processing:

[0074] The present invention analyzes the clinical data and biological sample data of lung cancer patients with a large sample size, including 391 cases of lung adenocarcinoma and 237 cases of lung squamous cell carcinoma, and a total of 628 cases of relevant clinical data and gene expression data of patients to construct a lung cancer gene expression profile database.

[0075] Screening of 13 specific genes:

[0076] According to the measured value of gene expression abundance, the inventors used the statistical analysis method T-test to screen out 13 genes that are closely related to the primary tumor site from more than 20,000 genes. These genes were differentially expressed in lung cancer subtypes, with statistical significance, as shown in Table 1.

[0077] Table 1: 13 Gene Sets

[0078] Gene

note

P value

COMP

Cartilage Oligomeric Matrix Protein

<0.001

FXYD3

FXYD Doma...

Embodiment 2

[0082] Validation set test:

[0083] In this example, the inventors analyzed the high-throughput sequencing data of 1130 cases of lung cancer, including 576 cases of lung adenocarcinoma and 554 cases of lung squamous cell carcinoma. The 13-gene statistical analysis model was used to discriminate the subtype of each sample, and compared with the results of clinicopathological diagnosis, the accuracy rate was 82.7%. Taking lung adenocarcinoma as a reference, the predicted sensitivity is 93.0%, and the specificity is 72.0%, see Table 2.

[0084] Table 2: Discrimination results of the 13-gene model in the validation set of 1130 cases

[0085]

Embodiment 3

[0087] Screening of 30 specific genes:

[0088] The inventors relaxed the screening criteria of the T-test to a P value of less than 0.01, and further obtained 17 additional genes. These genes were differentially expressed in lung adenocarcinoma and lung squamous cell carcinoma, and the difference was statistically significant. The 17 genes were combined with the 13 genes in Example 1 to form a set of 30 genes, as shown in Table 3.

[0089] Table 3: 17 Gene Sets

[0090] Gene

note

P value

KRT5

Keratin 5, Type II

<0.01

S100A2

S100A2

<0.01

KRT6A

Keratin 6A, Type II

<0.01

MIR205HG

MIR205 Host Gene

<0.01

KRT17

Keratin 17, Type I

<0.01

CLCA2

Chloride Channel Accessory 2

<0.01

SERPIN B3

Serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 3

<0.01

MMP12

Matrix Metallopeptidase 12

<0.01

DSG3

Desmoglein 3

<0.01

KRT6B

Keratin 6B...

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Abstract

The invention discloses a group of genes for lung cancer molecular subtyping. The group of genes for lung cancer molecular subtyping comprises thirteen genes such as a COMP gene and an FXYD3 gene. Besides, the invention further discloses a kit for lung cancer molecular subtyping and application thereof. The genes, the kit and the application thereof can facilitate differential diagnosis of lung cancer subtypes, the subtypes of lung cancers can be objectively and accurately discriminated by performing detection and joint analysis to the gene group after lung cancer samples are obtained, and thereby pertinent treatment can be performed to patients.

Description

technical field [0001] The invention relates to the fields of cancer diagnosis and molecular biology, as well as the clinical application of diagnosis technology. Specifically, the present invention relates to a group of genes used for molecular typing of lung cancer. By establishing a molecular typing method for lung cancer and detecting the expression of specific genes in lung cancer tissues, the most important subtypes of lung cancer can be identified: lung adenocarcinoma and squamous cell carcinoma of the lung. The invention also relates to a kit for completing the method for molecular typing of lung cancer. Background technique [0002] Lung cancer is one of the malignant tumors that threaten the health and life of people worldwide. The morbidity and mortality of male lung cancer accounted for the first place in malignant tumors, and the incidence rate of female lung cancer accounted for the second place in malignant tumors, second only to breast cancer, and the death...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11C40B40/06
CPCC12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/166C40B40/06
Inventor 徐清华杜祥王奇峰陈金影杨扬
Owner HANGZHOU YUANQING BIOTECH CO LTD
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