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Acellular polysaccharide dermal matrix materials and production method and application thereof

A dermal matrix and cell-free technology, applied in the field of bioengineering, can solve the problems of insufficient antibacterial performance and tensile strength to be improved, and achieve the effect of good water vapor permeability, excellent tensile strength and enhanced porosity

Active Publication Date: 2016-02-03
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The acellular dermal matrix is ​​a product obtained by sequentially treating mammalian skin with sodium hydroxide solution and detergent. The acellular dermal matrix treated by this method has the advantages of good histocompatibility and good degradability, but Its tensile strength needs to be improved, and its antibacterial performance is insufficient

Method used

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  • Acellular polysaccharide dermal matrix materials and production method and application thereof
  • Acellular polysaccharide dermal matrix materials and production method and application thereof
  • Acellular polysaccharide dermal matrix materials and production method and application thereof

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Embodiment 1

[0031] This embodiment provides a method for preparing a cell-free polysaccharide dermal matrix dressing, which is prepared by the following steps:

[0032] (1) select porcine acellular dermal matrix (PADM) through sterilized packaging, and accurately weigh as the basis for counting the following procedures;

[0033] (2) Composite carboxymethyl chitosan: Put the prepared acellular dermal matrix into the drum, and add PADM material with a mass ratio of 2.5:1 at a temperature of 40°C and a pH value of 6.0. The mass concentration is 1.00% carboxymethyl chitosan solution, the pH value is adjusted to 5.0 after 30 minutes of reaction, and the pH value is adjusted to 7.0 after another 60 minutes of reaction. Wash the treated material twice with distilled water, 15 minutes each time, and drain the water after washing;

[0034] (3) Glutaraldehyde cross-linking: Add 0.20% glutaraldehyde with a mass ratio of 2.0 to the PADM material under the condition of a temperature of 25°C and a pH va...

Embodiment 2

[0073] This embodiment provides a method for preparing an acellular polysaccharide dermal matrix-epidermal scaffold, which takes the following steps:

[0074] (1) Prepare the acellular polysaccharide dermal matrix material according to the operation of Example 1;

[0075] (2) Composite 5μg / m on the surface of the material 2 growth factor, 10μg / m 2 skin basement membrane formation accelerator, 2.0% glycerin.

[0076] The preparation of the epidermal scaffold by the method can protect the wound, promote the growth of the skin, and improve the healing and regeneration ability of the wound.

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Abstract

The invention relates to acellular polysaccharide dermal matrix materials and a production method and application thereof. The acellular polysaccharide dermal matrix materials comprise acellular dermal matrix materials and polysaccharide, wherein the polysaccharide solution is composite solution of carboxymethyl chitosan, chondroitin sulfate and hyaluronic acid. The production method includes: selecting encapsulated and sterilized PADM, weighing, compositing carboxymethyl chitosan, compositing chondroitin sulfate, crosslinking with glutaraldehyde, compositing hyaluronic acid, washing with a PBS buffer solution, encapsulating, sterilizing, and the like. Functional materials can be added into the acellular polysaccharide dermal matrix materials to produce biologic dressings, composite transplant stents and tissue engineering epidermis stents. The acellular polysaccharide dermal matrix materials are good in in-vitro degradability, high in tensile strength, high in water vapor permeability, high in porosity and excellent in antibacterial performance.

Description

technical field [0001] The invention mainly relates to the field of bioengineering, in particular to a cell-free polysaccharide dermis matrix material and its preparation and application. Background technique [0002] Acellular matrix (acellular dermal matrix, ADM) is obtained by using physical, chemical, biological and other methods to completely remove the epidermis and cells containing antigenic components in the skin and only retain the extracellular matrix containing collagen in the dermis. Originality, good biological properties, and good mechanical properties are ideal biomaterials. Compared with allogeneic acellular dermal matrix, xenogeneic (pig) acellular dermal matrix has the advantages of wide source, low price and high quality, and has the advantages of collagen content, The weaving structure has a high tissue homology and is highly similar to human skin. After modification and cross-linking, its antigenicity can be further reduced, mechanical properties can be ...

Claims

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Application Information

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IPC IPC(8): A61L15/40A61L15/44A61L27/36A61L27/48A61L27/54A61L27/60A61L27/58
Inventor 曹成波王小皎巩柯郭泾
Owner SHANDONG UNIV
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