Method for recycling and comprehensively utilizing pleuromutilin waste residues

A technology for waste and resource utilization of pleurotus, which is applied in the field of resource recycling and can solve problems such as increased floor space, management confusion, and hidden safety hazards.

Active Publication Date: 2016-01-06
山东胜利生物工程有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, various enterprises dry and seal the waste fungus residue, which not only increases the area occupied, but also increases the possibility of secondary pollution, and the cost is greatly increased; there are also companies that sell the waste fungus residue to other private companies for drying, processing and composting Fermentation to make organic fertilizer, but the management of this method is chaotic; some private companies directly make the fungus residue into feed additives, which has many potential safety hazards

Method used

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  • Method for recycling and comprehensively utilizing pleuromutilin waste residues

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] 1) Weigh 800g of pleuromutilin waste bacteria residue with a water content of less than 2%, add 4Kg of water to the pleuromutilin waste bacteria residue, let stand for 15 minutes, and then add 4mol / L phosphoric acid to adjust the pH to 5.0 to obtain Bacterial residue

[0043] 2) Add 0.08 g of composite enzyme to the bacterial residue liquid obtained in 1), the composite enzyme is composed of cellulase: xylanase: β-glucanase in a mass ratio of 1:1:1, 30°C enzyme Decompose for 60 minutes, filter to remove undecomposed residue, and the filtrate is protein nutrient solution;

[0044] 3) Take the strain and inoculate it into the seed culture medium, the inoculation amount is 1cm 2 Cultured at 25.5°C for 65h, the pH of the seed medium was 6.15, the aeration rate was 1.0vvm, and the rotation speed was 220rpm.

[0045] The bacterial species are classified and named as Clitopilus prunulus in Latin literature, and are preserved in the General Microbiology Center of the China Microbial ...

Embodiment 2

[0054] 1) Weigh 1.1Kg of pleuromutilin waste bacteria residue with a water content of less than 2%, add 5.5Kg of water to the pleuromutilin waste bacteria residue, let stand for 15min, and then add 4mol / L phosphoric acid to adjust the pH to 5.0 , Get the bacteria residue liquid;

[0055] 2) Add 0.11 g of composite enzyme to the bacterial residue liquid obtained in 1), the composite enzyme is composed of cellulase: xylanase: β-glucanase in a mass ratio of 1:1:1, 30°C enzyme Dissolve for 70 minutes, filter to remove undecomposed residue, and the filtrate is protein nutrient solution;

[0056] 3) Take the strain and inoculate it into the seed culture medium, the inoculum is 1.5cm 2 Cultured at 26°C for 68h, the pH of the seed medium is 6.2, the aeration rate is 1.1vvm, and the rotation speed is 230rpm.

[0057] The bacterial species are classified and named as Clitopilus prunulus in Latin literature, and are preserved in the General Microbiology Center of the China Microbial Culture Co...

Embodiment 3

[0066] 1) Weigh 50Kg of pleuromutilin waste fungus residue with a water content of less than 2%, add 250Kg of water to the pleuromutilin waste fungus residue, let stand for 15 minutes, and then add 4mol / L phosphoric acid to adjust the pH to 5.0 to obtain Bacterial residue

[0067] 2) Add 5 g of composite enzyme to the bacterial residue liquid obtained in 1), the composite enzyme is composed of cellulase: xylanase: β-glucanase in a mass ratio of 1:1:1, enzymatically digested at 30°C 90min, filter to remove undecomposed residue, the filtrate is protein nutrient solution;

[0068] 3) Take the strain and inoculate it into the seed culture medium, the inoculum amount is 2cm 2 Slant, cultured at 26.5℃ for 70h, the pH of the seed medium is 6.25, the aeration rate is 1.1vvm, and the rotation speed is 250rpm.

[0069] The bacterial species are classified and named as Clitopilus prunulus in Latin literature, and are preserved in the General Microbiology Center of the China Microbial Culture C...

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Abstract

The invention relates to a method for recycling and comprehensively utilizing pleuromutilin waste residues. The method includes adding the pleuromutilin waste residues with the water contents lower than 2% into water, allowing the pleuromutilin waste residues to stand still for 15 min, and adding 4 mol / L phosphate into the pleuromutilin waste residues to regulate a pH (potential of hydrogen) value of the pleuromutilin waste residues until the pH value is 5.0-6.0 so as to obtain residue liquid; adding compound enzymes into the residue liquid, carrying out enzymatic hydrolysis at the temperatures of 30 DEG C for 60-90 min, filtering the residue liquid to obtain filter liquid which is protein nutrient liquid; inoculating strains in seed cultivation media with a pH value of 6.15-6.25 under the conditions of ventilation of 1.0-1.1 vvm and the rotational speeds of 200-250 rpm, inoculating the cultivated strains in fermentation cultivation media with a pH value of 8.15-8.25 and cultivating the strains under the condition of ventilation of 0.6-1.3 vvm and the rotational speeds of 70-115 rpm at the temperatures of 24.5-26.5 DEG C for 270-300 h so as to obtain fermentation liquid. A classification name of the strains is sweet-bread mushroom, the Latin scientific name of the strains is clitopilus prunulus, the strains are preserved in the Common Microorganism Center of the China Committee for Culture Collection of Microorganisms on 8 May, 2012, and the preservation number of the strains is CGMCC No.6093. The inoculation quantity of the strains accounts for 8%-10% of the total mass of the strains and the fermentation cultivation media.

Description

Technical field [0001] The invention relates to a method for comprehensive utilization of pleuromutilin waste fungus residues, belonging to the field of resource recycling. Background technique [0002] The waste pleuromutilin residue is the residue remaining after the fermentation of pleuromutilin, which mainly includes mycelium, remaining medium, inorganic salts, metabolites and a small amount of pleuromutilin. After testing, the waste bacteria residue is rich in protein, the content can reach about 20%. In addition to protein, the bacteria residue also contains relatively rich polysaccharides, fat, starch, cellulose and other substances. According to the regulations of the National Hazardous Waste Catalogue: The waste fungus residue produced by the fermentation of pleuromutilin belongs to hazardous waste, code HW-02. According to regulations, the disposal of hazardous waste must be handed over to a qualified and formal unit for disposal. The disposal cost per ton is around 3,...

Claims

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Application Information

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IPC IPC(8): C12P15/00C12R1/645
Inventor 邱向东张铜镇何惠欣张善飞王甜
Owner 山东胜利生物工程有限公司
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