Method for degrading bisphenol A through white-rot fungus crude enzyme

The technology of white rot fungi and crude enzyme solution is applied in the field of environmental remediation, which can solve the problems of large workload, low accuracy, cumbersome steps, etc., and achieve the effects of low cost, simple operation and high-efficiency catalytic ability.

Active Publication Date: 2016-01-13
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Description
  • Claims
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Problems solved by technology

[0006] The purpose of the present invention is to provide a method for degrading bisphenol A by using white rot fungus crude enzyme solution to solve the problems of reversibility, heavy workload, high cost, cumbersome steps and low accuracy in the process of BPA degradation in the prior art. Methods

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[0020] In order to make the technical means, creative features, goals and functions achieved by this patent more clear and easy to understand, the patent will be further elaborated below in conjunction with the accompanying drawings and specific implementation methods. The listed embodiments are only to illustrate the present invention and not limit the present invention .

[0021] The trace element mixed solution used in this embodiment contains: 3.0gMgSO 4 ·7H 2 0, 1.0gNaCl, 0.1gCaCl 2 2H 2 O, 0.1g FeSO 4 ·7H 2 O, 0.1 g CoSO 4 , 0.01gAlK(S04) 2 12H 2 O, 0.1gZnSO 4 ·7H 2 O, 0.01g CuSO 4 ·5H 2 O, 0.01gH 3 BO 3 and 0.01gNa 2 MoO 4 2H 2 O.

[0022] (1) Prepare culture medium

[0023] Add 10 grams of glucose, 1.0 grams of Tween 80, 2.56 grams of potassium dihydrogen phosphate, 0.71 grams of magnesium sulfate, 1.56 grams of calcium chloride, and 0.001 grams of vitamin B into the triangular flask 1 , 1.0 g of sodium chloride, 0.5 g of manganese sulfate, and 2.0 m...

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Abstract

The invention discloses a method for degrading bisphenol A through a white-rot fungus crude enzyme. The white-rot fungi crude enzyme is prepared, a tartaric acid buffer solution, a bisphenol A solution, a hydrogen peroxide solution and veratryl alcohol are added, the pH value of a reaction system is controlled to range from 2.5 to 3, the concentration of the veratryl alcohol is 10 mmol / L, the temperature is kept between 20 DEG C and 50 DEG C, the reaction lasts for 30 minutes to 50 minutes, 1.0 mL-3.0 mL methyl alcohol is added for stopping the reaction, and therefore the bisphenol A is degraded through the white-rot fungi crude enzyme. A bio-enzyme catalytic process is adopted in the method, and the method has the beneficial effects of being high in removing efficiency, convenient, safe, free of pollution and the like; and as amboceptor matter is added, the biocatalysis reaction efficiency can be improved, the reaction time can be shortened, the enzyme using amount is reduced, and the running cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of environmental remediation, and in particular relates to a method for degrading bisphenol A by utilizing crude enzyme liquid of microorganism-white rot fungus. Background technique [0002] Bisphenol A (bisphenolA, BPA), the chemical formula is C 15 h 16 o 2 , one of the persistent organic pollutants, has a "three-to-one" effect. The United Nations Environment Program puts it on the agenda as a third-generation environmental problem that needs to be dealt with urgently after the ozone layer hole and global warming. How to reduce this hazard, and what method to degrade such pollutants, or transform them into other non-polluting, less harmful compounds is one of the scientific problems to be solved urgently. The physical adsorption and chemical degradation methods that have been discovered in recent years have good effects on the removal of BPA in the environment, but their disadvantages are also obvious...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A62D3/02C02F3/34
Inventor 刘红艳张飞王秀丽马钰李柏林
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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