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Method for extracting high-purity steviol glycosides from stevia rebaudiana

A technology of steviol glycosides and stevia, applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve the problems of unsuitability for industrial production, low product recovery rate, single final product, etc., to facilitate subsequent purification , strong operability, and the effect of increasing the amount of leaching

Active Publication Date: 2016-02-03
HUNAN HUACHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the highest purity of the obtained product can only reach 96%, and the final product is single, the product recovery rate is low, and it is not suitable for industrialized production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Enzyme soaking: crush 50g of stevia dry leaves (containing 4.27% stevioside, 5.52% rebaudioside A, 0.58% rebaudioside C, and 10.37% total sweet glycosides) to 10-30 After that, soak in 510mL of water, add 1g of cellulase preparation for enzymolysis, and obtain stevia rebaudiana enzymatic solution;

[0039] (2) Heat leaching: extract the enzymatic stevia rebaudiana solution obtained in step (1) at 45°C and pH 5.5 for 60 minutes, cool to room temperature, perform three-legged sedimentation centrifugation at a rate of 1200r / min, and filter. Obtain 350mL stevia rebaudiana enzyme extract;

[0040] (3) Flocculation and impurity removal: Put 350mL of stevia plus enzyme extract obtained in step (2) into a 70°C water bath and heat, add 1.05g of inorganic salt flocculant (of which, 0.70g of ferric chloride and 0.35g of calcium oxide) , stirred, and at a pH value of 8.5, after 40 minutes of flocculation treatment, the flocculation solution was first subjected to decanter cent...

Embodiment 2

[0045] (1) Enzyme soaking: crush 50g of stevia dry leaves (containing 4.27% stevioside, 5.52% rebaudioside A, 0.58% rebaudioside C, and 10.37% total sweet glycosides) to 10-30 For this purpose, soak in 500mL water, add 1g cellulase preparation for enzymolysis, and obtain stevia rebaudiana enzymatic solution;

[0046] (2) Heat leaching: extract the enzymatic stevia rebaudiana solution obtained in step (1) at 43°C and pH 5.0 for 65 minutes, cool to room temperature, perform three-legged sedimentation centrifugation at a rate of 1000r / min, and filter. Obtain 351mL stevia rebaudiana enzyme extract;

[0047] (3) Flocculation and impurity removal: Heat 351mL of stevia-enzyme extract obtained in step (2) in a water bath at 68°C, and add 1.2285g of inorganic salt flocculant (including 0.6825g of ferric chloride and 0.546g of calcium oxide) , stirred, and at a pH value of 8.0, after 30 minutes of flocculation treatment, the flocculation solution was first subjected to decanter centrif...

Embodiment 3

[0052] (1) Enzyme soaking: crush 100g of stevia dry leaves (containing 4.27% stevioside, 5.52% rebaudioside A, 0.58% rebaudioside C, and 10.37% total sweet glycosides) to 10-30 For the purpose, soak in 1050mL water, add 2g of cellulase preparation for enzymolysis, and obtain stevia rebaudiana enzymatic solution;

[0053] (2) Heat leaching: extract the stevia rebaudiana hydrolyzate obtained in step (1) at 46°C and pH 6.0 for 70 minutes, cool to room temperature, perform three-legged sedimentation centrifugation at 1500r / min, filter, Obtain 746mL stevia rebaudiana enzyme extract;

[0054] (3) Flocculation and impurity removal: Put 746mL of stevia-enzyme extract obtained in step (2) into a water bath at 72°C to heat, and add 2.984g of inorganic salt flocculant (including 1.492g of ferric chloride and 1.492g of calcium oxide) , stirred, and at a pH value of 9.0, after 35 minutes of flocculation treatment, the flocculation solution was first subjected to decanter centrifugation at...

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Abstract

The invention relates to a method for extracting high-purity steviol glycosides from stevia rebaudiana. The method comprises the following steps: (1) soaking a ground stevia rebaudiana raw material into water, and adding a cellulase preparation for enzymolysis; (2) carrying out heating extraction and centrifugation, and filtering, so as to obtain stevia rebaudiana enzyme extraction liquid; (3) carrying out water-bath heating on the stevia rebaudiana enzyme extraction liquid, adding an inorganic salt flocculating agent, stirring, flocculating, carrying out centrifugation, and filtering, so as to obtain stevia rebaudiana edulcorated liquid; (4) carrying out decolorization adsorption on the stevia rebaudiana edulcorated liquid by virtue of a macroporous adsorption resin column, carrying out elution through eluent, carrying out nanofiltration, and concentrating, so as to obtain a stevia rebaudiana concentrated liquid; and (5) carrying out silica-gel column chromatography and elution on the stevia rebaudiana concentrated liquid, collecting each fraction, concentrating, and drying, so as to obtain multiple high-purity steviol glycosides products. The purity of the products obtained by virtue of the method can reach up to 98.4%, and the final yield is more than 90%; the residual amounts of ethanol and ethyl ester are low, so that the method is environmentally friendly and safe; the method is simple in process, high in operability, low in energy consumption and cost and applicable to industrial production.

Description

technical field [0001] The invention relates to a method for extracting high-purity steviol glycosides, in particular to a method for extracting high-purity steviol glycosides from stevia which is suitable for industrial production. Background technique [0002] Stevia is native to the Amambay and Mbaxacayu mountains in Paraguay. It belongs to the sugar family. The temperature during the entire growth period is above 20°C, and it is thermophilic. In 1970, Japan introduced stevia from Brazil, began domestication, cultivation, and glycoside production, and at the same time conducted toxicology, food inspection and other tests, and first developed and utilized stevia products - steviol glycosides. Our country introduced from Japan in 1976, tried planting, and achieved success, and promoted planting to all parts of the country in the early 1980s. [0003] Stevioside is a class of tetracyclic diterpenoid derivatives mainly found in Stevia rebaudiana leaves, mainly including Stev...

Claims

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Application Information

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IPC IPC(8): C07H15/256C07H1/08
Inventor 龙伟岸黄华学
Owner HUNAN HUACHENG BIOTECH
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