Method for extracting high-purity steviol glycosides from stevia rebaudiana

A technology of steviol glycosides and stevia, applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve the problems of unsuitability for industrial production, low product recovery rate, single final product, etc., to facilitate subsequent purification , strong operability, and the effect of increasing the amount of leaching

Active Publication Date: 2016-02-03
HUNAN HUACHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the highest purity of the obtained product can only reach 96%, and the final product i

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0037] Example 1

[0038] (1) Enzyme soaking: 50g dry leaves of stevia (containing 4.27% of stevioside, 5.52% of rebaudioside A, 0.58% of rebaudioside C, and 10.37% of total glycosides) are crushed to 10~30 After finishing, soak in 510mL water, add 1g cellulase preparation for enzymatic hydrolysis to obtain stevia enzymatic hydrolysate;

[0039] (2) Heating extraction: The stevia enzymatic hydrolysate obtained in step (1) was extracted at 45°C and pH 5.5 for 60 minutes, cooled to room temperature, and subjected to three-leg centrifugation and filtration at a rate of 1200r / min. Obtain 350mL stevia with enzyme extract;

[0040] (3) Flocculation and impurity removal: Put 350mL of the stevia enzyme extract obtained in step (2) into a 70℃ water bath and heat it, add 1.05g of inorganic salt flocculant (of which, iron chloride 0.70g, calcium oxide 0.35g) , Stir, at pH 8.5, after flocculation treatment for 40 minutes, first decanter the flocculation solution at a rate of 3000r / min, and the...

Example Embodiment

[0044] Example 2

[0045] (1) Enzyme soaking: 50g dry leaves of stevia (containing 4.27% of stevioside, 5.52% of rebaudioside A, 0.58% of rebaudioside C, and 10.37% of total glycosides) are crushed to 10~30 Soak in 500mL water, add 1g cellulase preparation for enzymolysis to obtain stevia enzymatic hydrolysate;

[0046] (2) Heating extraction: The stevia enzymatic hydrolysate obtained in step (1) was extracted at 43°C and pH 5.0 for 65 minutes, cooled to room temperature, and subjected to three-leg centrifugation and filtration at a rate of 1000r / min. Obtain 351mL stevia with enzyme extract;

[0047] (3) Flocculation and impurity removal: Put 351 mL of the stevia extract obtained in step (2) into a 68°C water bath for heating, and add 1.2285 g of inorganic salt flocculant (of which, ferric chloride 0.6825 g, calcium oxide 0.546 g) , Stirring, at pH 8.0, after 30min of flocculation treatment, the flocculation solution is first centrifuged at a rate of 2500r / min, and then centrifuge...

Example Embodiment

[0051] Example 3

[0052] (1) Enzyme soaking: 100g dry leaves of stevia (containing 4.27% of stevioside, 5.52% of rebaudioside A, 0.58% of rebaudioside C, 0.58% of rebaudioside, 10.37% of total glycosides) are crushed to 10~30 Soak in 1050mL water, add 2g cellulase preparation for enzymatic hydrolysis to obtain stevia enzymatic hydrolysate;

[0053] (2) Heating extraction: The stevia enzymatic hydrolysate obtained in step (1) is extracted at 46°C and pH 6.0 for 70 minutes, cooled to room temperature, and subjected to three-legged centrifugation and filtration at a rate of 1500r / min. Obtain 746mL stevia with enzyme extract;

[0054] (3) Flocculation and impurity removal: Put 746 mL of the stevia extract obtained in step (2) into a 72°C water bath for heating, and add 2.984 g of inorganic salt flocculant (including iron chloride 1.492 g and calcium oxide 1.492 g) , Stir, at pH 9.0, after 35min of flocculation treatment, the flocculation solution is first subjected to decanter centri...

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Abstract

The invention relates to a method for extracting high-purity steviol glycosides from stevia rebaudiana. The method comprises the following steps: (1) soaking a ground stevia rebaudiana raw material into water, and adding a cellulase preparation for enzymolysis; (2) carrying out heating extraction and centrifugation, and filtering, so as to obtain stevia rebaudiana enzyme extraction liquid; (3) carrying out water-bath heating on the stevia rebaudiana enzyme extraction liquid, adding an inorganic salt flocculating agent, stirring, flocculating, carrying out centrifugation, and filtering, so as to obtain stevia rebaudiana edulcorated liquid; (4) carrying out decolorization adsorption on the stevia rebaudiana edulcorated liquid by virtue of a macroporous adsorption resin column, carrying out elution through eluent, carrying out nanofiltration, and concentrating, so as to obtain a stevia rebaudiana concentrated liquid; and (5) carrying out silica-gel column chromatography and elution on the stevia rebaudiana concentrated liquid, collecting each fraction, concentrating, and drying, so as to obtain multiple high-purity steviol glycosides products. The purity of the products obtained by virtue of the method can reach up to 98.4%, and the final yield is more than 90%; the residual amounts of ethanol and ethyl ester are low, so that the method is environmentally friendly and safe; the method is simple in process, high in operability, low in energy consumption and cost and applicable to industrial production.

Description

technical field [0001] The invention relates to a method for extracting high-purity steviol glycosides, in particular to a method for extracting high-purity steviol glycosides from stevia which is suitable for industrial production. Background technique [0002] Stevia is native to the Amambay and Mbaxacayu mountains in Paraguay. It belongs to the sugar family. The temperature during the entire growth period is above 20°C, and it is thermophilic. In 1970, Japan introduced stevia from Brazil, began domestication, cultivation, and glycoside production, and at the same time conducted toxicology, food inspection and other tests, and first developed and utilized stevia products - steviol glycosides. Our country introduced from Japan in 1976, tried planting, and achieved success, and promoted planting to all parts of the country in the early 1980s. [0003] Stevioside is a class of tetracyclic diterpenoid derivatives mainly found in Stevia rebaudiana leaves, mainly including Stev...

Claims

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Application Information

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IPC IPC(8): C07H15/256C07H1/08
Inventor 龙伟岸黄华学
Owner HUNAN HUACHENG BIOTECH
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