Method for achieving aggregation of waste incineration fly ash through microorganism mineralization
A waste incineration fly ash and mineralization technology, which is applied in the field of fly ash agglomeration, can solve the problems of difficulty in finding a universal chelating agent, high cost, and large capacity expansion ratio, so as to facilitate the formation, crystallization, and leaching Effect of reducing toxicity and reducing leaching toxicity
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Embodiment 1
[0036] Step 1) Prepare culture medium
[0037] Prepare the culture medium (the culture medium components are shown in Table 1), sterilize at 121°C high temperature, 103kPa high pressure, 20min, let it stand to room temperature, and put Sporosarcinapasteurii (purchased from China General Microorganism Culture Collection Center, No. cgmcc1.3687) was inoculated into a Erlenmeyer flask containing a culture medium at a 1% (v / v) inoculation amount, and cultured on a shaker at 30°C and 220r / min for 46h.
[0038] Table 1 Medium composition
[0039]
[0040] Bacteria solution OD 600 measurement of value
[0041] Bacteria concentration in OD 600The value is expressed, and its value is measured by a UV spectrophotometer. Take 1mL of the bacterial solution cultured on the shaking table (ensure that the operation of the bacterial solution is completed in a sterile environment to avoid contamination of the bacterial solution), and inject it into the culture tube immediately. Take 2 ...
Embodiment 2
[0047] Step 1) Prepare culture medium
[0048] Prepare the culture medium (the composition of the culture medium is shown in Table 1), sterilize at 121°C high temperature, 103kPa high pressure, 15min, and let it stand to room temperature. Culture Collection Center, No. cgmcc1.6503) was inoculated into a Erlenmeyer flask containing medium at a 1% (v / v) inoculum amount, and cultured on a shaker at 20°C and 240r / min for 24h.
[0049] Table 1 Medium composition
[0050]
[0051] Bacteria solution OD 600 measurement of value
[0052] Bacteria concentration in OD 600 The value is expressed, and its value is measured by a UV spectrophotometer. Take 1mL of the bacterial solution cultured on the shaking table (ensure that the operation of the bacterial solution is completed in a sterile environment to avoid contamination of the bacterial solution), and inject it into the culture tube immediately. Take 2 μL of each sample and measure its OD with a UV spectrophotometer 600 value...
Embodiment 3
[0057] Step 1) Prepare culture medium
[0058] Prepare the culture medium (the composition of the culture medium is shown in Table 1), sterilize at 121°C high temperature, 103kPa high pressure, 20min, let it stand to room temperature, and put Bacillus lentus (purchased from China General Microorganism Culture Collection) on the ultra-clean bench. Center, No. cgmcc1.4082) was inoculated into a Erlenmeyer flask containing medium at a 1% (v / v) inoculation amount, and cultured on a shaker at 35° C. and 180 r / min for 48 hours.
[0059] Table 1 Medium composition
[0060]
[0061] Bacteria solution OD 600 measurement of value
[0062] Bacteria concentration in OD 600 The value is expressed, and its value is measured by a UV spectrophotometer. Take 1mL of the bacterial solution cultured on the shaking table (ensure that the operation of the bacterial solution is completed in a sterile environment to avoid contamination of the bacterial solution), and inject it into the culture...
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