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Quality control method of lichee polyphenols and products thereof

A quality control method and lychee polyphenol technology, applied in the field of chemical detection, can solve problems such as poor reproducibility, inability to reflect the content, proportion and efficacy of components, and inability to determine the composition of extracts.

Inactive Publication Date: 2016-01-06
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are many polyphenolic components extracted, and the UV method has some defects, such as poor reproducibility, low accuracy (susceptible to interference by impurities), and the composition of the extract cannot be determined. HPLC method is used to measure 1 to 2 components in it. It also cannot reflect the relationship between the content and ratio of the overall ingredients and the efficacy of the drug, so it is necessary to establish a quality standard that can faithfully reflect its intrinsic quality

Method used

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  • Quality control method of lichee polyphenols and products thereof
  • Quality control method of lichee polyphenols and products thereof
  • Quality control method of lichee polyphenols and products thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] A litchi polyphenol provided by the present invention is a polyphenol substance extracted from litchi skin, core, and leafy twigs as raw materials, further subjected to column chromatography, extraction, and combined with HPLC semi-preparative chromatography to obtain compound 1 (-)-epicatechin, compound 2 procyanidin A2, compound 3 procyanidin B2, compound 4PC-C, compound 5B type procyanidin dimer, compound 6A type procyanidin trimer.

[0030] Its preparation method is to take litchi polyphenol extract, and the separation steps are shown in the attached figure 1 , specifically after being purified by AB-8 macroporous resin, the eluate was concentrated under reduced pressure at 40-50°C to no alcohol smell, and then extracted with petroleum ether (1:2) for 1-2 times, the petroleum ether layer was discarded, water The layers were extracted with ethyl acetate (1:3) for 1 to 3 times, the ethyl acetate layers were combined, and concentrated to dryness under reduced pressure ...

Embodiment 2

[0051] 1 Instruments and reagents

[0052] 1.1 Instrument: ACQUITYUPLCH-Class liquid chromatograph (Waters, USA); Chromatographic column: ACQUITY HSST3 (2.1×100mm; 1.8μm)

[0053] 1.2 Test drug:

[0054] 1.2.1 Reference substance: epicatechin reference substance (batch number: 878-200102, China Institute for the Control of Pharmaceutical and Biological Products); proanthocyanidin B2 reference substance (batch number: 20120525, Tianjin Jianfeng Natural Products Research and Development Co., Ltd.); proanthocyanidin A2 reference substance , epicatechin-(4β→8, 2β→O→7)-epicatechin-(4β→8)-epicatechin reference substance (prepared by Institute of Materia Medica, Guangdong Pharmaceutical College, tested by UV, IR, ESI -MS 2 , NMR spectrum confirmation, purity ≥ 98%)

[0055] 1.2.2 Test product: 10 batches of lychee peel (including 7 varieties: Heiye, Feizixiao, Nuomici, Baiwa, Guiwei, Sophora japonica, Baitangying) and extracts of 5 black leaf lychee peels at different maturity s...

Embodiment 3

[0083] 1 Instruments and reagents

[0084] 1.1 Instrument: ACQUITYUPLCH-Class liquid chromatograph (Waters, USA); Chromatographic column: ACQUITY HSST3 (2.1×100mm; 1.8μm)

[0085] 1.2 Test drug:

[0086]1.2.1 Reference substance: epicatechin reference substance (batch number: 878-200102, China Institute for the Control of Pharmaceutical and Biological Products); proanthocyanidin B2 reference substance (batch number: 20120525, Tianjin Jianfeng Natural Products Research and Development Co., Ltd.); proanthocyanidin A2 reference substance , epicatechin-(4β→8, 2β→O→7)-epicatechin-(4β→8)-epicatechin reference substance (prepared by Institute of Materia Medica, Guangdong Pharmaceutical College, tested by UV, IR, ESI -MS 2 , NMR spectrum confirmation, purity ≥ 98%).

[0087] 1.2.2 Test items: extract, lychee polyphenol microspheres, lychee polyphenol nanoemulsion, lychee polyphenol capsule, lychee polyphenol tablet.

[0088] 2 Methods and results

[0089] 2.1 Preparation of sol...

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Abstract

The invention discloses a quality control method of lichee polyphenols and products thereof, and aims to provide the lichee polyphenols with extensive use and a method capable of simultaneously qualitative and quantitative determination of the content of various effective components in the litchi polyphenols and preparations thereof and building of comprehensive evaluation and monitoring of the quality. The method comprises the steps: with lichee peel, kernels and epicormic branches with leaves as raw materials, extracting to obtain polyphenols, further carrying out column chromatography and extraction, combining with HPLC semipreparative chromatography, and separating to obtain a compound 1 (-)-epicatechin, a compound 2 procyanidin A2, a compound 3 procyanidin B2, a compound 4 epicatechin-(4beta->8,2beta->O->7)-epicatechin-(4beta->8)-epicatechin called PC-C for short, a compound 5 B-type procyanidin dimer, and a compound 6 A-type procyanidin trimer. The quality control method belongs to the technical field of chemical detection.

Description

technical field [0001] The invention discloses a quality control method of polyphenols and its products, in particular, a quality control method of litchi polyphenols and its products; it belongs to the technical field of chemical detection. Background technique [0002] Plant polyphenols are the general term for polyhydric phenolic compounds, which are widely found in vegetables, fruits, beans, tea and other plants. The unique structure of polyphenols endows it with some unique chemical properties, making it anti-oxidant, preventing heart and brain Vascular disease, anti-tumor, anti-aging and other functions are more and more widely used in the fields of medicine, health food, cosmetics and functional beverages. Plant polyphenol antioxidant extracts that have been successfully developed and utilized include grape seed extract and green tea extract. The research results show that the total phenolic content and antioxidant activity of litchi extract are very close to those o...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 邓红张蜀李焕清
Owner GUANGDONG PHARMA UNIV
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