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Application of ethyl gallate for preparing drugs for treating human colon carcinoma cell apoptosis

A technology of ethyl gallate and colon cancer, applied in the field of cell biology, to achieve the effect of low toxicity and high safety

Inactive Publication Date: 2016-01-13
QIQIHAR MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

So far, there is no report about the effect of ethyl gallate on the apoptosis of colon cancer cell Lovo and its application in medicine

Method used

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  • Application of ethyl gallate for preparing drugs for treating human colon carcinoma cell apoptosis
  • Application of ethyl gallate for preparing drugs for treating human colon carcinoma cell apoptosis
  • Application of ethyl gallate for preparing drugs for treating human colon carcinoma cell apoptosis

Examples

Experimental program
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Effect test

experiment example

[0024] Experimental example: research on the apoptosis-inducing effect of ethyl gallate of the present invention on colon cancer cell Lovo

[0025] 1.1 Experimental materials

[0026] 1.2 Cell lines: purchased from the Cell Bank of the Chinese Academy of Sciences.

[0027] 1.2 Main drugs and reagents: Ethyl gallate (provided by the Department of Natural Medicine, Qiqihar Medical College). DMEM medium (purchased from Gibco, USA); fetal bovine serum (Hyclone); DMSO (dimethyl sulfoxide) (Ameresco, USA); acridine orange (AO) (Shanghai Blue Season Technology Development Co., Ltd.); Ethidine (EB) (Dalian Free Trade Zone Lianbotai Biotechnology Co., Ltd.); AnnexinV-FITC kit (KGI Biotechnology); DNALadder, BeyoRedDNA sample buffer (6X), cell apoptosis-DNALadder extraction reagent (spin column formula) (Biyuntian). CleavedCaspase-3 antibody, CleavedCaspase-8, CleavedCaspase-9 antibody (CellSignalingTechnology); Rabbit Streptavidin-HRP (SA-HRP) series kit (Kang Wei Century).

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Abstract

The invention discloses application of ethyl gallate for preparing drugs for treating colon carcinoma cell Lovo apoptosis. Pharmacology and cell biology experiments show that the ethyl gallate can effectively promote chromatin condensation of colon carcinoma cell Lovo, plasma membrane translocation of phosphatidylserine and breakage of internal DNAs of nucleuses and can induce cell apoptosis, and the action mechanism is closely related to improvement on expression level of Caspase-3, Caspase-8 and Caspase-9 proteins in the cells. The apoptosis-inducing effect of the ethyl gallate on the colon carcinoma cell Lovo is disclosed and subjected to quantitative analysis for the first time, research and development for new drugs with anti-cancer activity from the ethyl gallate from the perspective of apoptosis are benefitted, seeking for prevention and treatment of colon carcinoma diseases is benefitted, and new thoughts are provided for treatment of various tumors.

Description

technical field [0001] The invention relates to the application of ethyl gallate in the preparation of a medicine for treating apoptosis of human colon cancer, belonging to the technical field of cell biology. Background technique [0002] Colorectal cancer is one of the most common malignant tumors of the digestive tract, and its morbidity and mortality rank third among malignant tumors in the world. With changes in human life patterns, food structure, and eating habits, the incidence and mortality of colon cancer The mortality rate is increasing year by year. Facing such a serious social problem, how to improve the survival rate of patients with colon cancer has become an important topic for scientists at home and abroad. At present, the clinical treatment of colon cancer is mainly based on surgery, but in order to enhance the therapeutic effect, it is often necessary to use traditional Chinese medicine as an adjuvant treatment in order to achieve a better therapeutic eff...

Claims

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Application Information

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IPC IPC(8): A61K31/235A61P35/00
Inventor 简白羽刘吉成韩翠艳董岩张昊
Owner QIQIHAR MEDICAL UNIVERSITY
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