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CYP3A5*3 detection kit

A detection kit and the technology of the kit, which are applied in the fields of molecular biology and nucleic acid detection, can solve the problems of high cost, long test time and complicated operation, and achieve the effects of simple operation, short test time and low detection cost.

Inactive Publication Date: 2016-01-27
BEIJING JINQI BIOLOGICAL TECH CO LTD
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Problems solved by technology

[0005] The problem to be solved by the present invention is that the existing method for detecting CYP3A5 genotype has high cost, complicated operation and long testing time

Method used

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Embodiment Construction

[0033] In order to better understand the present invention, the present invention will be further described below in conjunction with specific embodiments and accompanying drawings.

[0034] A detection primer for CYP3A5*3, which includes the following primers:

[0035] 5'-CAACTGCCCTTGCAGCATTT-3' (SEQ ID No.1, hereinafter referred to as P)

[0036] 5'-ACTGTTCTGATCACGTCGGG-3' (SEQ ID No.2, hereinafter referred to as Q)

[0037] 5'-AAAGGCTTTTGTCTTTAAG-3' (SEQ ID No.3, hereinafter referred to as F)

[0038] 5'-GGTCCAAACAGGGAAGAGAGAT-3' (SEQ ID No. 4, hereinafter referred to as R).

[0039] A detection kit for CYP3A5*3, comprising the above-mentioned primers, buffer, Taq enzyme and dNTP. The concentration of each component in the kit can be carried out according to the existing concentration required for PCR. As a preferred solution, in this embodiment, the concentration of each primer is 200 μM, the concentration of dNTP is 100 μM, and the concentration of Taq enzyme is 50 U / ...

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Abstract

The invention provides a CYP3A5*3 detection kit, and belongs to the technical field of molecular biology and nucleic acid detection. The CYP3A5*3 detection kit is designed by employing a bidirectional amplification refractory mutation system PCR (Bi-Amplification Refractory Mutation System PCR, BI-ARMS-PCR) technology on the basis of ARMS-PCR. A specific primer is designed aiming at two alleles of rs776746, and the rs776746 site base in a to-be-detected sample is determined by reading the electrophoresis band quantity and size, and thus whether a tested person carries CYP3A5*3 is determined, and the tested person is guided for medicine usage.

Description

technical field [0001] The invention relates to the technical fields of molecular biology and nucleic acid detection, in particular to a CYP3A5*3 detection kit. Background technique [0002] Cytochrome P450 (cytochrome P450 or CYP450), referred to as CYP450, is a monooxygenase mainly present in the liver and intestinal tract. CYP450 is a group of superfamily enzymes involved in the metabolism of endogenous substances and exogenous compounds in the human body, especially playing an important role in the metabolism of drugs in vivo. Among them, CYP3A5 is involved in the metabolism of various drugs such as tacrolimus, midazolam, dapsone, cortisone, and nifedipine. [0003] A SNP of CYP3A5, rs776746 (CYP3A5*3, 6986A>G), can cause the mRNA of CYP3A5 gene to be cut too short to form an "incomplete" CYP3A5 protein, resulting in the reduction or even disappearance of CYP3A5 enzyme protein activity. The performance of patients carrying CYP3A5 mutant alleles in the course of clin...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6858C12Q2525/185C12Q2531/113C12Q2535/137C12Q2565/125
Inventor 汪大为
Owner BEIJING JINQI BIOLOGICAL TECH CO LTD
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