Cartilage extracellular matrix and silk fibroin composite orientation cartilage support and preparation method thereof

Abstract

The invention discloses a cartilage extracellular matrix and silk fibroin composite orientation cartilage support and a preparation method thereof and belongs to the biological tissue engineering technology. According to the cartilage extracellular matrix and silk fibroin composite orientation cartilage support and the preparation method of the cartilage support, cartilage extracellular matrixes are prepared through articular cartilage of people, pigs, cows or sheep, silk fibroin is prepared through mulberry silk, after the cartilage extracellular matrixes and the silk fibroin are evenly mixed according to a specific proportion, directed crystallization is performed under a certain temperature, ultraviolet cross linking is firstly performed after freezing and drying, and then cross linking of carbodiimide and N-hydroxysuccinimide or glutaraldehyde and genipin is performed so that the cartilage extracellular matrix and silk fibroin composite orientation cartilage support can be prepared. Due to the design, biocompatibility is good, and immunological rejective reaction is avoided; a composition structure and mechanical properties are similar to those of cartilage extracellular matrixes of people; material sources are wide, cost is low, the preparation technology is simple, the cartilage extracellular matrix and silk fibroin composite orientation cartilage support can be used for constructing tissue engineering cartilage and repairing cartilage degradation, and clinical application prospects are good.

Description

technical field [0001] The invention belongs to biological tissue engineering technology, in particular to a cartilage extracellular matrix and silk fibroin composite orientation cartilage scaffold and a preparation method thereof. Background technique [0002] Biological tissue engineering methods mainly include three elements: scaffold materials, seed cells, and signaling factors. Among them, scaffold materials are the key link in the construction of tissue engineering. An ideal tissue engineering cartilage scaffold material should have the following characteristics: good biocompatibility; appropriate degradability; sufficient pore structure; promote cell adhesion and proliferation; the volume of the scaffold should be able to remain unchanged; Shedding; has a certain degree of elasticity; has the orientation structure of articular cartilage; its composition, shape, and mechanical properties are similar to human cartilage extracellular matrix, etc. As the carrier of seed ...

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of the performance of the existing cartilage tissue engineering scaffold materials and the insufficient pore structure of

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